Search results for "Response"

showing 10 items of 4136 documents

Wee1 inhibition potentiates Wip1-dependent p53-negative tumor cell death during chemotherapy

2016

AbstractInactivation of p53 found in more than half of human cancers is often associated with increased tumor resistance to anti-cancer therapy. We have previously shown that overexpression of the phosphatase Wip1 in p53-negative tumors sensitizes them to chemotherapeutic agents, while protecting normal tissues from the side effects of anti-cancer treatment. In this study, we decided to search for kinases that prevent Wip1-mediated sensitization of cancer cells, thereby interfering with efficacy of genotoxic anti-cancer drugs. To this end, we performed a flow cytometry-based screening in order to identify kinases that regulated the levels of γH2AX, which were used as readout. Another criter…

Wip1ApoptosisCell Cycle ProteinsPharmacologyMESH: G2 Phase Cell Cycle CheckpointsHistonesMESH : PhosphorylationMiceMESH : Cell Cycle ProteinsMESH: AnimalsMESH: Tumor Suppressor Protein p53MESH: HistonesKinaseTp53 mutationsMESH : Mice Transgenic3. Good healthProtein Phosphatase 2CSurvival RateMESH : Antineoplastic AgentsH2ax phosphorylationP53 activationMESH: Protein Phosphatase 2CRNA InterferenceMESH : Colorectal NeoplasmsMESH : Carrier ProteinsHistone H2axMESH: MitochondriaImmunologyHuman fibroblastsMESH: Carrier ProteinsAntineoplastic AgentsMESH: Protein-Tyrosine KinasesMESH: Protein-Serine-Threonine KinasesMESH : Cisplatin03 medical and health sciencesMESH: Cell Cycle ProteinsGenotoxic stressMESH : Protein-Tyrosine KinasesHumansMESH : HistonesAnticancer TherapyMESH: DNA DamageCisplatinMESH: HumansMESH: Phosphorylation[ SDV.BC ] Life Sciences [q-bio]/Cellular BiologyMESH : HumansMESH : Nuclear Proteins030104 developmental biologyCancer cellMESH: Antineoplastic AgentsCisplatinCarrier ProteinsMESH: Nuclear ProteinsMESH : ApoptosisDna-damage response0301 basic medicineCancer ResearchMESH: Caspase 3MESH : Caspase 3PhosphorylationCytotoxicityMESH : DNA DamageSensitizationmedicine.diagnostic_testCaspase 3Nuclear ProteinsProtein-Tyrosine KinasesMESH : Survival RateMitochondriaG2 Phase Cell Cycle CheckpointsWee1medicine.anatomical_structureMESH : Protein Phosphatase 2COriginal ArticleMESH : MitochondriaColorectal Neoplasmsmedicine.drugMESH : Protein-Serine-Threonine KinasesMESH: Cell Line TumorMESH: Survival RateMESH: Mice TransgenicMESH: RNA InterferencePhosphataseMice Transgenic[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyProtein Serine-Threonine KinasesFlow cytometryCellular and Molecular NeuroscienceCell Line TumorMESH : MicemedicineAnimalsMESH: MiceMESH : Cell Line TumorMESH: ApoptosisCell BiologyMESH : Tumor Suppressor Protein p53MESH: CisplatinCancer researchbiology.proteinMESH : AnimalsMESH : G2 Phase Cell Cycle CheckpointsMESH : RNA InterferenceTumor Suppressor Protein p53MESH: Colorectal NeoplasmsDNA DamageCell Death & Disease
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Respuestas psicobiológicas en profesores al inicio y al final de un curso académico

2013

La evidencia científica indica que el estrés laboral incrementa el riesgo de enfermedad cardiovascular. Las respuestas emocionales, cardiovasculares y endocrinas se ven afectadas por la experiencia diaria. La percepción de estrés, el estado de ánimo negativo, la frecuencia cardíaca (FC) y la presión arterial (PA) incrementan en los periodos laborales. La respuesta del cortisol (C) no es tan clara, existiendo resultados contradictorios. El objetivo de este trabajo es analizar en profesores los marcadores de estrés durante dos jornadas laborales. Además se evaluó el papel del género en la respuesta de estrés en 49 profesores. Para ello, se midió la percepción de estrés, el estado de ánimo, la…

Work stressGynecologymedicine.medical_specialtyteachersmoodcortisolcardiovascular responseDevelopmental psychology159.9 - PsicologíaNegative moodSchool teachersMoodWork stressHeart rateprofesoresmedicineEstado anímicoestado de ánimoEstrés Laboralrespuesta cardiovascularPsychologyGeneral PsychologyAnales de Psicología
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Psychobiological responses at the beginning and the end of an academic year in teachers

2013

Evidence shows that work stress increases risk of cardiovascular disease. Emotional, cardiovascular and endocrine responses are affected by day-to-day experience. Perceived stress, negative mood, heart rate (HR) and blood pressure (BP) increase in working periods. Cortisol (C) response is not so clear, there being contradictory results. The main purpose is to study stress markers in two working days in teachers. Moreover, we evaluate the role of gender in stress markers in 49 school teachers. Perceived stress, mood, BP, HR, and C were measured at the beginning and at the end of an academic year. Results show that psychological and heart rate responses to a working day were different at the …

