Search results for "SAMPLE"

Quantification of DNA and RNA: a Quantitative Visual Comparison

2002

The analytical procedure for the quantification of DNA and RNA described is the quantitative visual comparison of an unknown sample with known standards. This is quick and easy to do, suitable for amounts of sample as small as 10 ng. Keywords: DNA; RNA; rapid quantification

Comparative study of two sample treatments for a liquid chromatography–tandem mass spectrometry determination of mycotoxin biomarkers in urine

2016

Comparative assessment of solid-phase extraction clean-up procedures, GC columns and perfluoroacylation reagents for determination of type B trichoth…

2005

Abstract Various solid-phase extraction (SPE) procedures for clean-up, two perfluoroacylation reagents (pentafluoropropionic anhydride (PFPA) and heptafluorobutyric anhydride (HFBA)) and two chromatographic columns (HP-1701 and HP-5) have been assessed comparatively to achieve the determination of type B trichothecenes (deoxynivalenol (DON), nivalenol (NIV), 3- and 15-acetyldeoxynivalenol (3- and 15-ADON)) in wheat grain by gas chromatography (GC)–electron-capture detection (ECD). Spiked wheat samples were extracted with acetonitrile–water (84:16, v/v). Tested SPE procedures were MycoSep 225 column, Florisil and different cartridges prepared in the laboratory with mixtures of various sorben…

<strong>QUANTITATIVE QUANTUM MECHANICAL NMR ANALYSIS: THE SUPERIOR TOOL FOR ANALYSIS OF BIOFLUIDS</strong>

2016

Almost automatic quantitative analysis of biofluids is now behind only a few clicks from sample to EXCEL table after minimal sample preparation (move 0.3 ml sample into NMR tube and add buffer), without separations, calibration and reference materials, even for unknown compounds!  Each organic compound with protons gives a highly diagnostic and unique NMR spectrum which is practically identical with any spectrometer operating at certain field. A distinctive feature of high-resolution 1D NMR spectra is that even the most complex spectrum of a compound can be described by a few spectral parameters within experimental accuracy, employing a quantum mechanical theory. The NMR spectral parameters…

Random quasi-phase-matched second harmonic generation in periodically poled lithium tantalate

2009

We experimentally observed and explained bulk second harmonic generation via random quasi-phase-matching, derived from a periodically poled lithium tantalate sample with a randomly patterned mark-to-space-ratio.

New developments in immunochemical water analysis down to 30 mul sample volume.

1999

Abstract The determination of trace levels of pesticides like atrazine in water samples of small, restricted volumes is one of the future demands of environmental analysis. In a brief review existing chromatographic and immunochemical methods for atrazine are critically discussed. Then a simple rapid enzyme-linked immunosorbent assay (ELISA) using the tip of an inoculation needle as a solid surface is presented. The sample volume could be reduced to 30 μl. The assay had a centre of the test IC50 of 0.12 μg l−1 and permitted the characterisation of atrazine at levels of 0.022–2.90 μg l−1. A first outlook for automatisation is given. The new method was compared with an ELISA using 96 well mic…

PSYCHIATRY * P61 * DIMENSIONS AND CATEGORIES OF DSM V CRITERIA IN AN INTERNATIONAL SAMPLE OF DRINKING SUBJECTS AND INPATIENT ALCOHOL-DEPENDENT INDIVI…

2011

Application of pressurized fluid extraction to determine cadmium and zinc in plants

2006

Abstract A procedure for the determination of Cd and Zn in plants is proposed. The metals are extracted by pressurized fluid extraction (PFE). Operational conditions are: pressure 1500 psi, temperature 75 °C, static time 5 min, flush volume 35%, purge time 60 s, cycles 1 and 1,2-diaminocyclohexane- N , N , N ′, N ′-tetraacetic acid (CDTA) 0.01 M at pH 4.5 as extracting solution. Determination of Zn is carried out by flame atomic absorption spectroscopy and depending on the concentration level, Cd content is determined by flame or electrothermal atomic absorption spectroscopy. Certified samples of Virginia tobacco leaves, tea leaves, spinach leaves, poplar leaves, a commercial spinach sample…

The impact of adverse childhood experiences. Evaluation in clinical and non clinical samples using the CECA Interview (Childhood Experience of Care a…

2011

Psychometric properties of the Cyber-Violence Scale in Adolescent Couples (Cib-VPA)

2018

Resumen La utilización que los adolescentes hacen de las redes sociales, móviles e internet, para ejercer violencia hacia la pareja resulta preocupante. Por esta razón, es necesario disponer de instrumentos fiables, específicos para población adolescente y que permitan evaluar tanto situaciones de acoso como de victimización. El objetivo de este estudio fue analizar las propiedades psicométricas de la Escala de Ciber-violencia en Parejas Adolescentes (Cib-VPA). Esta escala consta de dos subescalas, ciberacoso perpetrado y cibervictimización, con 10 ítems cada una relativos a conductas agresivas y de control que pueden ser perpetradas o sufridas a través de redes sociales en parejas adolesce…