Search results for "SECRETION"

showing 10 items of 764 documents

Sentinel hospital-based surveillance for norovirus infection in children with gastroenteritis between 2015 and 2016 in Italy

2018

Noroviruses are one of the leading causes of gastro-enteric diseases worldwide in all age groups. Novel epidemic noroviruses with GII.P16 polymerase and GII.2 or GII.4 capsid type have emerged worldwide in late 2015 and in 2016. We performed a molecular epidemiological study of the noroviruses circulating in Italy to investigate the emergence of new norovirus strains. Sentinel hospital-based surveillance, in three different Italian regions, revealed increased prevalence of norovirus infection in children (<15 years) in 2016 (14.4% versus 9.8% in 2015) and the emergence of GII.P16 strains in late 2016, which accounted for 23.0% of norovirus infections. The majority of the strains with a GII.…

0301 basic medicineRNA virusesEuropean PeopleSettore MED/07 - Microbiologia E Microbiologia Clinicavirusesmedicine.disease_causePathology and Laboratory MedicinePediatricsGeographical locationsfluids and secretionsEpidemiologyGenotypePrevalenceMedicine and Health SciencesEthnicitiesChildCaliciviridae InfectionsMultidisciplinaryIncidence (epidemiology)Database and informatics methodsQRSequence analysisvirus diseasesGastroenteritisItalian PeopleEuropeCapsidItalyMedical MicrobiologyChild PreschoolViral PathogensVirusesMedicineRNA ViralPathogensPediatric InfectionsResearch Articlemedicine.medical_specialtyGenotypingGenotypeBioinformaticsScience030106 microbiologySequence DatabasesMicrobiologyCaliciviruses03 medical and health sciencesAge groupsmedicineHumansEuropean UnionMolecular Biology TechniquesGenotypingMicrobial PathogensMolecular BiologyBiochemistry Genetics and Molecular Biology (all)RNA sequence analysisBiology and life sciencesbusiness.industrySequence Analysis RNANorovirusOrganismsGenetic VariationRNA-Dependent RNA PolymeraseVirologydigestive system diseasesResearch and analysis methods030104 developmental biologyCaliciviridae InfectionsBiological DatabasesAgricultural and Biological Sciences (all)NorovirusCapsid ProteinsPopulation GroupingsPeople and placesbusinessSentinel Surveillance
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Assessing the burden of viral co-infections in acute gastroenteritis in children: An eleven-year-long investigation.

2020

Abstract Background Acute gastroenteritis is an important cause of childhood morbidity and mortality worldwide. A number of pathogens are responsible for human acute gastroenteritis. The recent introduction of syndromic assays for the diagnosis of enteric infections, including a wide panel of enteric pathogens, has unveiled the frequency of mixed infections. This study was carried out to assess the burden of viral co-infections and the genetic diversity of the viruses detected in children hospitalized with acute gastroenteritis in Italy. Methods A total of 4161 stool samples collected from diarrheic children over 11 years, from January 2008 to December 2018, were investigated for the presen…

0301 basic medicineRotavirusSettore MED/07 - Microbiologia E Microbiologia Clinicamedicine.medical_specialtyviruses030106 microbiologymedicine.disease_causeVirusAstrovirus03 medical and health sciencesFecesfluids and secretions0302 clinical medicineVirologyRotavirusEpidemiologyGenotypeMedicineHumans030212 general & internal medicineChildFecesbiologybusiness.industryCoinfectionNorovirusvirus diseasesInfantbiology.organism_classificationVirologyGastroenteritisCo-infection Ct values Enteric viruses Genotypes Real-time PCR.Infectious DiseasesItalyNorovirusbusinessViral loadJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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Interactions between saliva and flavour compounds

2016

Interactions between saliva and flavour compounds

0301 basic medicineSaliva030109 nutrition & dieteticsChemistryHigh Energy Physics::Lattice[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionFlavourHigh Energy Physics::PhenomenologyPhysics::Medical Physics04 agricultural and veterinary sciences040401 food sciencesalivation03 medical and health sciencesstomatognathic diseases[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition0404 agricultural biotechnologyfluids and secretionsstomatognathic systemaromaHigh Energy Physics::ExperimentCondensed Matter::Strongly Correlated ElectronsFood science[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
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Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals

2019

Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes. Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed. We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects. Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored. We assessed salivary and feces microbiota compositio…

