Search results for "SAC"

showing 10 items of 3337 documents

Unexpected subcellular distribution of a specific isoform of the Coxsackie and adenovirus receptor, CAR-SIV, in human pancreatic beta cells

2018

Aims/hypothesis: The Coxsackie and adenovirus receptor (CAR) is a transmembrane cell-adhesion protein that serves as an entry receptor for enteroviruses and may be essential for their ability to infect cells. Since enteroviral infection of beta cells has been implicated as a factor that could contribute to the development of type 1 diabetes, it is often assumed that CAR is displayed on the surface of human beta cells. However, CAR exists as multiple isoforms and it is not known whether all isoforms subserve similar physiological functions. In the present study, we have determined the profile of CAR isoforms present in human beta cells and monitored the subcellular localisation of the princi…

0301 basic medicineMaleviruksetEndocrinology Diabetes and MetabolismInsulin-Secreting CellsProtein IsoformsReceptorChildProinsulinEnterovirusMicroscopy ConfocalChemistryNuclear ProteinsImmunogold labellingMiddle AgedFlow CytometryImmunohistochemistryTransmembrane protein3. Good healthCell biologyEndocrinologieenteroviruksetMédecine interneProtein interacting with C-kinase 1 (PICK1)medicine.anatomical_structureChild PreschoolCoxsackievirus BFemalePancreasPICK1Gene isoformBeta cells; Coxsackie and adenovirus receptor; Coxsackievirus B; Enterovirus; Insulin granule; Pancreas; Protein interacting with C-kinase 1 (PICK1)AdultCoxsackie and Adenovirus Receptor-Like Membrane ProteinAdolescentImmunoprecipitationBlotting WesterninsuliiniArticle03 medical and health sciencesYoung AdultMétabolismeInternal MedicinemedicineHumansImmunoprecipitationPancreasCoxsackie and adenovirus receptorInsulin granuleDiabétologieBeta cellshaima030104 developmental biologyDiabetes Mellitus Type 1Carrier ProteinsDiabetologia
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Sequential cleavage of the proteins encoded by HNOT/ALG3, the human counterpart of the Drosophila NOT and yeast ALG3 gene, results in products acting…

2017

This study provides first insights into the biosynthesis, structure, biochemistry and complex processing of the proteins encoded by hNOT/ALG3, the human counterpart of the Drosophila Neighbour of TID (NOT) and the yeast asparagine linked glycosylation 3 gene (ALG3), which encodes a mannosyltransferase. Unambiguous evidence that both the fly and human proteins act as mannosyltransferases has not been provided yet. Previously, we showed that hNOT/ALG3 encodes two alternatively spliced main transcripts, hNOT-1/ALG3-1 and hNOT-4/ALG3-4, and their 15 truncated derivatives that lack diverse sets of exons and/or carry point mutations that result in premature termination codons. Here we show that t…

0301 basic medicineMannosyltransferaseGlycosylationSaccharomyces cerevisiae ProteinsGlycosylationProtein ConformationRNA SplicingSaccharomyces cerevisiaeBiologyMannosyltransferases03 medical and health scienceschemistry.chemical_compoundExonNuclear Receptor Subfamily 4 Group A Member 2GeneticsAnimalsHumansAmino Acid SequenceAsparagineMolecular BiologyGeneGenetics (clinical)Cellular compartmentPoint mutationComputational BiologyMembrane ProteinsExonsGeneral MedicineCell biologyAlternative Splicing030104 developmental biologychemistryCodon NonsenseDrosophilaCytokinesisHuman Molecular Genetics
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Search for a Minimal Set of Parameters by Assessing the Total Optimization Potential for a Dynamic Model of a Biochemical Network.

2017

Selecting an efficient small set of adjustable parameters to improve metabolic features of an organism is important for a reduction of implementation costs and risks of unpredicted side effects. In practice, to avoid the analysis of a huge combinatorial space for the possible sets of adjustable parameters, experience-, and intuition-based subsets of parameters are often chosen, possibly leaving some interesting counter-intuitive combinations of parameters unrevealed. The combinatorial scan of possible adjustable parameter combinations at the model optimization level is possible; however, the number of analyzed combinations is still limited. The total optimization potential (TOP) approach is…

0301 basic medicineMathematical optimizationLinear programmingApplied Mathematics0206 medical engineeringComputational Biology02 engineering and technologySaccharomyces cerevisiaeModels BiologicalSmall setBiochemical networkEnzymes03 medical and health sciences030104 developmental biologyFermentationGeneticsComputer SimulationMETABOLIC FEATURESGlycolysis020602 bioinformaticsMetabolic Networks and PathwaysBiotechnologyMathematicsIntuitionIEEE/ACM transactions on computational biology and bioinformatics
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Differential Contribution of the Parental Genomes to a S. cerevisiae × S. uvarum Hybrid, Inferred by Phenomic, Genomic, and Transcriptomic Analyses, …

