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showing 10 items of 16104 documents

Transcriptome analysis of the Populus trichocarpa–Rhizophagus irregularis Mycorrhizal Symbiosis: Regulation of Plant and Fungal Transportomes under N…

2017

Nutrient transfer is a key feature of the arbuscular mycorrhizal (AM) symbiosis. Valuable mineral nutrients are transferred from the AM fungus to the plant, increasing its fitness and productivity, and, in exchange, the AM fungus receives carbohydrates as an energy source from the plant. Here, we analyzed the transcriptome of the Populus trichocarpa-Rhizophagus irregularis symbiosis using RNA-sequencing of non-mycorrhizal or mycorrhizal fine roots, with a focus on the effect of nitrogen (N) starvation. In R. irregularis, we identified 1,015 differentially expressed genes, whereby N starvation led to a general induction of gene expression. Genes of the functional classes of cell growth, memb…

0106 biological sciences0301 basic medicineRhizophagus irregularisMICROBE INTERACTIONSPhysiologyarbuscule[SDV]Life Sciences [q-bio]racine finePlant Science01 natural sciencesnitrogenTranscriptomeGene Expression Regulation PlantMycorrhizaeLOTUS-JAPONICUSGLOMUS-INTRARADICESPlant ProteinsGENE-EXPRESSION2. Zero hungerazotePHOSPHATE TRANSPORTERAMMONIUM TRANSPORTERSorgan transplantationGeneral Medicinefood shortageMedicago truncatulaArbuscular mycorrhizasymbiose mycorhiziennePopulusfamineEnergy sourceARBUSCULAR MYCORRHIZABiologySULFUR STARVATION03 medical and health sciencesPHOSPHORUS ACQUISITIONSymbiosistransport de nutrimentsBotanySymbiosisGene Expression Profilingblack cottonwoodCell Biologybiology.organism_classificationMEDICAGO-TRUNCATULATransplantationpopulus trichocarpa030104 developmental biologyMembrane biogenesis010606 plant biology & botanytransplantation
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Low but contrasting neutral genetic differentiation shaped by winter temperature in European great tits.

2016

Gene flow is usually thought to reduce genetic divergence and impede local adaptation by homogenising gene pools between populations. However, evidence for local adaptation and phenotypic differentiation in highly mobile species, experiencing high levels of gene flow, is emerging. Assessing population genetic structure at different spatial scales is thus a crucial step towards understanding mechanisms underlying intraspecific differentiation and diversification. Here, we studied the population genetic structure of a highly mobile species - the great tit Parus major - at different spatial scales. We analysed 884 individuals from 30 sites across Europe including 10 close-by sites (< 50 km), u…

0106 biological sciences0301 basic medicineSELECTIONZOOLOGIA[SDV]Life Sciences [q-bio]FLOWSOFTWARE01 natural sciencesmicrosatellitesBehavioral EcologyLOCAL ADAPTATIONParus majorComputingMilieux_MISCELLANEOUSeducation.field_of_studyLatitudeCLIMATE-CHANGEEcologyIsolation-by-distancelatitudePE&RCGedragsecologieWILD BIRD POPULATIONinternationalGenetic structureGene poolwinter severityPopulationAnimal Breeding and GenomicsBiologyPARUS-MAJOR010603 evolutionary biology03 medical and health sciencesPopulation genetic structureFokkerij en GenomicaMicrosatelliteseducationBiologyEcology Evolution Behavior and SystematicsLocal adaptationIsolation by distanceisolation-by-distance[SDV.GEN]Life Sciences [q-bio]/GeneticsGenetic diversityF-statisticsGenetic divergenceWinter severity030104 developmental biologyPARTIAL MIGRATIONF-statisticsNATAL DISPERSALRE-IMPLEMENTATIONWIAS570 Life sciences; biologyta1181Biological Journal of the Linnean Society
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Molecule structural factors influencing the loading of flavoring compounds in a natural-preformed capsule: Yeast cells

2016

International audience; Yeast cells are efficient microcapsules for the encapsulation of flavoring compounds. However, as they are preformed capsules, they have to be loaded with the active. Encapsulation efficiency is to a certain level correlated with LogP. In this study, the effect of structural factors on the encapsulation of amphiphilic flavors was investigated. Homological series of carboxylic acids, ethyl esters, lactones, alcohols and ketones were encapsulated into the yeast Yarrowia lipolytica. Although, in a single homological series, the length of the molecule and thus the LogP were correlated with encapsulation efficiency (EY%), big differences were observable between series. Fo…

