Search results for "Restriction Fragment Length"

showing 10 items of 200 documents

Yeast ecology of vineyards within Marsala wine area (western Sicily) in two consecutive vintages and selection of autochthonous Saccharomyces cerevis…

2012

In this work, the yeast ecology associated with the spontaneous fermentation of Grillo cultivar grapes from 10 vineyards was analyzed from grape harvest till complete consumption of must sugars. The microbiological investigation started with the plate count onto two culture media to distinguish total yeasts (TY) and presumptive Saccharomyces (PS). Yeasts were randomly isolated and identified by a combined genotypic approach consisting of restriction fragment length polymorphism (RFLP) of 5.8S rRNA gene and 26S rRNA and sequencing of D1/D2 domain of the 26S rRNA gene, which resulted in the recognition of 14 species belonging to 10 genera. The distribution of the yeasts within the vineyards s…

IdentificationGenotypeSaccharomyces cerevisiaeAcetic Acid; Culture Media; DNA Fungal; Ethanol; Fermentation; Genotype; Hydrogen Sulfide; Microsatellite Repeats; Polymerase Chain Reaction; Polymorphism Restriction Fragment Length; RNA Ribosomal; Saccharomyces cerevisiae; Sicily; Sulfites; Temperature; Vitis; WineBioengineeringWineSaccharomyces cerevisiaeBiologyApplied Microbiology and BiotechnologySaccharomycesPolymerase Chain ReactionEnological aptitudeYeastsGenotypeSulfitesVitisHydrogen SulfidePolymorphismDNA FungalSicilyAcetic AcidRibosomalWineEthanolEcologyIdentification; Enological aptitudes; Saccharomyces cerevisiae; Spontaneous wine fermentation; YeastsTemperatureDNARibosomal RNASpontaneous wine fermentationbiology.organism_classificationYeastCulture MediaFungalRestriction Fragment LengthRNA RibosomalFermentationRNAFermentationRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthBiotechnologySettore AGR/16 - Microbiologia AgrariaMicrosatellite RepeatsJournal of bioscience and bioengineering
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Typing of anastomosis groups of Rhizoctonia solani by restriction analysis of ribosomal DNA.

2003

A method based on restriction analysis of polymerase chain reaction (PCR)-amplified ribosomal DNA was developed for the rapid characterization of large populations of Rhizoctonia solani at the anastomosis group (AG) level. The restriction maps of the internal transcribed spacers (ITS) sequences were compared for 219 isolates of R. solani belonging to AG-1 to AG-12 and AG-BI, representing diverse geographic and host range origins. Four discriminant restriction enzymes (MseI, AvaII, HincII, and MunI) resolved 40 restriction fragment length polymorphism (RFLP) types among the 219 ITS sequences of R. solani. Each RFLP type could be assigned to a single AG except for two RFLP types, which were c…

ImmunologyApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalPolymerase Chain ReactionRibotypingMicrobiologylaw.inventionRhizoctoniaRhizoctonia solanichemistry.chemical_compoundlawGeneticsTypingDNA FungalMycological Typing TechniquesMolecular BiologyRibosomal DNA[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyPolymerase chain reactionbiologyFungal geneticsfood and beveragesGeneral Medicinebiology.organism_classificationMolecular biologyTerminal restriction fragment length polymorphism[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologychemistryRestriction fragment length polymorphismDNAPolymorphism Restriction Fragment LengthCanadian journal of microbiology
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C4 DNA RFLP reference typing report.

1990

One hundred and three individual DNA samples (including 23 families) were studied at the gene level during the reference typing of the fourth component of human complement at the VIth Complement Genetics Workshop in Mainz (1989). All samples were analyzed with the restriction enzyme Taq I and with two DNA probes recognizing the 5' ends of both C4 genes and the two adjacent 21-hydroxylase genes. This RFLP is informative for the number of C4 genes as well as for their respective gene size. We found a high degree of variation regarding the number of C4 genes, i.e. haplotypes with 1-3 structural C4 genes of 16 or 22 kb size. By correlating these haplotypes to the complotypes obtained by protein…

ImmunologyBiologyMajor Histocompatibility Complexchemistry.chemical_compoundHumansTypingDeoxyribonucleases Type II Site-SpecificGeneAllelesGeneticsModels GeneticHybridization probeHaplotypeGenetic VariationComplement C4HematologyDNARestriction enzymeBlotting SouthernchemistryHaplotypesMultilocus sequence typingSteroid 21-HydroxylaseRestriction fragment length polymorphismDNAPolymorphism Restriction Fragment LengthComplement and inflammation
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Bgl II restriction fragment length polymorphism of human complement C4A gene coincides with BF*F allele of factor B.

