Search results for "SERUM"

showing 10 items of 786 documents

Antiendomysium antibodies assay in the culture medium of intestinal mucosa: an accurate method for celiac disease diagnosis

2011

Background Celiac disease (CD) diagnosis is becoming more difficult as patients with no intestinal histology lesions may also be suffering from CD. Aim To evaluate the diagnostic accuracy of antiendomysium (EmA) assay in the culture medium of intestinal biopsies for CD diagnosis. Patients and methods The clinical charts of 418 patients with CD and 705 non-CD controls who had all undergone EmA assay in the culture medium were reviewed. Results EmA assay in the culture medium had a higher sensitivity (98 vs. 80%) and specificity (99 vs. 95%) than serum EmA/antibodies to tissue transglutaminase (anti-tTG) assay. All patients with CD who were tested as false-negatives for serum EmA and/or anti-…

AdultMalemedicine.medical_specialtyPathologySettore MED/09 - Medicina InternaAdolescentTissue transglutaminaseDuodenumBiopsyMuscle Fibers Skeletalceliac disease culture system diagnosis intestinal histology serum antiendomysiumDiseaseHuman leukocyte antigenGastroenterologyAntiendomysium antibodiesTissue Culture TechniquesYoung AdultIntestinal mucosaInternal medicineBiopsyMedicineHumansVillous atrophyIntestinal MucosaChildFalse Negative ReactionsAgedAutoantibodiesTransglutaminasesHepatologybiologymedicine.diagnostic_testbusiness.industryGastroenterologyInfantMiddle AgedCulture MediaCeliac DiseaseChild Preschoolbiology.proteinFemaleAntibodybusinessEpidemiologic MethodsBiomarkers
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Long-term sequential deferiprone-deferoxamine versus deferiprone alone for thalassemia major patients: a randomised clinical trial

2009

A multicentre randomized open-label trial was designed to assess the effectiveness of long-term sequential deferiprone–deferoxamine (DFO–DFP) versus DFP alone to treat thalassaemia major (TM). DFP at 75 mg/kg, divided into three oral daily doses, for 4 d/week and DFO by subcutaneous infusion (8–12 h) at 50 mg/kg per day for the remaining 3 d/week was compared with DFP alone at 75 mg/kg, administered 7 d/week during a 5-year follow-up. The main outcome measures were differences between multiple observations of serum ferritin concentrations. Secondary outcomes were survival analysis, adverse events, and costs. Consecutive thalassaemia patients (275) were assessed for eligibility; 213 of these…

AdultMalemedicine.medical_specialtyRandomizationAdolescentPyridonesAdministration OralKaplan-Meier EstimateDeferoxamineInfusions SubcutaneousIron Chelating AgentsGastroenterologylaw.inventionYoung Adultchemistry.chemical_compoundRandomized controlled triallawInternal medicinemedicineHumansDeferiproneAdverse effectDecreased serum ferritinSurvival analysisbusiness.industryHematologySurgeryClinical trialDeferoxamineChelation thalassaemia clinical trials red blood cell disorders iron overload.Treatment OutcomechemistryFerritinsThalassemiaDrug Therapy CombinationFemalebusinessDeferiproneFollow-Up Studiesmedicine.drug
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Analysis of thiamine transporter genes in sporadic beriberi

2014

Abstract Objective Thiamine or vitamin B 1 deficiency diminishes thiamine-dependent enzymatic activity, alters mitochondrial function, impairs oxidative metabolism, and causes selective neuronal death. We analyzed for the first time, the role of all known mutations within three specific thiamine carrier genes, SLC19 A2, SLC19 A3 , and SLC25 A19 , in a patient with atrophic beriberi, a multiorgan nutritional disease caused by thiamine deficiency. Methods A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema, a subacute sensorimotor neuropathy, and incontinence. Despite normal vitamin B 1 serum levels, his clinical picture was rapidly reverted by high-dose in…

AdultMalemedicine.medical_specialtySLC19 A- SLC25 A19SLC19 AEndocrinology Diabetes and MetabolismGene mutationBeriberimedicine.disease_causeMitochondrial Membrane Transport Proteinslaw.inventionBeriberilawInternal medicineGenotypemedicineThiamine transporterObjective: Thiamine or vitamin B1 deficiency diminishes thiamine-dependent enzymatic activity alters mitochondrial function impairs oxidative metabolism and causes selective neuronal death. We analyzed for the first time the role of all known mutations within three specific thiamine carrier genes SLC19 A2 SLC19 A3 and SLC25 A19 in a patient with atrophic beriberi a multiorgan nutritional disease caused by thiamine deficiency. Methods: A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema a subacute sensorimotor neuropathy and incontinence. Despite normal vitamin B1 serum levels his clinical picture was rapidly reverted by high-dose intramuscular thiamine treatment suggesting a possible genetic resistance. We used polymerase chain reaction followed by amplicon sequencing to study all the known thiamine-related gene mutations identified within the Human Gene Mutation Database. Results: Thirty-seven mutations were tested: 29 in SLC19 A2 6 in SLC19 A3 and 2 in SLC25 A19. Mutational analyses showed a wild-type genotype for all sequences investigated. Conclusion: This is the first genetic study in beriberi disease. We did not detect any known mutation in any of the three genes in a sporadic dry beriberi patient. We cannot exclude a role for other known or unknown mutations in the same genes or in other thiamine-associated genes in the occurrence of this nutritional neuropathy.HumansThiamineGenePolymerase chain reactionGeneticsMutationNutrition and DieteticsbiologyMembrane Transport ProteinsThiamine Deficiencymedicine.diseaseAlcoholismEndocrinologyMutationbiology.proteinThiamineMutations
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Ultra-long-distance running and the liver.

