Search results for "SAC"
showing 10 items of 3337 documents
Expression of a plant serine O-acetyltransferase inSaccharomyces cerevisiae confers osmotic tolerance and creates an alternative pathway for cysteine…
2004
Screening of a sugar beet (Beta vulgaris cv. Dita) cDNA library for clones able to confer osmotic tolerance to the osmosensitive gpd1 mutant of Saccharomyces cerevisiae identified a novel serine O-acetyltransferase (BvSAT; EC 2.3.1.30). This enzyme is involved in cysteine biosynthesis in plants and bacteria, producing O-acetylserine, which is converted into cysteine in a reaction catalysed by O-acetylserine sulphydrylase (EC 4.2.99.8). This pathway is not conserved in yeast, where cysteine is synthesized in a four-step pathway starting with homoserine and having O-acetylhomoserine, homocysteine and cystathionine as intermediates. Expression of BvSAT in yeast takes advantage of the activity …
Identification of a novel Drosophila melanogaster gene, angel, a member of a nested gene cluster at locus 59F4,5.
1996
The identification of a novel Drosophila melanogaster gene, angel, is presented in this study. angel is located on the right arm of the second chromosome at locus 59F5, close to the nested genes l(2)tid, l(2)not, l(2)rot and l(2)dtl. We describe the genetic and molecular localization of angel and present its temporal expression in the wild-type. The deduced amino acid sequence of the ANG39 protein is characterized by a nuclear localization signal. Furthermore, the central part of the predicted ANG39 protein shows significant homology to the C-terminal portion of the yeast transcriptional effector CCR4.
Cloning and expression of a cDNA copy of the viral K28 killer toxin gene in yeast
1995
The killer toxin K28, secreted by certain killer strains of the yeast Saccharomyces cerevisiae is genetically encoded by a 1.9 kb double-stranded RNA, M-dsRNA (M28), that is present within the cell as a cytoplasmically inherited virus-like particle (VLP). For stable maintenance and replication, M28-VLPs depend on a second dsRNA virus (LA), which has been shown to encode the major capsid protein (cap) and a capsid-polymerase fusion protein (cap-pol) that provides the toxin-coding M-satellites with their transcription and replicase functions. K28 toxin-coding M28-VLPs were isolated, purified and used in vitro for the synthesis of the single-stranded M28 transcript, which was shown to be of pl…
Tumor suppression inDrosophila is causally related to the function of thelethal(2)tumorous imaginal discs gene, adnaJ homolog
1995
The Drosophila melanogaster tumor suppressor gene lethal(2)tumorous imaginal discs (l(2)tid) causes in homozygotes malignant growth of cells of the imaginal discs and the death of the mutant larvae at the time of puparium formation. We describe the molecular cloning of the l(2)tid+ gene and its temporal expression pattern in the wild-type and mutant alleles. Germ line rescue of the tumor phenotype was achieved with a 7.0 kb Hindlll-fragment derived from the polytene chromosome band 59F5. The l(2)tid+ gene spans approximately 2.5 kb of genomic DNA. The protein coding region, 1,696 bps long, is divided by an intron into two exons. The predicted Tid56 protein contains 518 amino acids and posse…
Molecular cloning and characterization of aCandida albicansgene (EFB1) coding for the elongation factor EF-1β
1996
A Candida albicans gene homologous to Saccharomyces cerevisiae elongation factor 1 beta was isolated by screening a genomic DNA library using a C. albicans cDNA as a probe. This cDNA was previously obtained by immunoscreening of an expression library with polyclonal antibodies raised against candidal cell wall components. Sequence analysis of the cDNA and the whole C. albicans gene (EMBL accession number X96517) revealed an intron-interrupted open reading frame of 639 base pairs that encodes a 213 amino acid protein. Exon sequences are highly homologous (74%) to S. cerevisiae EFB1, whereas intron sequence is less conserved (34% identity), and the predicted amino acid sequence shares about 7…
Inventando el crimen pasional: del lenguaje de gacetilla a la prensa de sucesos (España, 1892-1920)
2021
El terme «crim passional» va sorgir a la premsa espanyola el 1892. L’objectiu d’aquest treball és desvelar el perquè i entendre’n la relació amb la premsa sensacionalista i, en especial, amb la premsa especialitzada en successos. La nostra metodologia considera que la premsa és un motor cultural en la construcció de conceptes amb incidència social. Així, doncs, explorem la presència del terme «crim passional» en un bon nombre de diaris i setmanaris per observar com es va construir un marc mental de naturalesa emocional. Es revela la incidència del concepte en l’opinió pública i en el llenguatge jurídic, així com la seva rellevància en la justificació de la violència contra les dones.
