Search results for "Ross"

showing 10 items of 8987 documents

Transcriptomic responses to biotic stresses in Malus x domestica: a meta-analysis study.

2017

AbstractRNA-Seq analysis is a strong tool to gain insight into the molecular responses to biotic stresses in plants. The objective of this work is to identify specific and common molecular responses between different transcriptomic data related to fungi, virus and bacteria attacks in Malus x domestica. We analyzed seven transcriptomic datasets in Malus x domestica divided in responses to fungal pathogens, virus (Apple Stem Grooving Virus) and bacteria (Erwinia amylovora). Data were dissected using an integrated approach of pathway- and gene- set enrichment analysis, Mapman visualization tool, gene ontology analysis and inferred protein-protein interaction network. Our meta-analysis revealed…

0106 biological sciences0301 basic medicineMalusKnowledge BasesArabidopsislcsh:MedicineSecondary MetabolismErwiniaGenes Plant01 natural sciencesArticleTranscriptome03 medical and health sciencesPlant Growth RegulatorsGene Expression Regulation PlantStress PhysiologicalSettore AGR/07 - Genetica AgrariaProtein Interaction Mapslcsh:ScienceSecondary metabolismGeneCrosses GeneticPlant ProteinsGeneticsMultidisciplinarybiologyGene Expression Profilinglcsh:RfungiMalus transcriptomic biotic stressfood and beveragesBiotic stressbiology.organism_classificationSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeGene expression profiling030104 developmental biologyGene OntologyMalustranscriptomic responses biotic stress meta-analysis RNA-seq plantsInactivation Metaboliclcsh:QTranscriptomeApple stem grooving virus010606 plant biology & botanyTranscription FactorsScientific reports
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Gaining Insight into Exclusive and Common Transcriptomic Features Linked with Biotic Stress Responses in Malus

2017

Identifying key information in transcriptomic data is very important, especially when the “omic” study deals with plant responses to stresses in field conditions where a high number of variables and disturbing factors may affect the analysis. In this meta-analysis we collected 12 transcriptomic works in Malus in order to identify which key genes, proteins, gene categories are involved in general plant pathological conditions and those features linked with exclusive biotic stress responses. Those genes that are only related with molecular responses to pathogen attacks and those linked with other plant physiological processes were identified. A pipeline composed by pathway and gene set enrich…

0106 biological sciences0301 basic medicineMalusPlant ScienceComputational biologylcsh:Plant cultureErwinia01 natural sciencesTranscriptometranscriptomics03 medical and health sciencesSettore AGR/07 - Genetica AgrariaHeat shock proteinBotanylcsh:SB1-1110GeneTranscription factorOriginal Researchbiologybiotic stresses; Malus; meta-analysis; protein-protein interaction network; transcriptomicsfood and beveragesBiotic stressbiotic stressesbiology.organism_classificationmeta-analysisCrosstalk (biology)030104 developmental biologyMalusbiotic stresses Malus meta-analysis protein-protein interaction network transcriptomicsprotein-protein interaction network010606 plant biology & botanyFrontiers in Plant Science
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Bacillus thuringiensis Vip3Aa Toxin Resistance in Heliothis virescens (Lepidoptera: Noctuidae)

2017

ABSTRACT Laboratory selection with Vip3Aa of a field-derived population of Heliothis virescens produced >2,040-fold resistance in 12 generations of selection. The Vip3Aa-selected (Vip-Sel)-resistant population showed little cross-resistance to Cry1Ab and no cross-resistance to Cry1Ac. Resistance was unstable after 15 generations without exposure to the toxin. F 1 reciprocal crosses between Vip3Aa-unselected (Vip-Unsel) and Vip-Sel insects indicated a strong paternal influence on the inheritance of resistance. Resistance ranged from almost completely recessive (mean degree of dominance [ h ] = 0.04 if the resistant parent was female) to incompletely dominant (mean h = 0.53 if the resistan…

0106 biological sciences0301 basic medicineMultifactorial Inheritancemedia_common.quotation_subjectPopulationInsectBiology01 natural sciencesApplied Microbiology and BiotechnologyInsecticide ResistanceLepidoptera genitaliaHemolysin Proteins03 medical and health sciencesBacterial ProteinsBacillus thuringiensisInvertebrate MicrobiologyAnimalsSelection GeneticeducationCrosses Geneticmedia_commonGeneticseducation.field_of_studyBacillus thuringiensis ToxinsEcologyHeliothis virescensfungibiology.organism_classificationSurvival AnalysisEndotoxinsLepidoptera010602 entomology030104 developmental biologyCry1AcPaternal InheritanceNoctuidaeBiological AssayPEST analysisFood ScienceBiotechnologyApplied and Environmental Microbiology
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Strength of sexual and postmating prezygotic barriers varies between sympatric populations with different histories and species abundances.

