Search results for "SIDA"
showing 10 items of 1584 documents
A comprehensive RNA-Seq-based gene expression atlas of the summer squash (Cucurbita pepo) provides insights into fruit morphology and ripening mechan…
2021
[EN] Background Summer squash (Cucurbita pepo: Cucurbitaceae) are a popular horticultural crop for which there is insufficient genomic and transcriptomic information. Gene expression atlases are crucial for the identification of genes expressed in different tissues at various plant developmental stages. Here, we present the first comprehensive gene expression atlas for a summer squash cultivar, including transcripts obtained from seeds, shoots, leaf stem, young and developed leaves, male and female flowers, fruits of seven developmental stages, as well as primary and lateral roots. Results In total, 27,868 genes and 2352 novel transcripts were annotated from these 16 tissues, with over 18,0…
La enseñanza del derecho político en Valencia durante la Restauración
2001
La edad ontemporánea supone un profundo cambio en la ciencia jurídica. En la Restauración borbónica comienza una ciencia jurídica nueva que se acerca al mundo européo con profesores como Colmeiro, Posada o Santamaría de Paredes. Hay conciencia de cambiar los métodos pedagógicos y de la importancia de la labor científica; un testimonio es el informe de Rafael de Oloriz y la discusión sobre la reforma de la enseñanza que aparece en las actas de la facultad de fines de siglo.
Humanismo, soberanía y religión. Rafael Rodríguez de Cepeda (1850-1918), un catedrático de Valencia conservador
2008
Las grandes cuestiones políticas y jurídicas sobre el individuo y el Estado se debatieron en España durante el siglo x1x, entre quienes deseaban una profunda transformación y quienes mantenían posiciones conservadoras. El pensamiento conservador, aunque con cierta puesta al día, siguió aferrado a la defensa del altar y el trono: la religión y el absolutismo... Unos más que otros, los conservadores añoraban viejos tiempos y se mostraban contrarios a las novedades liberales. El jurista Rafael Rodríguez de Cepeda, desde la filosofía del derecho, es buen ejemplo de esa pervivencia de viejas ideas, que divide España a fines de aquel siglo en dos bloques de pensamiento diversos, contrapuestos.
Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis.
2000
Monoterpenoid indole alkaloids are a vast and structurally complex group of plant secondary compounds. In contrast to other groups of plant products which produce many glycosides, indole alkaloids rarely occur as glucosides. Plants of Rauvolfia serpentina accumulate ajmaline as a major alkaloid, whereas cell suspension cultures of Rauvolfia mainly accumulate the glucoalkaloid raucaffricine at levels of 1.6 g/l. Cell cultures do contain a specific glucosidase. known as raucaffricine-O-beta-D-glucosidase (RG), which catalyzes the in vitro formation of vomilenine, a direct intermediate in ajmaline biosynthesis. Here, we describe the molecular cloning and functional expression of this enzyme in…
Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically invol…
2006
Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 buffer and 11% PEG 4000 as precipitant. Crystals belong to space group I222 and diffract to 2.30 A, with unit-cell parameters a = 102.8, b = 127.3, c = 215.8 A.
Understanding phenolic acids inhibition of α-amylase and α-glucosidase and influence of reaction conditions
2022
Phenolic acids are involved in modulating the activity of starch digestive enzymes but remains unclear if their interaction with enzymes or starch is governing the inhibition. The potential inhibition of nine phenolic acids against α-amylase and α-glucosidase was studied applying different methodologies to understand interactions between phenolic acids and either enzymes or substrates. Vanillic and syringic acids were prone to interact with α-amylase requiring low half-maximum inhibitory concentration (IC50) to inhibit starch hydrolysis. Nevertheless, the initial interaction of phenolic acids with starch somewhat obstructed their interaction with starch, requiring 10 times higher IC50, with…
Development of type-specific and cross-reactive serological probes for the minor capsid protein of human papillomavirus type 33.
1993
Human papillomavirus type 33 (HPV33) is associated with malignant tumors of the cervix. In an attempt to develop immunological probes for HPV33 infections, antisera against various bacterial fusion proteins carrying sequences of the minor capsid protein encoded by L2 were raised in animals. Antigenic determinants on the HPV33 L2 protein were identified by using truncated fusion proteins and were classified as type specific or cross-reactive with respect to HPV1, -8, -11, -16, and -18. Cross-reactive epitopes map to amino acids 98 to 107 or to amino acids 102 to 112 and 107 to 117, respectively, depending on the fusion protein used for immunization. Antibodies directed toward these epitopes …
Orchesting Inclusive Evaluation in Schools. Where We Could Start?
2019
Es cierto que en los últimos años se ha avanzado bastante en un discurso de la inclusividad potente y fundamentado, pero las prácticas siguen la inercia de una evaluación que parte de que todos los estudiantes son iguales. Se sigue arrastrando una cultura evaluativa asentada en que el aprendizaje está al servicio de la evaluación y la enseñanza termina subordinada a esta última. Una cultura reduccionista y calificadora muy arraigada, resistente al cambio y que cuesta mucho de reconstruir y transformar hacia una evaluación para la inclusión. En teoría hemos pasado de una evaluación centrada en la comprobación del saber, la certificación, el control, la calificación, etc. y en la que todos lo…
Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-…
1987
Micrococcal nuclease digestion has been used to investigate some fine details of the chromatin structure of the yeast SUC2 gene for invertase. Precisely positioned nucleosomes have been found on a 2 kb sequence from the 3' non-coding region, and four nucleosomes also seem to occupy fixed positions on the 5' flank. Eleven nucleosomes lie on the coding region, although their positioning is not as precise as in the flanks. When the gene is derepressed, these latter nucleosomes adopt a more open conformation and so do two of the nucleosomes positioned on the 5' flank. A dramatic change occurs in the 3' flank, whose involvement in the structural transitions of chromatin upon gene activation is p…