Search results for "SCOPE"
showing 10 items of 2420 documents
Interaction between biotin lipids and streptavidin in monolayers: formation of oriented two-dimensional protein domains induced by surface recognitio…
1989
Highly specific ligand-receptor interactions generally characterize surface recognition reactions. Such processes can be simulated by streptavidin-biotin-specific binding. Biotin lipids have thus been synthesized, and their interaction with streptavidin (or avidin) at the air-water interface was directly shown by measurement of surface pressure isotherms and fluorescence microscopy. These proteins interact with the biotin lipid monolayer via specific binding or nonspecific adsorption. Both phenomena were clearly distinguished by use of the inactivated form of streptavidin. The binding of fluorescein-labeled streptavidin to monolayers was also directly observed by fluorescence microscopy. Th…
Formation of protein multilayers and their competitive replacement based on self-assembled biotinylated phospholipids.
1994
Based on specific recognition processes the build-up of protein multilayers was achieved using streptavidin layers as a docking matrix. For this purpose, streptavidin was organized at biotin-containing monolayers, liposomes, and self-assembled layers on gold. Thus, mixed double and triple layers of streptavidin, Con A, Fab fragments, and hormones were prepared and characterized by fluorescence microscopy and plasmon spectroscopy. Using biotin analogues with lower binding constants several cycles of multilayer formation followed by competitive replacement could be achieved.
Surface Development of (As2S3)1–x (AgI)x Thin Films for Gas Sensor Applications
2011
Thin (As2S3)100−x(AgI)x (x = 0–40) films were deposited by thermal vacuum evaporation from the respective bulk glasses; their structure and morphology before and after illumination with light have been studied by scanning electron microscopy (SEM). As-deposited films show fractional evaporation and surface inhomogeneities but after illumination they become uniform on the surface and in the depth as revealed by SEM top-view and cross-section images. Mechanical parameters like stress and microhardness of as-prepared and illuminated films were also investigated. The results from the stress measurements show variation in both the sign and the magnitude of the values with increasing AgI content …
Discourse Markers in Speech: Distinctive Features and Corpus Annotation
2017
It is generally acknowledged that discourse markers are used differently in speech and writing, yet many general descriptions and most annotation frameworks are written-based, thus partially unfit to be applied in spoken corpora. This paper identifies the major distinctive features of discourse markers in spoken language, which can be associated with problems related to their scope and structure, their meaning and their tendency to co-occur. The description is based on authentic examples and is followed by methodological recommendations on how to deal with these phenomena in more exhaustive, speech-friendly annotation models.
What Would a Unified Field of Listening Look Like? A Proposal Linking Past Perspectives and Future Endeavors
2008
Most reviews of “listening research” are narrow in scope, focusing only on research published by listening and communication scholars. Given that unique contributions to listening have been provided by scholars from disciplines as varied as psychology, anthropology, management, and linguistics, this review explores connections and divergences that span the academic landscape. After briefly introducing and reviewing listening related research from three primary areas—information processing, competent behavior, and individual differences—we offer a heuristic framework that ties these lines of study together and provides a structure for assessing and generating new listening research. A conclu…
A Proposal to Model Ancient Silk Weaving Techniques and Extracting Information from Digital Imagery - Ongoing Results of the SILKNOW Project
2019
Three dimensional (3D) virtual representations of the internal structure of textiles are of interest for a variety of purposes related to fashion, industry, education or other areas. The modeling of ancient weaving techniques is relevant to understand and preserve our heritage, both tangible and intangible. However, ancient techniques cannot be reproduced with standard approaches, which usually are aligned with the characteristics of modern, mechanical looms. The aim of this paper is to propose a mathematical modelling of ancient weaving techniques by means of matrices in order to be easily mapped to a virtual 3D representation. The work focuses on ancient silk textiles, ranging from the 15…
Introduction to the Book
2016
This chapter sets the scene for the book. The three sections, respectively: state the purpose and scope of the book; present two attempts at answering the question ‘what is a tool?’; and outline the structure of the book.
Automated electron diffraction tomography – development and applications
2019
Electron diffraction tomography, a potential method for structure analysis of nanocrystals, and, in more detail, the strategies to use automated diffraction tomography (ADT) technique are described. Examples of ADT application are discussed according to the material class.
Effects of an innovative dipping treatment on the cold storage of minimally processed Annurca apples
2007
The effect of trehalose as an edible coating on minimally processed Annurca apple slices was studied during cold storage. The edible coating was prepared by dipping the fruit in a solution containing trehalose at 0.8%, sucrose at 1.0% and sodium chloride at 0.1%. During storage at 6 degrees C the following parameters were monitored: weight loss, colour (hue angle (h degrees) and whitening index (WI)), firmness, malic and ascorbic acids, polyphenol content.. microstructure by scanning electron microscopy (SEM) and microbial count. The results showed that such a coating reduced the browning phenomena; in fact the WI and h degrees values were significantly lower in coated samples than untreate…
Easy removal of a large mucus plug with a flexible paediatric bronchoscope after administration of rhDNase (Pulmozyme).
2006
Mucoid impaction and plastic bronchitis are relatively rare disorders affecting patients of any age. Here we report the case of mucoid impaction developing in a four-year-old child as a complication of pneumonic infection treated four weeks prior. p.a. chest X-ray and fiberoptic bronchoscopy showed atelectasis and the location of a mucus cast in the left upper lobe. After extraction of the cast via the suction channel of the bronchoscope had failed, we administered 2.5 mg of undiluted recombinant human deoxyribonuclease (rhDNase) instilled directly over the affected area of occlusion for 15 minutes. This mucolytic agent mobilized the cast by reducing viscoelasticity and adherence of mucus. …