1990

During an ultra-long-distance race (1000 km in 20 days) the influence of running was examined on the enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), gamma-glutamyl-transferase (GGT), and glutamate dehydrogenase (GLDH) with regard to their release from the liver cells or their induction. Furthermore the liver synthetic capacity was assayed by measuring the enzyme activity of cholinesterase and the concentration of serum albumin during the race. Of the 110 participants, 55 finished the race and only the results of these runners were used in our study. AP increased continuously from day 0 (mean = 102 U/L) to day 19 (mean = 120 U/L). A fivefo…

AdultMalemedicine.medical_specialtySerum albuminPhysical Therapy Sports Therapy and Rehabilitationdigestive systemRunningInternal medicinemedicineHumansOrthopedics and Sports MedicineSerum AlbuminCholinesteraseAgedchemistry.chemical_classificationAnalysis of VariancebiologyLiver cellGlutamate dehydrogenaseAlbuminMiddle Ageddigestive system diseasesEnzyme assayOxygenEnzymeEndocrinologychemistryLiverEnzyme Inductionbiology.proteinPhysical EnduranceAlkaline phosphataseFemaleLiver CirculationInternational journal of sports medicine
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Relationships between mild hyperuricaemia and aortic stiffness in untreated hypertensive patients.

2013

Background and aims: Clinical studies exploring the relationship between serum uric acid (SUA) and arterial stiffness yielded conflicting results. Only in a few of these studies, arterial distensibility was examined by measuring aortic pulse wave velocity (PWV), which is considered the gold standard for evaluating arterial stiffness. In none of the previous investigations was the influence of SUA on aortic distensibility assessed, taking into account the effect of albuminuria. The purpose of our study was to comprehensively analyse the relationships between SUA and aortic PWV in a group of essential hypertensive patients. Methods and results: We enrolled 222 untreated and uncomplicated hype…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaEndocrinology Diabetes and MetabolismMedicine (miscellaneous)HyperuricemiaArteriosclerosiPulse Wave AnalysisEssential hypertensionEssential hypertensionBody Mass IndexSerum uric acidVascular StiffnessRisk FactorsStatistical significanceInternal medicinemedicineAlbuminuriaHumansPulse wave velocityAortaTriglyceridesSettore MED/14 - NefrologiaMetabolic SyndromeUnivariate analysisNutrition and Dieteticsbusiness.industryArteriosclerosisMiddle Agedmedicine.diseaseSettore MED/11 - Malattie Dell'Apparato CardiovascolareUric AcidPulse wave velocityEndocrinologyAortic stiffneCross-Sectional StudiesHypertensioncardiovascular systemArterial stiffnessAlbuminuriaCardiologyAortic stiffnessFemalemedicine.symptomCardiology and Cardiovascular MedicinebusinessNutrition, metabolism, and cardiovascular diseases : NMCD
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Training volume, androgen use and serum creatine kinase activity.

1989

Serum creatine kinase (CK) activities were investigated in elite male strength athletes (n = 20) during normal weight training and bodybuilding training (one training session per day), during high volume strength training (two sessions per day) and during strength training (one session per day) with the use of high dose synthetic androgens (five athletes in each subgroup). The findings demonstrated that the increase in serum CK was highest in the subgroup using androgens. These results suggest that strength training with the use of androgenic steroids leads to higher serum CK activities than normal strength training.

AdultMalemedicine.medical_specialtySynthetic androgensStrength trainingmedicine.drug_classeducationPhysical Therapy Sports Therapy and RehabilitationAnabolic AgentsInternal medicineMedicineHumansOrthopedics and Sports MedicineCreatine KinaseExercisePhysical Education and Trainingbiologybusiness.industryAthletesAndrogenic steroidsGeneral MedicineAndrogenbiology.organism_classificationEndocrinologyNormal weightbiology.proteinSerum creatine kinaseCreatine kinasebusinessResearch Article
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Is there a relationship between varicocele and testosterone levels?