1988

ImmunologyImmunogeneticsBiologyComplement factor Bchemistry.chemical_compoundRestriction mapBacterial ProteinsGeneticsHumansAlleleDeoxyribonucleases Type II Site-SpecificGeneAllelesSouthern blotGeneticsRecombination GeneticEnzyme PrecursorsPolymorphism GeneticComplement C4aNucleic Acid HybridizationComplement C4DNA Restriction EnzymesMolecular biologychemistryHaplotypesRestriction fragment length polymorphismDNAPolymorphism Restriction Fragment LengthComplement Factor BImmunogenetics
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IL-6 -174G/C polymorphism and IL-6 serum levels in patients with liver cirrhosis and hepatocellular carcinoma.

2011

Recently, a link between high levels of circulating IL-6 and hepatocellular carcinoma (HCC) has been proposed. In addition, single nucleotide polymorphisms (SNPs) in the promoter region of the IL-6 gene have been reported to be related to several inflammatory-related conditions, including cancer. The purpose of this article is: (1) to evaluate the frequencies of SNPs in the IL-6 promoter region at position -174 and IL-6 serum levels in a group of patients with HCC and underlying liver cirrhosis (LC), and compare them with a group of LC patients without HCC; (2) to determine whether a possible correlation exists between the allelic variations, IL-6 serum levels, and the risk of developing HC…

Liver CirrhosisMaleCirrhosisCarcinoma HepatocellularGenotypeSNPSingle-nucleotide polymorphismEnzyme-Linked Immunosorbent AssayBioinformaticsBiochemistryPolymerase Chain ReactionPolymorphism Single NucleotideGene FrequencyGeneticsmedicineHumansAlleleHCCInterleukin 6Molecular BiologyGeneIL-6biologyInterleukin-6Liver NeoplasmsPromotermedicine.diseaseHepatocellular carcinomabiology.proteinCancer researchIL-6 -174G/C polymorphism liver cirrhosis hepatocellular carcinomaMolecular MedicineFemaleRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthBiotechnology
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Identification of Cpgp40/15 Type Ib as the Predominant Allele in Isolates of Cryptosporidium spp. from a Waterborne Outbreak of Gastroenteritis in So…

2006

ABSTRACT Cryptosporidium sp. isolates from a waterborne outbreak of diarrhea in France were analyzed by PCR-restriction fragment length polymorphism analysis and sequencing of the Cpgp40/15 locus. Ninety-one percent of the isolates were Cryptosporidium hominis type Ib. The results of this study and those of studies of other outbreaks suggest that the type Ib allele is the predominant allele associated with waterborne cryptosporidiosis.

MESH : France/epidemiologyEpidemiologyMESH : polymerase chain reactionMESH : molecular sequence dataProtozoan ProteinsCryptosporidiosisPolymerase Chain Reactionlaw.inventionDisease OutbreaksMESH : Cryptosporidium/geneticsMESH : water/parasitologylaw[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyMESH : gastroenteritis/parasitologyMESH : Polymorphism restriction fragment lengthwaterborne outbreakPolymerase chain reactionbiologyMESH : DNA Protozoan/analysisCryptosporidiumGastroenteritisDiarrheaMESH : Cryptosporidiosis/epidemiologyFrancemedicine.symptomMESH : Cryptosporidium/classificationCryptosporidium hominisMESH : Protozoan proteins/metabolismPolymorphism Restriction Fragment LengthMicrobiology (medical)MESH : Cryptosporidium/isolation&purificationMolecular Sequence DataCryptosporidiumLocus (genetics)MESH : Disease outbreaksMicrobiologyMESH : Cryptosporidiosis/parasitologymedicineAnimalsAlleleGenotyping[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyAllelesMESH : animalsMESH : sequence analysis DNAOutbreakWaterSequence Analysis DNADNA Protozoanbiology.organism_classificationMESH : protozoan proteins/geneticsVirologygenotypingMESH : Gastroenteritis/epidemiologyMESH : Alleles
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Mitochondrial genetic haplogroups and cardiovascular diseases: Data from the Osteoarthritis Initiative