0301 basic medicineSalivamedicine.medical_treatmentHIV Infectionslcsh:TX341-641Gut floradigestive systemArticleBacterial geneticsFeces03 medical and health sciences0302 clinical medicinefluids and secretionsmedicinemicrobiotaHumansMicrobiomeprebiotic interventionbacteria co-occurrenceFecessalivaNutrition and DieteticsbiologyPrebioticHIV030206 dentistryRibosomal RNAbiology.organism_classificationmedicine.diseaseGastrointestinal MicrobiomeRNA Bacterialstomatognathic diseasesPrebiotics030104 developmental biologyImmunologygutDysbiosislcsh:Nutrition. Foods and food supplyFood Science
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The effects of graded caloric restriction: XII. Comparison of mouse to human impact on cellular senescence in the colon.

2018

Calorie restriction (CR) is an effective strategy to delay the onset and progression of aging phenotypes in a variety of organisms. Several molecular players are involved in the anti-aging effects of CR, but mechanisms of regulation are poorly understood. Cellular senescence—a cellular state of irreversible growth arrest—is considered a basic mechanism of aging. Senescent cells accumulate with age and promote a number of age-related pathologies. Whether environmental conditions such as diet affect the accumulation of cellular senescence with age is still unclear. Here, we show that a number of classical transcriptomic markers of senescent cells are reduced in adult but relatively young mice…

0301 basic medicineSenescenceAgingColonCalorie restrictionAdipose tissueBiologySASPTranscriptome03 medical and health sciencesMiceAnimalsHumanscellular senescenceSecretionMechanism (biology)Short TakeCell Biologyageing aging caloric restriction cellular senescence SASPPhenotypeCell biologyDietDisease Models Animal030104 developmental biologyADIPOSE-TISSUEAgeingageingCELLSSECRETIONcaloric restrictionAging cell
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Helicobacter pylori and Epstein–Barr Virus Infection in Gastric Diseases: Correlation with IL-10 and IL1RN Polymorphism

2019

Introduction. Helicobacter pylori and Epstein–Barr virus (EBV) infection have recently been shown to be associated with gastric diseases. Polymorphisms in genes encoding cytokines such as interleukin 10 (IL-10) and interleukin 1 Receptor (IL-1RN) influence cytokine secretion levels and appear to contribute to the risk of developing gastroduodenal diseases. To our knowledge, this is the first preliminary study to address the association of coinfection with H. pylori and EBV and their correlation with genetic predisposition in the development of gastric diseases. Methods. Gastric biopsy samples of 96 patients with different gastric diseases were used. Results. Our results showed that the rate…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaArticle Subjectpolymorphism gastric cancer IL-10Chronic gastritislcsh:RC254-28203 medical and health sciences0302 clinical medicineEBVHelicobactermedicineCagAEpstein–Barr virus infectionbiologybusiness.industryMALT lymphomaHelicobacter pylorimedicine.diseasebiology.organism_classificationlcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensdigestive system diseases030104 developmental biologyOncology030220 oncology & carcinogenesisImmunologyCoinfectionCytokine secretionGastritismedicine.symptombusinessResearch ArticleJournal of Oncology
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Norovirus GII.17 as Major Epidemic Strain in Italy, Winter 2015–16

2017

In winter 2015-16, norovirus GII.17 Kawasaki 2014 emerged as a cause of sporadic gastroenteritis in children in Italy. Median patient age was higher for those with GII.17 than GII.4 infection (55 vs. 24 months), suggesting limited cross-protection for older children.

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaEpidemiologyviruseslcsh:Medicinemedicine.disease_causeDisease Outbreaksfluids and secretionsEpidemiologyChildEpidemic strainCaliciviridae InfectionsNorovirus GIIvirus diseasesInfectious DiseasesItalyChild PreschoolPopulation SurveillanceSeasonsgastroenteritigastroenteritisNorovirus GII.17 as Major Epidemic Strain in Italy Winter 2015–16Microbiology (medical)medicine.medical_specialtyAdolescentGenotype030106 microbiologyenteric infectionHistory 21st Centurylcsh:Infectious and parasitic diseasesOpen Reading Frames03 medical and health sciencesPatient ageResearch LettermedicineHumansviruseslcsh:RC109-216Noroviruvirusebusiness.industryenteric infectionsNoroviruslcsh:RInfant NewbornInfantGII.17 Kawasaki 2014Virology030104 developmental biologyNorovirusbusinessEmerging Infectious Diseases
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Characterization of a mutant form of human apolipoprotein B (Thr26_Tyr27del) associated with familial hypobetalipoproteinemia

