Search results for "SILDENAFIL"

Sildenafil inhibits the ROS production by xanthine oxidase

2008

Xanthine oxidase (XO) catalyzes the hydroxylation of a wide variety of substrates, including purines, pirimidines, pterins d aldehydes, to acids1. At relatively high oxygen pressure, it generates reactive oxygen species (ROS) as superoxides and hydroxyl radicals. The XO, detected in endothelial and epithelial cell outer surface, has been involved in ischemia/reperfusion injury1,2. Furthermore, XO-ROS production has been implicated in chronic hearth failure, inflammatory diseases, LDL oxidation, atherosclerosis, hypertension, cancer, aging1. Allopurinol, a hypoxanthine analogue developed as xanthine oxidase inhibitor 30 years ago, and oxypurinol, its oxidation product, have proved to be effe…

Utilizzo di Sildenafil citrato nella prevenzione della fibrosi dei corpi cavernosi nella IPP e nell'invecchiamento.

2011

Sildenafil protects epithelial cell through the inhibition of xanthine oxidase and the impairment of ROS production

2009

Recent reports suggest that xanthine oxidase (XO), a modified form of the native xanthine dehydrogenase enzyme, plays an important role in various forms of ischemic and vascular injuries, inflammatory diseases, and chronic heart failure. The XO inhibitors allopurinol and its oxidation product oxypurinol held considerable promises in the treatment of these conditions both in experimental animals and in human clinical trials. More recently, an endothelium-based protective effect of sildenafil, a well-known type-5 phosphodiesterase inhibitor, has been reported in preconditioning prior to ischemia/reperfusion in healthy human subjects. Based on the structural similarities between allopurinol an…

Alteraciones del ritmo del sueño en modelos animales de encefalopatia hepática

2016

Introducción: La encefalopatía hepática (EH) es un síndrome neuropsiquiátrico presente en pacientes con enfermedades hepáticas que conduce a diferentes alteraciones neurológicas que pueden llevar al coma y la muerte. Dentro de las manifestaciones neurológicas precoces de la enfermedad, están las alteraciones del sueño con una prevalencia casi del 50% en pacientes con cirrosis hepática sin evidencia clínica. En la actualidad, varios grupos de investigación han demostrado que el deterioro cognitivo y motor de los pacientes con enfermedades hepáticas se reproduce en modelos animales con EH e hiperamonémia sin fallo hepático, así que del mismo modo, estos deben reproducir las alteraciones del s…

Roflumilast N- oxide combined with sildenafil reverses cellular remodeling on IPF models

2017

Background: Idiopathic pulmonary fibrosis (IPF) is characterized by a rapid progressive lung decline and premature death after its diagnosis. Roflumilast displayed anti-fibrotic effects in animal and cellular models. Recent studies indicate that the combination of PDE4 and PDE5 inhibitors (sildenafil) potentiates anti-fibrotic properties of each drug, suggesting potential beneficts of this combination. Objectives: To study the effects from adding sildenafil to roflumilast N-oxide (RNO) inhibiting TGFβ1-induced human alveolar type II (AECII) epithelial-to-mesenchymal transition (EMT) and human fibrocyte to myofibroblast transition in vitro. Methods: AECII and fibrocytes were isolated from he…

Sildenafil protects human mammary epithelial cells against ROS production induced by estradiol

2010

Several studies suggest that xanthine dehydrogenase (XDH) and its oxidase form (XO) play an important role in various types of ischemic and vascular injuries. Recently, we have demonstrated that estradiol (E2) induces a significant decrease of the expression and activity of XDH and of its conversion to XO in human mammary epithelial cells. E2 is known to induce upregulation of eNOS gene expression in aortic endothelial cells. Because the XO-derived O2·- combines with ·NO to yield ONOO-, and considering that ONOO- converts XDH to XO, the resulting increase of XO activity and reactive oxygen species production would eventually lead to a further increase of ONOO- production, thus creating a vi…

Intracerebral haemorrhage associated with sildenafil citrate

2001

Sildenafil inhibits the proliferation of cultured human endothelial cells.

2013

The proliferation of endothelial cells plays a crucial role in the development of intraplaque angiogenesis (IPA). IPA is a major source of intraplaque hemorrhage and therefore contributes to the destabilization of atherosclerotic plaques. Therefore, the aim of the present study was to examine, whether sildenafil inhibits endothelial cell growth. The proliferation of human endothelial cells derived from umbilical cord veins (HUVEC) was examined on DNA level by measurements of (3H)-thymidine incorporation. Cell viability was analyzed using trypan blue staining. The proliferation of cultured human endothelial cells was significantly decreased by 1 μmol/l (-48.4%) and 10 μmol/l (-89.6%) sildena…

Intracerebral haemorrhage associated with sildenafil citrate

2001

Sildenafil citrate improves perinatal outcome in fetuses from pre-eclamptic rats

2012

Please cite this paper as: Herraiz S, Pellicer B., Serra V, Cauli O, Cortijo J, Felipo V, Pellicer A. Sildenafil citrate improves perinatal outcome in fetuses from pre-eclamptic rats. BJOG 2012;119:1394–1402. Objective  To evaluate perinatal outcome after sildenafil citrate (SC) administration at the onset of pregnancy in a rat pre-eclampsia model. Design  In vivo animal experimental study. Setting  Fundacion IVI-Instituto Universitario IVI, Valencia, Spain. Sample Control and pre-eclampsia-induced pregnant Wistar rats exposed to chronic SC administration. Methods  We evaluated the use of SC, which was tested as a potential therapeutic tool to maintain vasodilatation in complicated pregnanc…