2019

Background Some case-control studies reported that mitochondrial haplogroups could be associated with the onset of cardiovascular diseases (CVD), but the literature regarding this topic is limited. We aimed to investigate whether any mitochondrial haplogroup carried a higher or lower risk of CVD in a large cohort of North American people affected by knee osteoarthritis or at high risk for this condition. Materials and methods A longitudinal cohort study including individuals from the Osteoarthritis Initiative was done. Haplogroups were assigned through a combination of sequencing and PCR-RFLP techniques. All the mitochondrial haplogroups have been named following this nomenclature: HV, JT, …

Male0301 basic medicineHeredityKneesCardiovascular MedicineBiochemistryHaplogroup0302 clinical medicineMedicine and Health SciencesMedicineLongitudinal StudiesProspective StudiesProspective cohort studyMusculoskeletal SystemEnergy-Producing Organelleseducation.field_of_studyMultidisciplinaryQHazard ratioRMiddle AgedOsteoarthritis KneeMitochondrial DNAMitochondriaNucleic acidsGenetic MappingGenes MitochondrialCardiovascular DiseasesResearch Design030220 oncology & carcinogenesisLegsMedicineFemaleCellular Structures and OrganellesAnatomyPolymorphism Restriction Fragment LengthResearch ArticleRiskmedicine.medical_specialtyForms of DNASciencecardiovascuar diseases (CVD)PopulationBioenergeticsResearch and Analysis MethodsLower riskDNA Mitochondrial03 medical and health sciencesRheumatologyInternal medicineOsteoarthritisGeneticsHumansGenetic Predisposition to DiseaseeducationAgedProportional Hazards ModelsEvolutionary BiologyPopulation Biologybusiness.industryProportional hazards modelArthritismitochondrial haplogroupHaplotypeBiology and Life SciencesCell BiologyDNA030104 developmental biologyHaplotypesCase-Control StudiesBody LimbsHaplogroupsbusinessPopulation GeneticsFollow-Up StudiesHuman mitochondrial DNA haplogroupPLOS ONE
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SARS CoV2 infection _The longevity study perspectives