2016

We have previously identified a deletion mutant of human apoB [apoB (Thr26_Tyr27del)] in a subject with primary hypobetalipoproteinemia. The present study determined the effect of Thr26_Tyr27del mutation on apoB secretion using transfected McA-RH7777 cells. Transient or stable transfection of apoB-48 containing the Thr26_Tyr27del mutation showed drastically reduced secretion of the mutant as compared to wild-type apoB-48. No lipoproteins containing the mutant apoB-48 were secreted into the medium. Incubation of transfected cells in a lipid-rich medium in the presence of cycloheximide showed rapid turnover of cell-associated mutant apoB-48 as compared to that of wild-type apoB-48. Immunofluo…

0301 basic medicineSettore MED/09 - Medicina InternaTime FactorsApolipoprotein B-48 secretionApolipoprotein BMutantDNA Mutational AnalysisApolipoprotein B mutation Apolipoprotein B-48 secretion Hypobetalipoproteinemia Proteasomal degradation030204 cardiovascular system & hematologymedicine.disease_causeEndoplasmic ReticulumHypobetalipoproteinemiaschemistry.chemical_compound0302 clinical medicineProteasomal degradationProteolysiSequence DeletionMutationbiologyMedicine (all)TransfectionProteasome InhibitorPhenotypeBiochemistryApolipoprotein B-100lipids (amino acids peptides and proteins)Proteasome InhibitorsHumanHeterozygoteProteasome Endopeptidase ComplexTime FactorCycloheximideTransfectiondigestive systemCell LineDNA Mutational Analysi03 medical and health sciencesmedicineHumansSecretionGenetic Predisposition to DiseaseMolecular BiologyEndoplasmic reticulumnutritional and metabolic diseasesCell Biologymedicine.diseaseMolecular biology030104 developmental biologychemistryProteolysisbiology.proteinHypobetalipoproteinemiaApolipoprotein B mutationApolipoprotein B-48Hypobetalipoproteinemia
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Tubercle disease of sugar beet roots (Beta vulgaris) found in Poland is neither caused by Xanthomonas beticola nor by tumorigenic Agrobacterium/Rhizo…

2018

Symptoms of tubercle disease known also as Xanthomonas gall were noticed in Poland in 2014–2017. Roots with disease symptoms showed the reduction of sucrose content compared to the healthy ones. In the literature, there are two gall diseases described: tubercle disease (also known as Xanthomonas gall) and crown gall, which is caused by Rhizobium radiobacter (syn. Agrobacterium tumefaciens). None of the bacterial strains isolated from the malformed tissues were identified as the bacterial pathogen responsible for causing Xanthomonas gall or crown gall. The result was confirmed by searching for the presence of T-DNA, which was not found. Our conclusion is that the tubercle disease observed in…

0301 basic medicineTubercleAgrobacteriumPlant ScienceHorticultureRhizobium radiobacterdigestive systemMicrobiology03 medical and health sciencesfluids and secretionsTubercle diseaseXanthomonasGallPathogenbiologySugar beetdigestive oral and skin physiologyXanthomonas gallAgrobacterium tumefaciensbiology.organism_classificationdigestive system diseases030104 developmental biologyAgrobacterium tumefaciensRhizobiumSugar beetAgronomy and Crop ScienceJournal of Plant Diseases and Protection
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Selective targeting of collagen IV in the cancer cell microenvironment reduces tumor burden

2018

Goodpasture antigen-binding protein (GPBP) is an exportable1 Ser/Thr kinase that induces collagen IV expansion and has been associated with chemoresistance following epithelial-to-mesenchymal transition (EMT). Here we demonstrate that cancer EMT phenotypes secrete GPBP (mesenchymal GPBP) which displays a predominant multimeric oligomerization and directs the formation of previously unrecognized mesh collagen IV networks (mesenchymal collagen IV). Yeast two-hybrid (YTH) system was used to identify a 260SHCIE264 motif critical for multimeric GPBP assembly which then facilitated design of a series of potential peptidomimetics. The compound 3-[4''-methoxy-3,2'-dimethyl-(1,1';4',1'')terphenyl-2'…

0301 basic medicineTumor microenvironmentChemistryKinaseMesenchymal stem cellEMTPhenotype03 medical and health sciences030104 developmental biologyOncologyGPBPPrecursor cellCancer cellCancer researchmedicinecollagen IVtumor microenvironmentDoxorubicinSecretiondrug-resistant cancermedicine.drugResearch PaperOncotarget
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