2019

The goal of this work was to study the relationship between presence of varicocele and testosterone serum levels in adulthood.A comparative, cross-sectional study of 387 men who consulted for erectile dysfunction. Age, body mass index (BMI), diabetes (DM), and presence of varicocele were related to testosterone levels through uni- and multi-variate analysis.A total of 248 cases (70.8%) had no varicocele, 46 (13.1%) had grade I varicocele, 36 (10.3%) grade II, and 20 (5.7%) grade III. The mean total testosterone levels were 4.77 ng/mL in the non-varicocele group and 4.34 ng/mL in the varicocele group (We were not able to establish a relationship between the presence of varicocele and decreas…

AdultMalemedicine.medical_specialtyTestosterone - serumbusiness.industryVaricocele030232 urology & nephrology030209 endocrinology & metabolismTestosterone (patch)medicine.diseaseBody Mass Index03 medical and health sciences0302 clinical medicineEndocrinologyCross-Sectional StudiesErectile DysfunctionInternal medicineVaricocelemedicineHumansTestosteroneGeriatrics and GerontologybusinessThe aging male : the official journal of the International Society for the Study of the Aging Male
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The order effect of combined endurance and strength loadings on force and hormone responses: effects of prolonged training

2014

Purpose To examine acute responses and recovery of force and serum hormones to combined endurance and strength loadings utilizing different orders of exercises before and after training. Methods Physically active men were matched to an order sequence of endurance followed by strength (E + S, n = 12) or strength followed by endurance (S + E, n = 17). The subjects performed one experimental loading consisting of steady-state cycling and a leg press protocol before and after 24 weeks of order-specific combined training. Results No between-group difference in acute reductions of force was observed at week 0 (E + S −23 %, p < 0.001; S + E −22 %, p < 0.01) and 24 (E + S −25 %, p < 0.001; S + E −2…

AdultMalemedicine.medical_specialtyTime FactorsHydrocortisonePhysiologyOrder effectTraining adaptationsGrowth hormoneTestosterone bloodPhysical medicine and rehabilitationRecoveryPhysiology (medical)medicineHumansOrthopedics and Sports MedicineTestosteroneConcurrent trainingEndurance cyclingSerum hormonesFatigueCombined trainingbusiness.industryConcurrent trainingPublic Health Environmental and Occupational HealthResistance trainingResistance TrainingGeneral MedicineHuman physiologyCase-Control StudiesGrowth HormonePhysical therapyPhysical EndurancetestosteronibusinessHormone
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Acute Neuromuscular and Endocrine Responses and Recovery to Single-Session Combined Endurance and Strength Loadings

2013

The purpose of this study was to investigate acute neuromuscular and endocrine responses and recovery to a single session of combined endurance and strength loading using 2 loading orders. Forty-two men were demographically matched to perform a single session of combined endurance + strength (E + S) or strength + endurance (S + E) loading. The strength loading was conducted on a leg press and included sets of power, maximal strength, and hypertrophic loads with an overall duration of 30 minutes. The endurance loading was conducted on a bike ergometer and performed by continuous cycling over 30 minutes at 65% of subject's individual maximal watts. Both loading conditions led to significant a…

AdultMalemedicine.medical_specialtyTime FactorsMovementOrder effectThyrotropinPhysical Therapy Sports Therapy and RehabilitationInternal medicineMaximal strengthmedicineHumansEndocrine systemTestosteroneOrthopedics and Sports MedicineLactic AcidMuscle StrengthExercise physiologyMuscle Skeletalta315Leg pressCreatine KinaseExerciseSerum testosteroneChemistryExplosive forceResistance TrainingGeneral MedicineBicyclingCross-Sectional StudiesEndocrinologyLower ExtremityGrowth HormonePhysical EnduranceSingle sessionJournal of Strength and Conditioning Research
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Chronic graft-versus-host disease: long-term results from a randomized trial on graft-versus-host disease prophylaxis with or without anti-T-cell glo…

2011

Abstract Previous randomized graft-versus-host disease (GVHD)-prophylaxis trials have failed to demonstrate reduced incidence and severity of chronic GVHD (cGVHD). Here we reanalyzed and updated a randomized phase 3 trial comparing standard GVHD prophylaxis with or without pretransplantation ATG-Fresenius (ATG-F) in 201 adult patients receiving myeloablative conditioning before transplantation from unrelated donors. The cumulative incidence of extensive cGVHD after 3 years was 12.2% in the ATG-F group versus 45.0% in the control group (P &lt; .0001). The 3-year cumulative incidence of relapse and of nonrelapse mortality was 32.6% and 19.4% in the ATG-F group and 28.2% and 33.5% in the contr…

AdultMalemedicine.medical_specialtyTime FactorsTransplantation ConditioningAdolescentmedicine.medical_treatmentImmunologyMedizinGraft vs Host DiseaseHematopoietic stem cell transplantationBiochemistryGastroenterologyDisease-Free Survivallaw.inventionRandomized controlled triallawRecurrenceInternal medicinemedicineHumansTransplantation HomologousCumulative incidenceSurvival rateAntilymphocyte SerumImmunosuppression Therapybusiness.industryIncidence (epidemiology)Hazard ratioHematopoietic Stem Cell TransplantationCell BiologyHematologyMiddle Agedmedicine.diseaseSurgeryTransplantationSurvival RateGraft-versus-host diseaseMethotrexateHematologic NeoplasmsChronic DiseaseCyclosporineFemalebusinessImmunosuppressive AgentsBlood
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