2021

Graphical abstract

MaleAgingssRNA single-stranded RNARFLP restriction fragment length polymorphismHSPs heat shock proteinsReviewPTMs post-translational modificationsSevere Acute Respiratory SyndromeBiochemistryHIV-1 human immunodeficiency virus-1TNF-α tumor necrosis factor-αEC endothelial cells0302 clinical medicineFluAV influenza A virusI insertionMedicineIFN-γ interferon-γDIC disseminated intravascular coagulationPCR Polymerase Chain Reactionmedia_commonAged 80 and overLongevityRBD receptor-binding domainNeurologyLongevity modelMI myocardial infarctionNK natural killerhPIV2 human parainfluenza virus type 2media_common.quotation_subjectResearching genetic basis of resistance and potential pharmacological targetsLongevityDBP diastolic blood pressureNF-Kb nuclear transcription factor kBRANTES regulated upon activation normal T cell expressed and secretedMphi human macrophages03 medical and health sciencesCox 2 cyclooxygenase 2ORF open reading framePT prothrombin timeSettore MED/05 - Patologia ClinicaHumansMolecular BiologyInflammatory genesARDS acute respiratory distress syndromeNO nitric oxideD deletionCpGIs CpG islandsT2DM type 2 diabetes mellitusmedicine.diseaseFDP fibrin degradation products030104 developmental biologySARS CoV2 severe acute respiratory syndrome Coronavirus 2 virusImmunologyBMI body max indexItalian nonagenarians/centenariansRSV respiratory syncytial virusComplication030217 neurology & neurosurgeryMAPK mitogen-activated protein kinaseIP-10 IFN-γ -Inducible Protein 1040301 basic medicineAT1R activity of angiotensin 1 receptorsDCs dentritic cellsSSCP single strand conformation polymorphismACE/DD polymorphism of the angiotensin converting enzymeFGF21 fibroblast growth factor 21TLR4 toll-like receptor 4NAD nicotinamide adenine dinucleotideACE angiotensin-I converting enzymeAT2R activity of angiotensin 2 receptorsCOVID-19 Coronavirus disease 2019Respiratory distressACE2 angiotensin converting enzyme 2MKP-1 mitogen-activated protein kinase phosphatase-1 ()PD protease domainSNP single nucleotide polymorphismEH essential hypertensionTNFR tumor necrosis factor receptorINR international normalized ratio of the prothrombin timePAI-1 plasminogen activator inhibitor-1Ang angiotensinLPS lipopolysaccharideMCP1 monocyte chemoattractant protein-1medicine.symptomaPTT partial thromboplastin timeBiotechnologyDUSP1 dual specificity phosphatase 1Coronavirus disease 2019 (COVID-19)PC prostate cancerRAS renin-angiotensin aldosterone systemCCR5Δ32 genetic variant of chemokine receptorCOVID-19 Researching genetic basis of resistance and potential pharmacological targets Italian nonagenarians/centenarians Longevity modelAsymptomaticSARS-1 severe acute respiratory syndrome virus 1SIRT-1 Sirtuin 1Th1 t-helper lymphocyte type 1Immune systemROS reactive oxygen speciesTGF-β transforming growth factor betaET-1 endothelin-1ComputingMethodologies_COMPUTERGRAPHICSADAM-17 metallopeptidase domain 17business.industrySARS-CoV-2SBP systolic blood pressureCOVID-19HDACs histone deacetylasesComorbidityImmune Systembusiness5-LO lipoxygenase 5Ageing Research Reviews
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Multiple identification of a particular type of hereditary C1q deficiency in the Turkish population: review of the cases and additional genetic and f…

1997

Complete selective deficiencies of the complement component C1q are rare genetic disorders that are associated with recurrent infections and a high prevalence of lupus erythematosus-like symptoms. All C1q deficiencies studied at the genetic level revealed single-base mutations leading to termination codons, frameshifts or amino acid exchanges and these were thought to be responsible for the defects as no other aberrations were found. One particular mutation, leading to a stop codon in the C1qA gene, was first identified in members of a Gypsy family from the Slovak Republic. The same mutation has been found in all cases of C1q deficiency from Turkey that have been investigated. Here we prese…

MaleHeterozygoteSlovakiaTurkish populationRomaTurkeyGenetic counselingMolecular Sequence DataPopulationBiologyPolymerase Chain ReactionGenetic analysisGeneticsHumansPoint MutationAmino Acid SequenceeducationGeneGenetics (clinical)Geneticseducation.field_of_studyBase SequenceComplement C1qImmunologic Deficiency SyndromesHuman geneticsStop codonPedigreeChild PreschoolMutation (genetic algorithm)Codon TerminatorFemalePolymorphism Restriction Fragment LengthHuman Genetics
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Mitochondrial DNA effects on fitness in Drosophila subobscura

2011

We tested different fitness components on a series of conspecific mtDNA haplotypes, detected by RFLPs in Drosophila subobscura. Additionally, haplotype VIII, endemic to the Canary Islands, was tested upon its own native nuclear DNA background and upon that of the rest of mtDNAs tested herein. We found that both nuclear and mitochondrial DNA can have a significant effect upon their hosts' fitness, and that negative selection is one of the mechanisms that can intervene in this species' mtDNA haplotype pattern. We discuss the importance of this mechanism in relation to genetic drift, in the form of periodic population bottlenecks, and how the latter can enhance the former. We also detected a s…

MaleMitochondrial DNALongevityGenetic FitnessBiologyDNA MitochondrialGenetic driftGenetic variationHybrid VigorGeneticsAnimalsSelection GeneticGenetics (clinical)Cell NucleusGeneticsGenetic DriftHaplotypeGenetic VariationDrosophila subobscuraNuclear DNAFertilityHaplotypesSpainEvolutionary biologyMutationOriginal ArticleDrosophilaFemaleGenetic FitnessRestriction fragment length polymorphismWolbachiaHeredity
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