0106 biological sciences0301 basic medicineSaccharomyces cerevisiaeCapsulesSaccharomyces-cerevisiae01 natural sciencesHexanalYeast cellDiffusion03 medical and health scienceschemistry.chemical_compoundColloid and Surface ChemistryFlavorsYeasts010608 biotechnologyAmphiphileMechanismsOrganic chemistryMoleculePhysical and Theoretical ChemistryMicroencapsulationHexanoic acidMolecular StructurebiologyToxicityMembrane[ SDV.IDA ] Life Sciences [q-bio]/Food engineeringYarrowiaSurfaces and InterfacesGeneral Medicinebiology.organism_classificationFood ingredientsOrganic-solventsYeastFlavoring AgentsMicrocapsules030104 developmental biologyMembranechemistryFunctional groupsEncapsulationDeliveryMolecule structureBiotechnology
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Modelling nonlinear dynamics of Crassulacean acid metabolism productivity and water use for global predictions

2021

Crassulacean acid metabolism (CAM) crops are important agricultural commodities in water-limited environments across the globe, yet modeling of CAM productivity lacks the sophistication of widely used C3 and C4 crop models, in part due to the complex responses of the CAM cycle to environmental conditions. This work builds on recent advances in CAM modeling to provide a framework for estimating CAM biomass yield and water use efficiency from basic principles. These advances, which integrate the CAM circadian rhythm with established models of carbon fixation, stomatal conductance, and the soil-plant-atmosphere continuum, are coupled to models of light attenuation, plant respiration, and bioma…

0106 biological sciences0301 basic medicineStomatal conductanceOpuntia ficus-indicawater use efficiencyPhysiologyPlant ScienceAgricultural engineering01 natural sciencescarbon assimilation03 medical and health sciencesAgaveBiomassWater-use efficiencyPhotosynthesisProductivityTranspirationBiomass (ecology)OpuntiaWaterPlant TranspirationCarbonAgave tequilanaSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree030104 developmental biologyNonlinear DynamicsCrassulacean acid metabolismbiomaCrassulacean acid metabolismEnvironmental scienceBiomass partitioningWater use010606 plant biology & botany
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Molecular Responses to Small Regulating Molecules against Huanglongbing Disease

2016

Huanglongbing (HLB; citrus greening) is the most devastating disease of citrus worldwide. No cure is yet available for this disease and infected trees generally decline after several months. Disease management depends on early detection of symptoms and chemical control of insect vectors. In this work, different combinations of organic compounds were tested for the ability to modulate citrus molecular responses to HLB disease beneficially. Three small-molecule regulating compounds were tested: 1) L-arginine, 2) 6-benzyl-adenine combined with gibberellins, and 3) sucrose combined with atrazine. Each treatment contained K-phite mineral solution and was tested at two different concentrations. T…

0106 biological sciences0301 basic medicineSucroseLeavesCitruslcsh:MedicineGene ExpressionSecondary MetabolismPlant ScienceDisaccharidesBiochemistry01 natural sciencesStarchesGene Expression Regulation PlantINFECTIONMedicine and Health SciencesInnatePlant HormonesAmino Acidslcsh:ScienceImmune ResponseGENE-EXPRESSIONMultidisciplinaryNONHOST RESISTANCEbiologyOrganic CompoundsPlant BiochemistryPlant AnatomyChemistryPhenotypeBiochemistryDEFENSE RESPONSESCANDIDATUS-LIBERIBACTER-ASIATICUS; ARABIDOPSIS-THALIANA; NONHOST RESISTANCE; DEFENSE RESPONSES; CITRUS-SINENSIS; GENE-EXPRESSION; INFECTION; PLANTS; IDENTIFICATION; TRANSCRIPTOMEPhysical SciencesHost-Pathogen InteractionsCarbohydrate MetabolismSucrose synthaseAtrazineGibberellinBasic Amino AcidsStarch synthaseSystemic acquired resistanceResearch ArticleCITRUS-SINENSISGeneral Science & TechnologyPhysiologicalImmunologyCarbohydratesCarbohydrate metabolismStressArginine03 medical and health sciencesStress PhysiologicalSettore AGR/07 - Genetica AgrariaGeneticsPLANTSTRANSCRIPTOMESecondary metabolismGenePlant DiseasesIDENTIFICATIONGene Expression Profilinglcsh:ROrganic ChemistryImmunityChemical CompoundsBiology and Life SciencesProteinsPlantBiotic stressCANDIDATUS-LIBERIBACTER-ASIATICUSHormonesGibberellinsImmunity InnateMetabolism030104 developmental biologyGene Expression RegulationARABIDOPSIS-THALIANAbiology.proteinlcsh:Q010606 plant biology & botanyPLOS ONE
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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UV-screening and springtime recovery of photosynthetic capacity in leaves of Vaccinium vitis-idaea above and below the snow pack

2019

International audience; Evergreen plants in boreal biomes undergo seasonal hardening and dehardening adjusting their photosynthetic capacity and photoprotection; acclimating to seasonal changes in temperature and irradiance. Leaf epidermal ultraviolet (UV)-screening by flavonols responds to solar radiation, perceived in part through increased ultraviolet-B (UV-B) radiation, and is a candidate trait to provide cross-photoprotection. At Hyytiälä Forestry Station, central Finland, we examined whether the accumulation of flavonols was higher in leaves of Vaccinium vitis-idaea L. growing above the snowpack compared with those below the snowpack. We found that leaves exposed to colder temperature…

