Search results for "Respirovirus"

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Evaluation of a multiplex reverse transcriptase PCR ELISA for the detection of nine respiratory tract pathogens.

2003

A multiplex reverse transcription (RT) polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) was previously developed to detect nine different microorganisms: enterovirus (EV), influenza virus type A (IVA) and type B (IVB), respiratory syncytial virus (RSV), parainfluenzavirus type 1 (PIV1) and type 3 (PIV3), adenovirus (AV), Mycoplasma pneumoniae (Mpn), Chlamydia pneumoniae (Cpn) in a single test. These organisms do not usually colonize the respiratory tract of humans, but, if present, it may be assumed they are involved in respiratory disease.The m-RT-PCR-ELISA was tested on (i) culture supernatants of unknown contents, (ii) by determining the analytical…

Enzyme-Linked Immunosorbent AssayBiologymedicine.disease_causeSensitivity and SpecificityRespirovirusMicrobiologyAdenoviridaeVirologyMultiplex polymerase chain reactionmedicineHumansMultiplexRespiratory Tract InfectionsEnterovirusObserver VariationRespiratory tract infectionsReverse Transcriptase Polymerase Chain ReactionReproducibility of ResultsVirologyReverse transcriptaseRespiratory Syncytial VirusesReverse transcription polymerase chain reactionInfluenza B virusInfectious Diseasesmedicine.anatomical_structureRespiratory syncytial virus (RSV)Influenza A virusDNA ViralEnterovirusRespiratory tractJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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Homologous recombination as a mechanism of genetic changes in bovine parainfluenza-3 virus

2021

Bovine parainfluenza-3 virus (BPIV-3) is one of the main viruses associated with bovine respiratory disease complex (BRDC) worldwide. BPIV-3 infect the bovine respiratory tract causing from subclinical infections to severe pneumonia with significant economic losses in the cattle industry. BPIV-3 is a RNA virus with high genetic variability, nevertheless, the contribution of recombination events to its variability has not been assessed so far. In this study the 25 complete genome sequences (CGS) reported so far and 215 partial sequences of different viral genes of BPIV-3 were analyzed to determine their genotypes and subgenotypes, distribution, and the existence of potential recombination ev…

GenotypeCattle DiseasesSheep DiseasesBiologyRespirovirus InfectionsMicrobiologyGenomeVirusViral ProteinsAnimalsGenetic variabilityHomologous RecombinationGeneParainfluenza Virus 3 BovinePhylogenyGeneticsSheepGeneral VeterinaryPhylogenetic treeGenetic VariationRNA virusGeneral Medicinebiology.organism_classificationBovine Respiratory Disease ComplexCattleHomologous recombinationVeterinary Microbiology
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