Search results for "SAR"

STAT1 and Its Crucial Role in the Control of Viral Infections

2022

The signal transducer and activator of transcription (STAT) 1 protein plays a key role in the immune response against viruses and other pathogens by transducing, in the nucleus, the signal from type I, type II and type III IFNs. STAT1 activates the transcription of hundreds of genes, some of which have been well characterized for their antiviral properties. STAT1 gene deletion in mice and complete STAT1 deficiency in humans both cause rapid death from severe infections. STAT1 plays a key role in the immunoglobulin class-switch recombination through the upregulation of T-bet; it also plays a key role in the production of T-bet+ memory B cells that contribute to tissue-resident humoral memory…

SARS-CoV-2 envelope protein topology in eukaryotic membranes

2020

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…

Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme

1997

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…

Tiešsaistes apdrošināšanas platformas optimizācija

2018

Vispārīgā datu aizsardzības regula (VDAR) ir paredzēta datu aizsardzībai un privātuma nodrošināšanai visiem pilsoņiem, kas dzīvo Eiropas Savienības valstīs. Viena no šīs regulas prasībām ir datu subjektu pieprasījums (DSP), kas paredz kādas organizācijas pakalpojumu lietotājam pieprasīt par sevi vāktos datus, kas glabājas organizācijā. Arī tiešsaistes apdrošināšanas platformai Bamboo, kas tiek izstrādāta uzņēmumā, ir jānodrošina tās klientiem iegūt par sevi uzglabātos datus. Pašlaik tas tiek nodrošināts manuāli, ejot cauri visiem dokumentiem un lejupielādējot tos pa vienam. Tādēļ šī darba mērķis ir optimizēt platformu un atvieglot darbu zvanu centra darbiniekiem, kas pārvalda klientu datus …

Hidalgo ja käsikirjoittaja : tekijän näkökulma adaptaatioon ja visuaaliseen kerrontaan laajassa sarjakuvassa

2006

Volte tabicadas nelle grandi isole del Mediterraneo: Sicilia e Sardegna (XV-XVIII secolo)

2012

Allo stato attuale delle ricerche le volte"tabicadas" costituiscono per la Sicilia e la Sardegna una scoperta recente, troppo episodici risultano oggi i casi conosciuti. Paradossalmente sono le fabbriche più precoci, tutte in Sicilia (torre di Pozzallo, castello di Butera, convento di Santa Maria di Gesù a Modica), quelle che presentano minori difficoltà storiografiche, poichè direttamente collegabili a committenti catalani e quindi a esperienze costruttive di importazione. Più problematici appaiono gli esempi in importanti edifici civili della Palermo del Settecento e individuabili anche attraverso le fonti notarili. Altrettando si può dire per cosiddetti "ladrillos de boveda"documentati i…

Maltrato y enfermedad mental. Estudio de personas con enfermedad mental, víctimas de maltrato

2011

Se realiza un trabajo de investigación sobre la victimización de personas con enfermedad mental, desde la perspectiva de que este colectivo es con frecuencia víctima de maltrato, a pesar de los prejuicios y el estigma que pende sobre él. Dada su especial vulnerabilidad social, los y las profesionales que atienden a esta población tienen la responsabilidad de visibilizar este fenómeno, y validar los instrumentos de diagnóstico que utilizan como útiles para detectar maltrato. The thesis involved research on the victimization of people with mental illness from the angle of this group often being the victim of abuse, despite the prejudice and stigma that hangs over it. Given its special social …

Developing response-ability in human-wasp encounters

2023

Learning to live with unloved others is crucial in the ecological crisis. Unloved wasps are feared and disliked for their sting. Understanding of their ecological importance is increasing, however. Human-wasp encounters are changing with environmental changes, and strategies for multispecies cohabitation are needed. This multispecies study highlights features of wasp biology affecting human-wasp encounters and analyzes how conflicted human-wasp relations could be mitigated. The geographical focus is Finland, in the Northern boreal region. Biological analysis specified spatiotemporal aspects affecting human-wasp encounters: human and wasp habitats and preferences overlap ubiquitously. From a…

Anàlisi sobre la figura/institució del Comissionat del menor: Pare d'Òrfens contemplada a la Llei 12/2008 de Protecció a la infància i l'adolescència…

2012

The concept / institution of the Commissioner for Children ' 'Pare d'Òrfens' appears for the first time in Valencia with Law 12/2008 on Protection of Children and Adolescents. The purpose of this article is to inform readers of the existence of this concept / institution, analysing the regulatory development that has taken place and comparing its performance with other similar concepts/institutions existing in other Spanish regions, in order to critically assess the work done in recent years. To close the paper, we give a number of conclusions drawn after completing the research on this concept / institution, including several general recommendations in the form of constructive criticism.

What makes John move? Outdoor play physical environmental factors changing a child’s activity from sedentary to physically active: longitudinal mixed…

2021

Children’s low physical activity levels call for an investigation of what can promote activity for excessively sedentary children. The purpose of this longitudinal mixed-method case study in an early childhood education (ECE) environment was to observe one sedentary child’s free outdoor playtime in four seasons and determine the physical environmental factors changing his activity from sedentary to physically active. Systematic observation of video material and quantitative analysis were used to identify the most sedentary individual of the one ECE group. The child was named “John”. Qualitative analysis of John’s video material showed that during the four seasons he spent an average of 25% …