Search results for "SOM"
showing 10 items of 8346 documents
Identification and molecular characterization of the high-affinity copper transporters family in Solanum lycopersicum
2021
Copper (Cu) plays a key role as cofactor in the plant proteins participating in essential cellular processes, such as electron transport and free radical scavenging. Despite high-affinity Cu transporters (COPTs) being key participants in Cu homeostasis maintenance, very little is known about COPTs in tomato (Solanum lycopersicum) even though it is the most consumed fruit worldwide and this crop is susceptible to suboptimal Cu conditions. In this study, a six-member family of COPT (SlCOPT1-6) was identified and characterized. SlCOPTs have a conserved architecture consisting of three transmembrane domains and β-strains. However, the presence of essential methionine residues, a methionine-enri…
Functional reconstitution of a proton-translocating system responsive to fusicoccin
1988
Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the fluorescence quenching of the dye 9-amino-6-chloro-2-methoxyacridine, markedly increased upon addition of fusicoccin to the reconstituted system. This effect could not be observed when binding sites and ATPase preparations were separately reconstituted into the proteoliposomes, thus demonstrating that fusicoccin binding to its receptor is a prerequisite for ATPase stimulation.
Anti-Inflammatory and Cytoprotective Effect of Plant Sterol and Galactooligosaccharides-Enriched Beverages in Caco-2 Cells
2019
Plant sterol (PS) (1 g/100 mL) enriched milk-based fruit beverages with or without galactooligosaccharides (GOS) (1.8 g/100 mL) were used in differentiated Caco-2 cells. Their potential cytopreventive effect against oxidative stress induced by cholesterol oxidation products (COPs) and their anti-inflammatory properties were evaluated. Preincubation (24 h) with bioaccessible fractions from beverages without and with GOS (MfB and MfB-G) completely prevented the COPs (60 μM/4 h) induced oxidative stress independent to GOS presence with exception to calcium influx and GSH content, where a partial protective effect was observed. Besides, MfB produced a significant (p < 0.05) reduction of IL-8 (4…
The Leiobunum rupestre species group: resolving the taxonomy of four widespread European taxa (Opiliones: Sclerosomatidae)
2016
Within the central European opilionid fauna the widely used species names Leiobunum rupestre Herbst, 1799 and Leiobunum tisciae Avram, 1968 pose taxonomic and distributional problems. In addition, Nelima apenninica Martens, 1969 is close to L. tisciae in terms of external and genital morphology, but is specifically distinct. While coxal denticulation is largely lacking in N. apenninica, the validity of the genus Nelima Roewer, 1910 is questioned again. In addition, Leiobunum subalpinum Komposch, 1998, a recently described novelty from the eastern Alps, is closely related to L. rupestre. The four species are combined as the morphologically defined Leiobunum rupestre species group. Except for…
DNA barcoding the phyllosoma of Scyllarides squammosus (H. Milne Edwards, 1837) (Decapoda: Achelata: Scyllaridae)
2016
Scyllarides has the largest number of species with commercial importance within the Scyllaridae family. As for other achelate lobsters, however, little is known of the unique long-lived planktonic phyllosoma stages of any of these tropical and temperate species. Recently, a large and diverse collection of Scyllaridae phyllosoma, compiled from cruises along the Coral Sea and spanning several years, has been analysed. Molecular evidence from DNA-barcoding and phylogenetic analyses is provided here on the identity of S. squammosus phyllosoma larvae, including stages that were previously undescribed or poorly known. As a consequence, the growth and morphological changes that occur during the mi…
Characterization and evolution of two bacteriome-inhabiting symbionts in cixiid planthoppers (Hemiptera: Fulgoromorpha: Pentastirini).
2009
International audience; Like other plant sap-sucking insects, planthoppers within the family Cixiidae (Insecta: Hemiptera: Fulgoromorpha) host a diversified microbiota. We report the identification and first molecular characterization of symbiotic bacteria in cixiid planthoppers (tribe: Pentastirini). Using universal eubacterial primers we first screened the eubacterial 16S rRNA sequences in Pentastiridius leporinus (Linnaeus) with PCR amplification, cloning, and restriction fragment analysis. We identified three main 16S rRNA sequences that corresponded to a Wolbachia bacterium, a plant pathogenic bacterium, and a novel gammaproteobacterial symbiont. A fourth bacterial species affiliated w…
EST based phylogenomics of Syndermata questions monophyly of Eurotatoria
2008
Abstract Background The metazoan taxon Syndermata comprising Rotifera (in the classical sense of Monogononta+Bdelloidea+Seisonidea) and Acanthocephala has raised several hypotheses connected to the phylogeny of these animal groups and the included subtaxa. While the monophyletic origin of Syndermata and Acanthocephala is well established based on morphological and molecular data, the phylogenetic position of Syndermata within Spiralia, the monophyletic origin of Monogononta, Bdelloidea, and Seisonidea and the acanthocephalan sister group are still a matter of debate. The comparison of the alternative hypotheses suggests that testing the phylogenetic validity of Eurotatoria (Monogononta+Bdel…
The tropical African genus Morgenia (Orthoptera, Tettigoniidae, Phaneropterinae) with emphasis on the spur at the mid tibia
2018
The authors revised the genus Morgenia Karsch, 1890 which now consists of eight species, of which three are here newly described (Morgeniaplurimaculata Massa &amp; Moulin, sp. n., M.angustipinnata Massa, sp. n., and M.lehmannorum Heller &amp; Massa, sp. n.). Six of the eight species occur in the Tri National Sangha (TNS) comprising Dzanga-Sangha Special Reserve and Dzanga Ndoki National Park (Central African Republic), whose high biodiversity has been recently highlighted. In particular the genus is characterised by the presence of a more or less long spur at the inner mid tibia, different in each species; in M.modulata, it moved lower down into a new position at about ¼ of tibia, w…
New conservation viewpoints when plants are viewed at one level higher. Integration of phylogeographic structure, niche modeling and genetic diversit…
2019
Protection and management of closely related endangered species and subspecies at a very narrow regional scale is the origin of multiple dysfunctional conservation decisions. These include artificially increased IUCN risk assessment categories and derived consequences: poor effectiveness in allocating public and private funds or repeat of unnecessary actions/facilities. Data provided by the revisited study of a group of W Mediterranean larkspurs (Delphinium ser. Fissa), including new data on demography, niche modeling, genetic diversity and phylogeography, contributed to a new and wider analysis of causes of threat. Although current IUCN Red List regulations did not allow for assessments at…
Pleurotus opuntiae revisited e An insight to the phylogeny of dimitic Pleurotus species with emphasis on the P. djamor complex
2018
Abstract The name Pleurotus opuntiae is indiscriminately used for describing mushrooms with white to off-white to white-grey pilei with short or absent stipe and dimitic hyphal system, which grow on plants of the genera Opuntia, Yucca, Agave, Phytolacca etc. However, the outcome of the present study evidences that this name should be reserved for specimens deriving from the Mediterranean area only; an epitype originating from Italy on Opuntia ficus-indica is designated. Pertinent material was sequenced by using the internal transcribed spacer region (ITS) and found to be phylogenetically related to P. djamor from Kenya and Nigeria, while members of the P. djamor complex from other continent…