Search results for "Restriction enzyme"

showing 5 items of 45 documents

ABO genotyping by PCR-RFLP and cloning and sequencing

2005

A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications. Four fragments of exon 6 and 7 of the ABO gene were amplified and digested by in total 7 restriction endonucleases. Particular attention was paid to the base changes at nucleotide positions 261(delG), 297, 526, 703, 721, 771, 796 and 1060(delC) in order to distinguish the six common alleles A101, A201, B, O01, O02 and O03. Furthermore, this method also enables determination of…

Sequence analysisBiologyPolymerase Chain ReactionABO Blood-Group Systemlaw.inventionlawABO blood group systemGenotypeHumansCloning MolecularGenotypingAllelesHistory AncientEcology Evolution Behavior and SystematicsPolymerase chain reactionGeneticsReproducibility of ResultsSequence Analysis DNAGeneral MedicineForensic MedicineRestriction enzymePhenotypeAncient DNAArchaeologyBlood StainsPostmortem ChangesAnthropologyDNA Transposable ElementsAnimal Science and ZoologyChromosome DeletionRestriction fragment length polymorphismToothPolymorphism Restriction Fragment LengthAnthropologischer Anzeiger
researchProduct

Genotyping of GII.4 and GIIb norovirus RT-PCR amplicons by RFLP analysis

2007

GII.4 and GIIb/Hilversum norovirus (NoV) strains appear to have a prominent epidemiological role in outbreaks or sporadic cases of human gastroenteritis. Sequence analysis, although laborious, is the reference method used for characterization of noroviruses. In this study a screening test is proposed to characterize GIIb and GII.4 NoVs based on restriction fragment length polymorphism (RFLP) analysis of amplicons obtained from the RNA-dependent RNA polymerase (RdRp) region. Virtual analysis of 793 RdRp sequences of GGI and GGII NoVs, retrieved from GenBank, and representative of global geographical origins on a long-time period, permitted the selection of four restriction enzymes, XmnI, Ahd…

Settore MED/07 - Microbiologia E Microbiologia ClinicaGenotypeSequence analysisvirusesBiologymedicine.disease_causeVirologyGenotypemedicineHumansTypingGenotypingCaliciviridae InfectionsGeneticsReverse Transcriptase Polymerase Chain ReactionNorovirusvirus diseasesInfantVirologyGastroenteritisRestriction enzymeGIIb/Hilversum strain GII.4 genotype Restriction fragment length polymorphism (RFLP)GenBankChild PreschoolNorovirusRNA ViralRestriction fragment length polymorphismPolymorphism Restriction Fragment Length
researchProduct

Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-…

1987

Micrococcal nuclease digestion has been used to investigate some fine details of the chromatin structure of the yeast SUC2 gene for invertase. Precisely positioned nucleosomes have been found on a 2 kb sequence from the 3' non-coding region, and four nucleosomes also seem to occupy fixed positions on the 5' flank. Eleven nucleosomes lie on the coding region, although their positioning is not as precise as in the flanks. When the gene is derepressed, these latter nucleosomes adopt a more open conformation and so do two of the nucleosomes positioned on the 5' flank. A dramatic change occurs in the 3' flank, whose involvement in the structural transitions of chromatin upon gene activation is p…

Regulation of gene expressionGeneticsbiologyGlycoside Hydrolasesbeta-FructofuranosidaseGenes FungalChromosomeDNA Restriction EnzymesSaccharomyces cerevisiaeChromatinChromatinNucleosomesPlasmidGenesGeneticsbiology.proteinNucleosomeCoding regionMicrococcal NucleaseEnzyme RepressionDerepressionMicrococcal nuclease
researchProduct

2001

Eight strains of Taylorella equigenitalis were identified by a polymerase chain reaction using a primer pair specific to the 16S rDNA of T. equigenitalis. These eight strains were chosen because they had previously been shown to represent eight distinct genotypes by pulsed-field gel electrophoresis analysis after separate digestion of the genomic DNA with ApaI or NotI. The eight strains could be classified into six or seven types by random amplified polymorphic DNA analysis using different kinds of primers. Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1500 bp fragments of rDNA amplified by polymerase chain reacti…

Gel electrophoresisGeneticsGeneral VeterinarybiologyGeneral Medicinebiology.organism_classificationMolecular biologyAmplified Ribosomal DNA Restriction AnalysisRestriction enzymeTaylorella equigenitalisCleaved amplified polymorphic sequencePulsed-field gel electrophoresisAmplified fragment length polymorphismRestriction fragment length polymorphismVeterinary Research Communications
researchProduct

Restriction fragment polymorphisms of the HLA-DR, HLA-DQ, and insulin gene regions in IDDM: The GAW5 data

1989

The primary aim of the insulin-dependent diabetes mellitus (IDDM) component of Genetic Analysis Workshop 5 (GAW5) was to collect and analyze new data on DNA polymorphisms closely linked to the HLA-D region and the insulin gene. The probes and restriction enzymes described here were used by all ten participating labs, and the data from Southern blotting were interpreted and reported according to conventions developed for the Workshop. These DNA data on members of 94 families with two or more IDDM sibs constitute the largest such sample available. The data were used in most of the analyses presented at the Workshop meeting, and are available on request.

GeneticsbiologyEpidemiologyHuman leukocyte antigenmedicine.diseaseGenetic analysisRestriction fragmentRestriction enzymeDiabetes mellitusHLA-DQmedicinebiology.proteinHLA-DRGenetics (clinical)Southern blotGenetic Epidemiology
researchProduct