Search results for "S-alleles"
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Identification of (In)Compatible S-genotypes and Molecular Characterisation of Italian Sweet Cherry Cultivars.
2017
Italy is the first sweet cherry producer in Europe. Its rich germplasm of local varieties has not been fully characterised or exploited in breeding programmes. Sweet cherry is a self-incompatible species; this trait is controlled by a gametophytically expressed multi-allelic (S) locus. The knowledge of sweet cherry S-alleles and cross-incompatibility groups is important for growers and breeders for choosing appropriate pollinators in the orchard and planning crosses. In this work, we analysed 94 sweet cherry cultivars native of large part of the Italian regions where cherry is grown. The microsatellite primer pair set and the reference cultivars agreed for cherry by the Prunus Working Group…
Identification of self(in)compatibility genotypes and microsatellite marker based fingerprinting of traditional italian sweet cherry accessions
2012
Italy is one of the main European sweet cherry producers and it has a rich germplasm, including many minor local varieties that have not been well studied or used in breeding programmes. Sweet cherry is self-incompatible, with few exceptions; its incompatibility is controlled by a multi-allelic S locus, which is gametophytically expressed. The knowledge of S-alleles and cross-incompatibility groups of sweet cherry genotypes and cultivars is important for growers and breeders. In this work we analysed 48 traditional sweet cherry varieties and cultivars, mainly from Calabria and Emilia Romagna regions, together with eight Sicilian cultivars and the standard set of reference genotypes proposed…
S-genotype identification, genetic diversity and structure analysis of Italian sweet cherry germplasm
2017
In this study, 186 local sweet cherry accessions from 12 Italian regions, plus eight reference accessions, were analysed for the first time, using 13 microsatellite markers. Moreover, their S-incompatibility genotypes were identified with consensus primers for the S-RNase and SFB genes. A total of 161 unique genotypes were found; 18 groups of synonyms, along with the discovery of cases of misidentification. The average number of alleles per locus was 9.7, the mean expected heterozygosity (He) was 0.63, the mean observed heterozygosity (Ho) was 0.65 and the mean polymorphic information content (PIC) was 0.58. The structure analysis revealed the presence of six populations, which reflected in…