Search results for "SINGLE"
showing 10 items of 4920 documents
Conformational Dynamics of the Dengue Virus Protease Revealed by Fluorescence Correlation and Single-Molecule FRET Studies.
2021
The dengue virus protease (DENV-PR) represents an attractive target for counteracting DENV infections. It is generally assumed that DENV-PR can exist in an open and a closed conformation and that active site directed ligands stabilize the closed state. While crystal structures of both the open and the closed conformation were successfully resolved, information about the prevalence of these conformations in solution remains elusive. Herein, we address the question of whether there is an equilibrium between different conformations in solution which can be influenced by addition of a competitive inhibitor. To this end, DENV-PR was statistically labeled by two dye molecules constituting a FRET …
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…
Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.
1992
Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.
The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells.
1998
SUMMARY Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral …
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme
1997
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…
Decoupled control scheme of grid-connected split-source inverters
2017
Grid-connected power conversion systems for renewable energy sources must fulfill several requirements, e.g., the high efficiency, the reduced cost and complexity, and, quite often, the boost capabilities that is usually achieved using a front-end dc–dc boost converter before the inversion stage, leading to a two-stage architecture. Meanwhile, single-stage power conversion systems, which perform the boosting operation within the inversion one, offer some potential advantages, in terms of reducing the complexity and the volume of the whole system. Among several proposed options, the split-source inverter (SSI) has been recently proposed by Abdelhakim et al. as an alternative option with some…
Wybory niekonkurencyjne w Polsce na poziomie lokalnym – wstępne oceny
2020
Artykuł podejmuje problem obsadzania mandatów bez głosowania w wyborach do rad gmin w gminach liczących do 20 tys. mieszkańców. W stosunku do wyborów z 2014 r. liczba takich przypadków wzrosła w 2018 r. o 77%. Wydaje się, że obowiązujące prawo wyborcze, w szczególności art. 418 § 1 (podział gmin na jednomandatowe okręgi wyborcze), art. 434 § 1 i 2 (zasady organizacji wyborów w przypadku zgłoszenia liczby kandydatów równej liczbie mandatów do obsadzenia) i art. 478 § 2 (tryb zgłaszania kandydatów na wójta lub burmistrza) Kodeksu wyborczego, w połączeniu z uwarunkowaniami lokalnej rywalizacji wyborczej, może sprzyjać powstawaniu zjawiska niekonkurencyjnych wyborów, które skutkują m.in. spadki…
Intimate relationship and its significance for eudaimonic well-being in young adults
2020
BackgroundPrevious studies indicate that being in a relationship has a beneficial effect on the physical and mental health and also significantly affects the quality of life. One of the essential elements of the relationship is intimacy, which includes sharing experiences, support and mutual understanding, as well as respect and care for the good of the partner. Being in a relationship, the nature of this relationship (marriage vs. informal relationship) as well as the level of intimacy and the assessment of the quality of the relationship may be in relation to the personal sense of well-being. In this research we intended to estimate the significance of these variables for the sense of wel…
Young adults in relationships and singles: religiosity and the structure of values
2020
The study aims to investigate the differences concerning the religiosity (understood as the placement of religious constructs in the structure of a personality) and value system of young adults who...
Cadmium Metal–Organic Frameworks Based on Ditopic Triazamacrocyclic Linkers: Unusual Structural Features and Selective CO 2 Capture
2017
International audience; Two three-dimensional cadmium metal organic frameworks with general formula [Cd-2(L-1)(H2O)(3)](NO3)(0.7)(HCOO)(0.2)Br-0.1 (Cd2L1, L-1 = 1,4,7-tris(4-carboxybenzy1)-1,4,7-triazacyclononane) and Cd(HL2)(H2O)(2) (CdL2, L-2 = 1,4,7-tris(3-(4-benzoate)prop-2-yn-1-yl)-1,4,7-triazacydononane) based on 1,4,7-triazacyclononane N-functionalized by different arylcarboxylic acids were prepared under solvothermal conditions and characterized by single crystal X-ray analysis and porosity measurements. The crystal structure of Cd2L1 reveals a cationic net with a bcs topology,. and nodes are constituted by dinuclear cadmium complexes, in which each cadmium atom adopts a hexacoordin…