2020

In European regions of cold climate, S. uvarum can replace S. cerevisiae in wine fermentations performed at low temperatures. S. uvarum is a cryotolerant yeast that produces more glycerol, less acetic acid and exhibits a better aroma profile. However, this species exhibits a poor ethanol tolerance compared with S. cerevisiae. In the present study, we obtained by rare mating (non-GMO strategy), and a subsequent sporulation, an interspecific S. cerevisiae × S. uvarum spore-derivative hybrid that improves or maintains a combination of parental traits of interest for the wine industry, such as good fermentation performance, increased ethanol tolerance, and high glycerol and aroma productions. G…

0301 basic medicineMating typeHistologylcsh:BiotechnologySaccharomyces cerevisiaeBiomedical EngineeringBioengineeringLocus (genetics)Ethanol tolerance02 engineering and technologySaccharomyces cerevisiaeBiologyGenome sequencingGenome03 medical and health scienceslcsh:TP248.13-248.65Artificial hybridWine fermentationHybridFermentation in winemakingGeneticsfungifood and beverages021001 nanoscience & nanotechnologybiology.organism_classificationYeastethanol tolerancegenome sequencing030104 developmental biologyS. uvarumwine fermentationartificial hybridRNA-seqPloidy0210 nano-technologyBiotechnologyFrontiers in Bioengineering and Biotechnology
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Native Vineyard Non-Saccharomyces Yeasts Used for Biological Control of Botrytis cinerea in Stored Table Grape

2021

Postharvest spoilage fungi, such as Botrytis cinerea, are considered the main cause of losses of fresh fruit quality and vegetables during storage, distribution, and consumption. The current control strategy is the use of SO2 generator pads whose application is now largely under observation. A high quantity of SO2 can be deleterious for fresh fruits and vegetables and it is not allowed in organic agriculture. For this reason, great attention has been recently focused on identifying Biological Control Agents (BCA) to implement biological approaches devoid of chemicals. In this direction, we carried out our study in isolating five different non-Saccharomyces yeast strains from local vineyards…

0301 basic medicineMicrobiology (medical)030106 microbiologyFood spoilageCold storagebiological controlyeastsMicrobiologySaccharomycesArticle040501 horticulture03 medical and health sciencesBotrytis cinereaLachancea thermotoleransVirologyFood sciencePectinaselcsh:QH301-705.5Botrytis cinereabiologyChemistry04 agricultural and veterinary sciencesbiology.organism_classificationMetschnikowia pulcherrimaYeastlcsh:Biology (General)Postharvest0405 other agricultural sciencestable grapeMetschnikowia pulcherrimaMicroorganisms
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Effect of temperature on the prevalence of Saccharomyces non-cerevisiae species against a S. cerevisiae wine strain in wine fermentation: competition…

2017

Saccharomyces cerevisiae is the main microorganism responsible for the fermentation of wine. Nevertheless, in the last years wineries are facing new challenges due to current market demands and climate change effects on the wine quality. New yeast starters formed by non-conventional Saccharomyces species (such as S. uvarum or S. kudriavzevii) or their hybrids (S. cerevisiae x S. uvarum and S. cerevisiae x S. kudriavzevii) can contribute to solve some of these challenges. They exhibit good fermentative capabilities at low temperatures, producing wines with lower alcohol and higher glycerol amounts. However, S. cerevisiae can competitively displace other yeast species from wine fermentations,…

0301 basic medicineMicrobiology (medical)030106 microbiologySaccharomyces cerevisiaeSaccharomyces speciesMicrobiologyParadoxusSaccharomycesAliments Microbiologia03 medical and health sciencesBotanyWine fermentationFitnessFermentacióFood scienceWineFermentation in winemakingTemperaturesbiologyCompetitionWine compositiontemperaturefood and beveragesbiology.organism_classificationYeastYeast in winemakingViniculturaFermentation
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Traditional Norwegian Kveik Are a Genetically Distinct Group of Domesticated Saccharomyces cerevisiae Brewing Yeasts

2018

The widespread production of fermented food and beverages has resulted in the domestication of Saccharomyces cerevisiae yeasts specifically adapted to beer production. While there is evidence beer yeast domestication was accelerated by industrialization of beer, there also exists a farmhouse brewing culture in western Norway which has passed down yeasts referred to as kveik for generations. This practice has resulted in ale yeasts which are typically highly flocculant, phenolic off flavor negative (POF-), and exhibit a high rate of fermentation, similar to previously characterized lineages of domesticated yeast. Additionally, kveik yeasts are reportedly high-temperature tolerant, likely due…