0106 biological sciences0301 basic medicineTime FactorsPhotoinhibitionBOREALPhysiologyPlant ScienceForests01 natural sciencesPlant EpidermisAnthocyaninsSoilFlavonolsLOW-TEMPERATURESnowPhotosynthesis1183 Plant biology microbiology virologychemistry.chemical_classificationspring dehardening.CLIMATE-CHANGEbiologyChemistryTemperatureUnderstoreyHorticultureLIGHTSeasonsVacciniumUltraviolet RaysGrowing seasonPhotosynthesisDWARF SHRUB03 medical and health sciencesLEAFPHOTOSYSTEM-IIGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyVaccinium vitis-idaeaFlavonoidsSpring dehardeningPhotoprotectionSpectral qualityPhotosystem II Protein ComplexPigments Biological15. Life on landEvergreenbiology.organism_classificationPhotosynthetic capacitySUB-ARCTIC HEATHPlant Leaves030104 developmental biology13. Climate actionPhotoprotectionWINTERB RADIATIONArctic browning010606 plant biology & botany
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NO Signalling in Plant Immunity

2016

The importance of nitric oxide (NO) in innate and adaptive immunity in mammals is well recognised. NO exerts antimicrobial properties against invaders but also displays immunoregulatory functions in which S-nitrosylation represents a signalling process of major importance. Over the last two decades, a growing body of evidence suggests that NO is also a major component of plant immunity. Our understanding of its role in plant defence has been enriched by the identification and functional analysis of S-nitrosylated proteins. The recent identification of new S-nitrosylated proteins including the chaperone-like enzyme cell division cycle 48 (CDC48), histone deacetylases (HDACs) and calmodulin (…

0106 biological sciences0301 basic medicinebiologyCalmodulinPlant ImmunityS-NitrosylationAcquired immune system01 natural sciencesCell biology03 medical and health sciences030104 developmental biologyHistoneSignallingbiology.proteinEpigenetics010606 plant biology & botanyCalcium signaling
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Karyotype variability and inter-population genomic differences in freshwater ostracods (Crustacea) showing geographical parthenogenesis

2018

Transitions from sexual to asexual reproduction are often associated with polyploidy and increased chromosomal plasticity in asexuals. We investigated chromosomes in the freshwater ostracod species Eucypris virens (Jurine, 1820), where sexual, asexual and mixed populations can be found. Our initial karyotyping of multiple populations from Europe and North Africa, both sexual and asexual, revealed a striking variability in chromosome numbers. This would suggest that chromosomal changes are likely to be accelerated in asexuals because the constraints of meiosis are removed. Hence, we employed comparative genomic hybridization (CGH) within and among sexual and asexual populations to get insigh…

0106 biological sciences0301 basic medicinefreshwater ostracodslcsh:QH426-470PopulationDIVERSITYcomparative genomic hybridizationPOLYPLOIDYAsexual reproductionGRASSHOPPER EYPREPOCNEMIS-PLORANSchromosome numbersB-CHROMOSOME POLYMORPHISMBiologyASEXUAL REPRODUCTION010603 evolutionary biology01 natural sciencesArticleAsexuality03 medical and health sciencesNORTH-AFRICAACRIDIDAEGeneticsCopy-number variationeducationRibosomal DNAGenetics (clinical)education.field_of_studygeographical parthenogenesisBiology and Life SciencesParthenogenesisreproductive modesDNAfreshwater ostracods; asexuality; reproductive modes; geographical parthenogenesis; comparative genomic hybridization; chromosome numbers; karyotypekaryotypeORTHOPTERAlcsh:Genetics030104 developmental biologyEvolutionary biologyEarth and Environmental SciencesPHASMATODEAPloidyasexualityComparative genomic hybridization
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Genomic stability through time despite decades of exploitation in cod on both sides of the Atlantic

2021

Significance Both theory and experiments suggest that fishing can drive the evolution of an earlier maturation age. However, determining whether changes in the wild are the result of fisheries-induced evolution has been difficult. Temporal, genome-wide datasets can directly reveal responses to selection. Here, we investigate the genomes of two wild Atlantic cod populations from samples that pre- and postdate periods of intensive fishing. Although phenotypic changes suggest fisheries-induced evolution, we do not find evidence for any strong genomic change or loss of genetic diversity. While evolution could have occurred through undetectable frequency changes at many loci, the irreversible lo…

0106 biological sciences0301 basic medicinepopulation genomicsFishing430010603 evolutionary biology01 natural sciencesGenomeGenomic InstabilityPopulation genomicsEvolution Molecular03 medical and health sciencesselective sweepsEffective population sizeGadusAnimalsBiomassAtlantic OceanGenetic diversityMultidisciplinaryPolymorphism GeneticbiologyPopulation Biologyfisheries-induced evolutiongenetic diversityBiological Sciencesbiology.organism_classification030104 developmental biologyArcticGadus morhuaEvolutionary biologysense organsAtlantic codhistorical DNA
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