Work stressteacherslcsh:Psychologymoodlcsh:BF1-990profesoresestado de ánimoEstrés Laboralcortisolcardiovascular responserespuesta cardiovascular
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Burnout syndrome among psychiatric trainees in 22 countries: Risk increased by long working hours, lack of supervision, and psychiatry not being firs…

2016

AbstractBackgroundPostgraduate medical trainees experience high rates of burnout, but evidence regarding psychiatric trainees is missing. We aim to determine burnout rates among psychiatric trainees, and identify individual, educational and work-related factors associated with severe burnout.MethodsIn an online survey psychiatric trainees from 22 countries were asked to complete the Maslach Burnout Inventory (MBI-GS) and provide information on individual, educational and work-related parameters. Linear mixed models were used to predict the MBI-GS scores, and a generalized linear mixed model to predict severe burnout.ResultsThis is the largest study on burnout and training conditions among p…

Working hoursAdultMaleMental Health ServicesComplete datamedicine.medical_specialtyBurnout; Psychiatry; Risk factors; Supervision; Training; Adult; Career Choice; Demography; Education Medical Continuing; Female; Humans; Male; Mental Health Services; Middle Aged; Personality Inventory; Psychiatry; Risk Factors; Socioeconomic Factors; Surveys and Questionnaires; Work Schedule Tolerance; Burnout Professional; Psychiatry and Mental HealthPersonality Inventoryhealth care facilities manpower and servicesBurnout syndromeeducationBurnout03 medical and health sciences0302 clinical medicineRisk FactorsSurveys and QuestionnairesWork Schedule TolerancemedicineHumans030212 general & internal medicinePsychiatryBurnout ProfessionalDemographyHigh rateResponse rate (survey)PsychiatryCareer Choicebusiness.industryMiddle Aged030227 psychiatryPsychiatry and Mental healthSocioeconomic FactorsEducation Medical ContinuingFemalePersonality Assessment Inventorybusinesspsychological phenomena and processesCareer choiceClinical psychologyEuropean psychiatry : the journal of the Association of European Psychiatrists
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Depletion ofL-arginine induces autophagy as a cytoprotective response to endoplasmic reticulum stress in human T lymphocytes

2012

PMCID: PMC3494587

X-Box Binding Protein 1Proteasome Endopeptidase ComplexProgrammed cell deathXBP1CD3 ComplexMAP Kinase Signaling SystemRNA SplicingT-LymphocytesT cellDown-RegulationApoptosisRegulatory Factor X Transcription FactorsUbiquitin-Activating EnzymesProtein Serine-Threonine KinasesBiologyArginineLymphocyte ActivationAutophagy-Related Protein 7Jurkat cellsJurkat CellsEndoribonucleasesAutophagymedicineHumansMolecular BiologyCell ProliferationTOR Serine-Threonine KinasesAutophagyMembrane ProteinsCell BiologyBECN1Endoplasmic Reticulum StressG1 Phase Cell Cycle CheckpointsBasic Research Paper3. Good healthCell biologyDNA-Binding Proteinsmedicine.anatomical_structureCytoprotectionApoptosisUnfolded protein responseBeclin-1MitogensApoptosis Regulatory ProteinsLysosomesProto-Oncogene Proteins c-aktTranscription FactorsAutophagy
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OP0204 Autophagy and Unfolded Protein Response: A Fine Balance that can Influence the Pathogenesis of Ankylosing Spondylitis and Inflammatory Bowel D…

2015

Background We have shown an increase in the unfolded protein response (UPR) with decreased ERAP1 or ERAP2 function in an in vitro system. Similarly UPR has been demonstrated to correlate with onset of disease in the HLA-B27 rat model. UPR has been difficult to demonstrate in the gut of AS patients but autophagy is upregulated. ERAP2 is associated with both AS and inflammatory bowel disease (IBD). Objectives Here we explore the moderating effect of autophagy on UPR. Specifically we study the impact of suppressing autophagy on UPR. Methods Lamina Propria Mononuclear cells (LPMC) were isolated from terminal ileal biopsies of 10 AS patients. Autophagy was suppressed with 2 agents anisomycin and…

XBP1biologymedicine.diagnostic_testImmunologyAutophagyMajor histocompatibility complexdigestive systemGeneral Biochemistry Genetics and Molecular BiologyCell biologyFlow cytometryPathogenesischemistry.chemical_compoundRheumatologychemistryDownregulation and upregulationbiology.proteinUnfolded protein responsemedicineImmunology and AllergyAnisomycinAnnals of the Rheumatic Diseases
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Flavonols and flavan-3-ols as modulators of xanthine oxidase and manganese superoxide dismutase activity.