2019

The impact of different reproductive barriers on species or population isolation may vary in different stages of speciation depending on evolutionary forces acting within species and through species' interactions. Genetic incompatibilities between interacting species are expected to reinforce prezygotic barriers in sympatric populations and lead to cascade reinforcement between conspecific populations living within and outside the areas of sympatry. We tested these predictions and studied whether and how the strength and target of reinforcement between Drosophila montana and Drosophila flavomontana vary between sympatric populations with different histories and species abundances. All barri…

0106 biological sciences0301 basic medicineSympatryReproductive IsolationReciprocal crossmedia_common.quotation_subjectPopulationZoologyBiology010603 evolutionary biology01 natural sciences03 medical and health sciencesSexual Behavior AnimalGeneticsAnimalseducationDrosophilaEcology Evolution Behavior and Systematicsmedia_commonPopulation DensityDrosophila montanaeducation.field_of_studyReproductive isolationbiology.organism_classificationBiological EvolutionSpeciationSympatry030104 developmental biologySympatric speciationDrosophilaGeneral Agricultural and Biological SciencesEvolution; international journal of organic evolution
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PERMANENT GENETIC RESOURCES: Polymorphic microsatellite loci and interspecific cross-amplification in the parasitoid wasps Megastigmus stigmatizans a…

2008

We isolated and characterized 19 polymorphic microsatellite loci in the congeneric parasitoid wasps Megastigmus stigmatizans and Megastigmus dorsalis associated with cynipid oak galls. Loci isolated from species-specific libraries showed extensive cross-amplification, resulting in a total of 15 polymorphic loci for M. stigmatizans and 13 for M. dorsalis.

0106 biological sciences0303 health sciencesfood.ingredientbiologyfungiZoologyInterspecific competitionbiology.organism_classification010603 evolutionary biology01 natural sciences3. Good healthParasitoidMegastigmus stigmatizans03 medical and health sciencesMegastigmus dorsalisfoodMegastigmusBotanyGeneticsMicrosatellitePolymorphic locusCross amplificationEcology Evolution Behavior and Systematics030304 developmental biologyBiotechnologyMolecular Ecology Resources
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Monitoring of transglutaminase crosslinking reaction by 1H NMR spectroscopy on model substrates

2015

International audience; A new method based on 1H NMR spectroscopy was developed for monitoring transglutaminase crosslinking reaction with model molecules (CBZ-Gln-Gly and N-α-acetyl-lysine). The transglutaminase reaction led to the appearance of new resonances on NMR spectrum as well as significant decrease in others. The new observed resonances, originated from newly formed ɛ-(γ-glutamyl)lysine isopeptide bonds, evidence the enzymatic reaction and allow to quantify the ɛ-(γ-glutamyl)lysine fragment. Moreover, the decrease in resonance intensity, originated from lysine, permit to determine the crosslinking degree. These results obtained by 1H NMR spectroscopy can be used as an alternative …

0106 biological sciences1h nmr spectroscopyTissue transglutaminaseLysineCrosslinking degreePhotochemistrycomplex mixtures01 natural sciences03 medical and health sciencesModel substratesɛ-(γ-glutamyl)-lysineColloid and Surface ChemistryLiquid chromatography–mass spectrometry010608 biotechnologyOrganic chemistryMolecule[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology030304 developmental biologyAlternative methods0303 health sciencesbiologyChemistryResonanceNuclear magnetic resonance spectroscopyMicrobial transglutaminasebiology.proteinColloids and Surfaces A: Physicochemical and Engineering Aspects
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One taxon does not fit all: Herb-layer diversity and stand structural complexity are weak predictors of biodiversity in Fagus sylvatica forests

2016

Abstract Since adequate information on the distribution of biodiversity is hardly achievable, biodiversity indicators are necessary to support the management of ecosystems. These surrogates assume that either some habitat features, or the biodiversity patterns observed in a well-known taxon, can be used as a proxy of the diversity of one or more target taxa. Nevertheless, at least for certain taxa, the validity of this assumption has not yet been sufficiently demonstrated. We investigated the effectiveness of both a habitat- and a taxa-based surrogate in six European beech forests in the Apennines. Particularly, we tested: (1) whether the stand structural complexity and the herb-layer speci…