El Síndico de la ciudad de Valencia. De las Germanías a la insaculación
2001
El síndico de la ciudad de Valencia era uno de los principales cargos en el organigrama político municipal de la capital del Reino durante el periodo foral; de ahí que el sistema de provisión del mismo llegara a cobrar suficiente entidad como para suscitar deseos de control por parte de la ciudad y de la Corona. Además de analizar las características del oficio y la relación de quienes lo desempeñaron, el presente trabajo se centra en las disputas que ambos sectores de la administración mantuvieron sobre su designación, especialmente a partir del momento, a finales del Quinientos, en que se desdobla el cargo y deja de ser perpetuo, escapando al control tradicional que había venido mantenien…
Polysaccharides from Pleurotus eryngii var. elaeoselini (Agaricomycetes), a New Potential Culinary-Medicinal Oyster Mushroom from Italy.
2020
Three water-soluble glucans (PELPS-A1, PELPS-A2, and PELPS-A3) purified from the hot water extract of the basidiomata of an edible mushroom Pleurotus eryngii var. elaeoselini by chromatography on DEAE-cellulose 32 and Sephadex G-100 column were found to consist of only D-glucose as monosaccharide constituent. Structural investigation was carried out by acid hydrolysis, periodate oxidation, and NMR experiments (1H-NMR, 13C-NMR, DQF-COSY, TOCSY, ROESY, HMQC, and HMBC). On the basis of these experiments, the structures of the repeating unit of the three isolated polysaccharides were established as follows: (1) PELPS-A1: {[→3)-α-D-Glcp-(1→]3→4)-α-D-Glcp-(1→2)-α-D-Glcp-(1→6)-α-D-Glcp-(1[→6)-β-D-…
Daiļliteratūra kā līdzeklis vecākā pirmsskolas vecuma bērna radošās attīstības sekmēšanai pedagoģiskajā procesā
2017
Bakalaura darba “Daiļliteratūra kā līdzeklis vecākā pirmsskolas vecuma bērna radošās attīstības sekmēšanai pedagoģiskajā procesā” autore ir Rota Greiškalne. Mērķis ir pētīt vecākā pirmsskolas vecuma bērnu radošuma attīstības iespējas literāro darbu iepazīšanas procesā. Uzdevumi: Analizēt zinātniskās atziņas par radošumu un tā attīstību; Raksturot vecākā pirmsskolas vecuma raksturīgās attīstības iezīmes un radošuma attīstību to kontekstā. Empīriski pārbaudīt literāru darbu izmantošanas iespējas radošuma attīstības sekmēšanai pedagoģiskajā procesā. Izstrādāt secinājumus par radošuma attīstības sekmēšanas iespējām, iepazīstoties ar daiļliteratūru pirmsskolas pedagoģiskajā procesā. Hipotēze: Ve…
SAP tehnoloģijas pielietojums datu analītikai
2020
Pēdējos gados SAP tehnoloģijas attīstās arvien straujāk un tiek plaši lietotas datu analītikas jomā. Bakalaura darbā tiek apskatītas SAP kompānijas piedāvātie risinājumi datu analītikai, jo SAP tehnoloģijas mūsdienās skaitās viena no pašiem galvenajiem datu analītikas platformām un ir konkurētspējīgas tirgū. Darba mērķis ir aplūkot un veikt SAP kompānijas datu analītikai piedāvāto tehnoloģiju analīzi.