2014

Experiments were performed to assess the dose-dependent effects of quercetin, kaempferol, (+) catechin, and (-) epicatechin on superoxide radical production through the modulation of manganese superoxide dismutase and xanthine oxidase activities. The experiments were carried out at flavanoid concentrations ranging from 1 µM to 100 µM. This investigation highlighted that flavonols induced opposite effects on superoxide radical production at different doses, i.e. pro-oxidant at the highest concentration (100 µM) and anti-oxidant at the lowest concentration (1 µM). Similar behaviors were observed for xanthine oxidase with flavan-3ols. The diastereoisomer (the catechin) acted as a stronger radi…

Xanthine OxidaseAntioxidantInhibitorFlavonolsmedicine.medical_treatmentPro-oxidantSettore BIO/09 - FisiologiaSuperoxide dismutasechemistry.chemical_compoundFlavonolsFlavanDose responsemedicineXanthine oxidasechemistry.chemical_classificationFlavonoidsbiologyMolecular StructureSuperoxide DismutaseMedicine (all)Functional foodCatechinPro-oxidantStructure-Activity relationshipchemistryBiochemistrybiology.proteinFlavonoidSettore BIO/14 - FarmacologiaAntioxidantKaempferolFlavonolFood ScienceInternational journal of food sciences and nutrition
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Toxicity of fatty acid hydroperoxides towards Yarrowia lipolytica: Implication of their membrane fluidizing action

2007

Abstract Linoleic acid hydroperoxide (HPOD), substrate of hydroperoxide lyase, an enzyme of the lipoxygenase pathway, can be transformed into many aromatic compounds, the so-called “green notes”. The presence of linoleic acid hydroperoxide in the culture medium of Yarrowia lipolytica, the yeast expressing the cloned hydroperoxide lyase of green bell pepper, undoubtedly exerted an inhibition on the growth and a toxic effect with 90% of yeast cells died after 120 min of exposition in 100 mM HPOD solution. The increase in cell membrane fluidity evaluated by measuring fluorescence generalized polarization with the increasing concentration of HPOD in the medium confirmed the fluidizing action of…

Yarrowia lipolyticaLipid PeroxidesBiophysicsYarrowiaBiochemistryCell membraneLipoxygenasechemistry.chemical_compoundMembrane fluiditymedicineMembrane fluidityInfrared spectroscopyPhospholipidsLaurdanbiologyDose-Response Relationship DrugToxicityChemistryCell MembraneSubstrate (chemistry)YarrowiaCell Biologybiology.organism_classificationYeastMembranemedicine.anatomical_structureBiochemistryLinoleic Acidsbiology.proteinLaurdanBiochimica et Biophysica Acta (BBA) - Biomembranes
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Ambiguous Role of Interleukin-12 in Yersinia enterocolitica Infection in Susceptible and Resistant Mouse Strains

1998

ABSTRACT Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-γ) production in NK and CD4 + T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia -susceptible BALB/c mice but exacerbates yersiniosis in resistant C57BL/6 mice. Therefore, we wanted to dissect the different mechanisms exerted by IL-12 during Yersinia infections by using different models of Yersinia -resistant and -susceptible mice, including resistant C57BL/6 mice, susceptible BALB/c mice, intermediate-susceptible wild-type 129/Sv mice, 129/Sv IFN-γ-receptor-deficient (IFN-γR −/− ) mice and C57BL/6 tumor n…

Yersinia Infectionsmedicine.medical_treatmentImmunologyCD8-Positive T-LymphocytesYersiniaMicrobiologyMicrobiologyProinflammatory cytokineInterferon-gammaMiceTransforming Growth Factor betamedicineAnimalsInterferon gammaYersinia enterocoliticaReceptors InterferonYersinia enterocoliticaMice KnockoutHost Response and InflammationMice Inbred BALB CbiologyTumor Necrosis Factor-alphaYersiniosisbiology.organism_classificationmedicine.diseaseInterleukin-12Killer Cells NaturalMice Inbred C57BLInfectious DiseasesCytokineImmunologyInterleukin 12FemaleParasitologyTumor necrosis factor alphamedicine.drugInfection and Immunity
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Mining extraction in the ocean depths: a baseline to understand and reduce acoustic impact on biodiversity.

2020

[ES] A lo largo de la historia, el hombre ha explotado los recursos minerales de la tierra para su supervivencia y desarrollo tecnológico sin un equilibrio con su regeneración. Dado el crecimiento de la población mundial y la reducción de recursos, el hombre comenzó a buscar nuevos depósitos que se encontraron en la década de 1960 en las profundidades de los océanos. Con estos, la humanidad empezò a pensar en extraer los minerales de estos depósitos y esto llevò al nacimiento de Deep Sea Mining (DSM). Las consecuencias de las actividades mineras en las profundidades del mar no se conocen realmente y los efectos pueden ser diferentes: contaminación acústica, contaminación lumínica, contamina…

ZOOLOGIAbiologyHolothuria tubulosanoise pollutionZoologyphysiological responses.Physiological responsesJuvenile fishbiology.organism_classificationChromis chromisDeep Sea Miningbiochemical parameterDeep sea miningBiochemical parametersHabitat destructionNoise pollutionbiology.animalFISICA APLICADAbehavioural responseBehavioural responsesSettore BIO/06 - Anatomia Comparata E CitologiaArbacia lixulaSea urchinInvertebrate
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