0106 biological sciencesApennineApennines010504 meteorology & atmospheric sciencesBiodiversityGeneral Decision SciencesComplementarity010603 evolutionary biology01 natural sciencesEuropean beech forestsHabitat-based surrogatesLichenBeechEcology Evolution Behavior and Systematics0105 earth and related environmental sciencesDecision Sciences(all)EcologybiologyEcologySpecies diversityBody size and species richnessCross-taxonbiology.organism_classificationEuropean beech forestTaxonHabitatHabitat-based surrogateSpecies richnessSpecies-based surrogatesApennines; Cross-taxon; Complementarity; European beech forests; Habitat-based surrogates; Species-based surrogatesEcological Indicators
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The shell matrix of the pulmonate land snail Helix aspersa maxima.

2012

12 pages; International audience; In mollusks, the shell mineralization process is controlled by an array of proteins, glycoproteins and polysaccharides that collectively constitute the shell matrix. In spite of numerous researches, the shell protein content of a limited number of model species has been investigated. This paper presents biochemical data on the common edible land snail Helix aspersa maxima, a model organism for ecotoxicological purposes, which has however been poorly investigated from a biomineralization viewpoint. The shell matrix of this species was extracted and analyzed biochemically for functional in vitro inhibition assay, for amino acid and monosaccharides composition…

0106 biological sciencesBiomineralizationPulmonate snailPhysiology01 natural sciencesBiochemistryMineralization (biology)chemistry.chemical_compoundX-Ray DiffractionTandem Mass SpectrometryElectrophoresis Gel Two-DimensionalHaliotisAmino AcidsComputingMilieux_MISCELLANEOUSchemistry.chemical_classification0303 health sciencesEcologyMonosaccharidesLand snailImmunogold labellingImmunohistochemistryAmino acidBiochemistryElectrophoresis Polyacrylamide GelTerrestrial snail ; biomineralization ; shell ; aragonite ; crossed-lamellar ; protein ; immunogold ; gel electrophoresisFrancefood.ingredientBiology010603 evolutionary biologyCalcium Carbonate03 medical and health sciencesfoodSpecies SpecificityAnimal ShellsShellAnimals14. Life underwater[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsMolecular Biology030304 developmental biologyHelix SnailsProteinsCrossed-lamellarbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/BiomaterialsGel electrophoresis[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate ZoologyCalcium carbonatechemistryMicroscopy Electron ScanningBiomineralizationPinctadaComparative biochemistry and physiology. Part B, Biochemistrymolecular biology
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gllvm: Fast analysis of multivariate abundance data with generalized linear latent variable models inr

2019

The work of J.N. was supported by the Wihuri Foundation. The work of S.T. was supported by the CRoNoS COST Action IC1408.F.K.C.H. was also supported by an ANU cross disciplinary grant.

0106 biological sciencesClustering high-dimensional dataMultivariate statisticsMultivariate analysisCross disciplinary010604 marine biology & hydrobiologyEcological ModelingMaximum likelihoodLatent variable010603 evolutionary biology01 natural sciencesAbundance (ecology)StatisticsCost actionEcology Evolution Behavior and SystematicsMathematicsMethods in Ecology and Evolution
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Introducing anatomical techniques to subfossil wood

2018

Abstract Successful cross-dating of subfossil wood, ideally in combination with precise information on germination and dieback, requires the accurate detection of tree-ring width (TRW) boundaries along continuous measurement tracks from pith to bark. However, wood decay and the mechanical deformation of cells often challenge the dendrochronological analysis and subsequent paleoclimatic and environmental interpretations. Here, we show that wood anatomical techniques can improve the assessment of heavily degraded and/or deformed material. We apply state-of-the-art sample preparation, thin sectioning and double-staining to a unique collection of Late Glacial pines that were growing ∼13,000 yea…

0106 biological sciencesContinuous measurementSubfossilCross-dating; Cell deformation; Subfossil wood; Thin sectioning; Wood anatomy; wood decay010504 meteorology & atmospheric sciencesEcologyPlant Science15. Life on land01 natural sciencescomplex mixturesPaleontologyPithThin SectioningGeology010606 plant biology & botany0105 earth and related environmental sciences
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