Search results for "Reservation"
showing 10 items of 517 documents
Sapropel as a Binder: Properties and Application Possibilities for Composite Materials
2015
Recent development trends largely look for possibilities of a wider use of natural materials and local resources. In this perspective, the use of organic rich lake sediment - sapropel - as a binding material in line with other environmentally friendly filling materials can be considered as a challenge. Sapropel itself is a valuable resource with multiple areas of application, for example, medicine, veterinary, agriculture, livestock farming, balneology, cosmetic applications, construction, and its application options have been widely studied in the 20th century in the Baltic countries, Ukraine and Russia. Birch wood fibre and sanding dust, hemp shives, 'Aerosil' are used as a filler and thr…
Reventilation Episodes During the Sapropel S1 Deposition in the Eastern Mediterranean Based on Holococcolith Preservation
2019
Organic-rich layers (sapropels), preserved in eastern Mediterranean marine sediment records, represent pronounced perturbations to thermohaline circulation and environmental conditions in the basin, in response to enhanced African monsoon activity and subsequent massive freshwater discharge. During the most recent event, Sapropel S1 formed between 10.8 and 6.1 ka, when freshwater-driven stratification caused seafloor anoxia below ~1,800-m depth, as a result of both failure of deep water formation and enhanced productivity. Here we analyze coccolith assemblages from the open eastern Mediterranean that form a west-east transect across the basin and provide insights on past environmental chang…
DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicl…
2010
Cryopreservation of follicles for culture and oocyte growth and maturation in vitro provides an option to increase the number of fertilizable oocytes and restore fertility in cases where transplantation of ovarian tissue poses a risk for malignant cell contamination. Vitrification for cryopreservation is fast and avoids ice crystal formation. However, the influences of exposure to high concentrations of cryoprotectants on follicle development, oocyte growth and maturation, and particularly, on the DNA integrity and methylation imprinting has not been studied systematically. Follicle survival and development, DNA damage, oocyte growth patterns, maturation, spindle formation and chromosomal c…
Investigating a simplified method for noninvasive genetic sampling in East African mammals using silica dried scat swabs
2020
Abstract Swabbing scat has proved to be an effective noninvasive method to collect DNA from mammals in the field. Previously, this method has relied on preservative liquids or freezing to preserve the DNA collected on swabs. In this study, we determine the effectiveness of using silica to simply dry the swab in field as an alternative way to prevent DNA degredation. Four species were included in the study; reticulated giraffe, impala, fringe‐eared oryx, and lion. Swabs were taken at multiple time points for giraffe and impala scat samples, with the lion and oryx sampled opportunistically. Mitochondrial DNA was successfully amplified and sequenced from scat swabs from all species; however, e…
Lactic acid bacteria associated with vacuum-packed cooked meat product spoilage: population analysis by rDNA-based methods.
2007
Aim: To determine the lactic acid bacteria (LAB) implicated in bloating spoilage of vacuum-packed and refrigerated meat products. Methods and Results: A total of 18 samples corresponding to four types of meat products, with and without spoilage symptoms, were studied. In all, 387 colonies growing on de Man, Rogosa and Sharpe, yeast glucose lactose peptone and trypticase soy yeast extract plates were identified by internal spacer region (ISR), ISR-restriction fragment length polymorphism and rapid amplified ribosomal DNA restriction analysis profiles as Lactobacillus (37%), Leuconostoc (43%), Carnobacterium (11%), Enterococcus (4%) and Lactococcus (2%). Leuconostoc mesenteroides dominated …
Salt drying: a low-cost, simple and efficient method for storing plants in the field and preserving biological repositories for DNA diversity researc…
2013
Although a variety of methods have been optimized for the collection and storage of plant specimens, most of these are not suited for field expeditions for a variety of logistic reasons. Drying specimens with silica gel in polyethylene bags is currently the standard for field-sampling methods that are suitable for subsequent DNA extraction. However, silica-gel repositories are not readily available in remote areas, and its use is not very cost-effective for the long-term storage of collections or in developing countries with limited research budgets. Salting is an ancient and traditional drying process that preserves food samples by dehydrating tissues and inhibiting water-dependent cellula…
The influence of platform switching in dental implants. A literature review.
2010
Introduction: The platform switching concept involves the reduction of the restoration abutment diameter with respect to the diameter of the dental implant. In 1991, dental implants of 5 and 6 mm diameter platforms were introduced. However, matching-diameter prosthetic abutments were not available. These implants were restored with “standard”-diameter prosthetic components (4,1 mm). Long-term follow up around these wide-platforms showed higher levels of bone preservation. In time, it has been called platform switching. The first case report applying this concept was indexed in MedLine in 2005. Materials and Methods: The aim of this article is to carry out a literature review of articles whi…
La teoria della subalternità e il caso dei dalit in India
2008
Il subalterno è lo schiavo soddisfatto, il servo che idolatra il padrone, la donna che perpetua il modello del patriarca, il marginale che finisce per odiarsi: colui che ha interiorizzato la visione del mondo del dominante e crede legittimo il suo stato di minorità. Egli, subendo la cosiddetta violenza epistemica, si fa complice di chi rende angusta la sua esistenza: le fonti del suo istinto sono inquinate, alterate le ragioni dei suoi desideri ed egli vuole contro il proprio interesse. I subalterni sono il neo delle rivoluzioni e la falla di ogni progresso: quella porzione di popolazione che ha partecipato ma non ha compreso, le cui condizioni di vita non sono cambiate, subordinati prima a…
First trimester biochemical screening for Down's syndrome in singleton pregnancies conceived by assisted reproduction
2005
BACKGROUND: Serum biochemical markers [free betahCG (fbetahCG); pregnancy-associated plasma protein-A (PAPP-A)] used in first trimester Down's syndrome screening have not been fully investigated in pregnancies achieved by assisted reproduction techniques. We present data on pregnancies conceived by all types of assisted reproduction techniques, including pregnancies following ovum donation (OD) and a large sample by ICSI. METHODS: First trimester Down's syndrome screening was performed in 1054 normal singleton pregnancies: natural conception (n = 498), ovulation induction (OS, n = 97), IVF (n = 47), ICSI (n = 222) and OD (n = 190). RESULTS: No differences in maternal levels of fbetahCG and …
Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature.
1999
Abstract Embedding biostructures in saccharide glasses protects them against extreme dehydration and/or exposure to very high temperature. Among the saccharides, trehalose appears to be the most effective bioprotectant. In this paper we report on the low-frequency dynamics of carbon monoxy myoglobin in an extremely dry trehalose glass measured by neutron spectroscopy. Under these conditions, the mean square displacements and the density of state function are those of a harmonic solid, up to room temperature, in contrast to D 2 O-hydrated myoglobin, in which a dynamical transition to a nonharmonic regime has been observed at ∼180K (Doster et al., 1989. Nature. 337:754–756). The protective ef…