0301 basic medicineMicrobiology (medical)030106 microbiologySaccharomyces cerevisiaelcsh:QR1-502ta3111MicrobiologySaccharomyceslcsh:MicrobiologyDomestication03 medical and health sciencesSaccharomycesDomesticationFermentation in food processingWhole genome sequencingGeneticsbiologybusiness.industryta1183ta1182food and beveragesBrewingbiology.organism_classificationYeastYeast030104 developmental biologyAleKveikFermentationBrewingFermentationbusinessFrontiers in Microbiology
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In Vitro Antimicrobial Activity of Frankincense Oils from Boswellia sacra Grown in Different Locations of the Dhofar Region (Oman)

2020

Frankincense essential oils from Boswellia sacra have been commonly used to treat microbial infections from as early as the 11th century. The main feature of the plant is its gum resin, from which it is possible to obtain essential oils. In the present study, we focused on the comparative study of the oils extracted from the resins of three different Boswellia sacra cultivars (Najdi, Sahli and Houjri). From each of frankincense resin three successive essential oil samples (Grade 1, Grade 2, Grade 3) were obtained. Houjri gum resin gave the lowest percentage (5%) of total essential oil content but showed the maximum number of volatile components in all three grades. Najdi Grade 2 essential o…

0301 basic medicineMicrobiology (medical)Candida albicanStaphylococcus aureusmedicine.disease_causeSettore BIO/19 - Microbiologia Generale01 natural sciencesBiochemistryMicrobiologylaw.invention03 medical and health sciencesPropionibacterium acnesMinimum inhibitory concentrationlawPropionibacterium acneCandida albicansmedicinePharmacology (medical)Propionibacterium acnesGeneral Pharmacology Toxicology and PharmaceuticsCandida albicansEssential oilGC/MS analysiBoswellia sacraantimicrobial activitybiologyTraditional medicine010405 organic chemistrylcsh:RM1-950Malassezia furfurFrankincensebiology.organism_classificationAntimicrobial0104 chemical sciencesBoswellia sacralcsh:Therapeutics. Pharmacology030104 developmental biologyInfectious DiseasesStaphylococcus aureusPseudomonas aeruginosaStaphylococcus aureuGC/MS analysisfrankincense essential oilAntibiotics
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Persistence of Two Non-Saccharomyces Yeasts (Hanseniaspora and Starmerella) in the Cellar

2016

International audience; Different genera and/or species of yeasts present on grape berries, in musts and wines are widely described. Nevertheless, the community of non-Saccharomyces yeasts present in the cellar is still given little attention. Thus it is not known if the cellar is a real ecological niche for these yeasts or if it is merely a transient habitat for populations brought in by grape berries during the winemaking period. This study focused on three species of non-Saccharomyces yeasts commonly encountered during vinification: Starmerella bacillaris (synonymy with Candida zemplinina), Hanseniaspora guilliermondii and Hanseniaspora uvarum. More than 1200 isolates were identified at …

0301 basic medicineMicrobiology (medical)Grape berriesCerevisiaeWine yeast[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition030106 microbiologyStarmerellalcsh:QR1-502StrainsCandida-stellataHanseniasporaMicrobiologySaccharomyceslcsh:MicrobiologyHanseniaspora03 medical and health sciences[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologycellarBotanyOriginal ResearchWinemakingDiversitybiologyfungifood and beveragespersistenceEnological propertiesbiology.organism_classificationSulfur-dioxideYeastCandida zemplininaYeast in winemaking[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitologynon-Saccharomyces strainsStarmerellaHanseniaspora guilliermondiiAlcoholic fermentationPopulation-dynamicsFrontiers in Microbiology
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Time Series Analysis of the Microbiota of Children Suffering From Acute Infectious Diarrhea and Their Recovery After Treatment

2018

WOS: 000434952800001

0301 basic medicineMicrobiology (medical)Rotavirusmedicine.medical_specialtytemporal analysis030106 microbiologylcsh:QR1-502acute infectious diarrheaDiseaseGut floramedicine.disease_causeMicrobiologylcsh:Microbiologylaw.invention03 medical and health sciencesProbioticAcute infectious diarrhealawInternal medicineRotavirusmedicinemicrobiotaFecesOriginal Researchbiologybusiness.industryMicrobiotasystems biologyTemporal analysisbiology.organism_classificationmedicine.diseaseDiarrhea030104 developmental biologyrotavirusmedicine.symptombusinessSystems biologyDysbiosisSaccharomyces boulardiiFrontiers in Microbiology
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