Search results for "SPP"
showing 10 items of 178 documents
Molecular analysis ofPhytophthoradiversity in nursery-grown ornamental and fruit plants
2015
The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcoding approach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting the ITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa were detected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P. meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereas three phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P. nemorosa; and…
The ethnobotany, phytochemistry and biological properties of genus Ferulago – A review
2020
Abstract Ethnopharmacological relevance The genus Ferulago, belonging to the Apiaceae family, is found mainly in the Mediterranean area, Southwest and Middle Asia, the Caucasus and North Africa. Since ancient times, species of this genus have been largely employed in traditional medicine for their biological properties such as antimicrobial, anti-inflammatory, antispasmodic, insecticidal, and anti-malaria, cholinesterase inhibition effects, etc. Aims The scope of this paper is to present a comprehensive respect review of this interesting genus including traditional uses, chemical composition of volatile and non-volatile metabolites, and in vitro and in vivo biological properties either util…
MOLECULAR ANALYSIS OF PHYTOPHTHORA DIVERSITY IN ORNAMENTAL NURSERIES.
2013
A molecular approach based on the use of genus-specific nested-PCR primers (Scibetta et al., 2012; J. Microbiol. Meth. 88: 356-368) was utilized to detect Phytophthora species in soil and root samples of potted ornamentals, collected across Apulia and Calabria, Southern Italy. Analyzed samples comprised many plant species with different levels of symptoms of decline on the canopy and root rots. Extraction protocols were optimized to obtain DNA samples of appropriate quality from soil and roots. The analysis of sequences after cloning of nested-PCR amplicons enabled the identification of different Phytophthora species including P. nicotianae, P. cinnamomi, P. cryptogea, P. palmivora and P. n…
Identification of Phytophthora species infecting citrus in Vietnam
2013
In a survey aimed at identifying Phytophthora species infecting fruit crops in Viet Nam, P. citrophthora and P. nicotianae were the species most commonly recovered from soil and trees in citrus plantations. P. nicotianae was prevalent in citrus groves in the Ben Tre and Tien Giang provinces (southern Viet Nam). Interestingly, both A1 and A2 mating types of the latter species were found, while in other citrus-growing areas of the world, such as the Mediterranean region, South Africa, Australia and the Americas, A1 is the only mating type occurring in citrus groves, suggesting southern-east Asia could be a centre of origin of this Phytophthora species. Mixed infections of three Phytophthora s…
Whither the species? Phytophthora taxa, MOTUs and barcodes in the world of metagenomics
2015
The study of ecosystem diversity is being transformed by high throughput sequencing technology that allows an unparalleled depth of sampling of DNA barcode sequences. The opportunities are great at this fascinating interface of pathology, ecology, taxonomy and molecular biology but there are pitfalls. It is important to consider the potential for bias at all steps in the process from sampling through to data analysis. Increasingly Phytophthora diversity is being examined by metabarcoding of the PCR amplified rDNA ITS regions from soil, water or plant sample DNA. We have applied baiting and isolation, cloning and Sanger sequencing and Illumina MiSeq analysis to a time-series of filtered wate…
Celmeņu Armillaria spp. dzīvotspēja un patogenitāte Latvijas izcelsmes priežu Pinus sylvestris un egļu Picea abies stādos
2017
Celmeņu Armillaria spp. ģints sēņu izraisītā sakņu trupe ir iemesls ievērojamiem mežsaimnieciskajiem zaudējumiem. Darbā analizēta Armillaria solidipes, A. cepistipes un A. borealis dzīvotspēja, izplatīšanās potenciāls un patogenitāte 5-6 gadus vecos parasto priežu (563 gab.) un parasto egļu (350 gab.) stādos. Armillaria spp. inokulātu attīstība novērota stādu audzēšanai paredzētajā kūdras substrātā. Novērojumus priežu un egļu stādiem veica pēc attiecīgi 12 un 15 mēnešiem. Patogēna dzīvotspēja bija atkarīga no tā sugas: A. cepistipes būtiski augstāka, bet A. solidipes viszemākā. Augstāko izplatīšanās potenciālu konstatēja A. cepistipes, bet viszemāko A. solidipes. Visas trīs Armillaria sugas…
Celmeņu Armillaria spp. sastopamība parastās egles Picea abies audzēs kūdreņos
2022
Darbs izstrādāts Latvijas Valsts mežzinātnes institūta „Silava” Fitopatoloģijas un mikoloģijas laboratorijā un Ģenētisko resursu centrā. Pētījums veikts akciju sabiedrības „Latvijas valsts meži” un Latvijas valsts mežzinātnes institūta „Silava” zinātņietilpīgā līgumdarba „Sakņu trupes bojājumu risku mazināšana un tās ietekmes izpēte” ietvaros. Pētījumā analizēta celmeņu Armillaria spp. sastopamība ar Heterobasidion spp. inficētās parastās egles Picea abies audzēs ar nosusinātām kūdras augsnēm. Rizomorfu paraugi ievākti piecos parauglaukumos Meža pētīšanas stacijas „Kalsnava” teritorijā. Izmantojot programmu Win RHIZO 2005 C, analizēti rizomorfu morfoloģiskie rādītāji. Armillaria spp. sugu n…
The role of dominant tree cover and silvicultural practices on the postfire recovery of Mediterranean afforestations
2018
Fire is one of the major disturbance factors in Mediterranean-type ecosystems, where since long time man has deeply modified the natural fire regime. To know how woody species recover after fire is of prominent importance for understanding vegetation dynamics, as well as for the management of Mediterranean plantations, especially where broadleaved and coniferous trees coexist. Our research was carried out at Monte Petroso (Sicily), within an historical afforestation intervention in the Mediterranean basin. We assessed the post-fire response of mixed oaks and oak-pine afforestations within six experimental plots (two plots per homogeneous sector) differing in dominant tree species (Quercus i…
Vigna mungo, V. radiata and V. unguiculata plants sampled in different agronomical-ecological-climatic regions of India are nodulated by Bradyrhizobi…
2009
International audience; Vigna mungo, Vigna radiata and Vigna unguiculata are important legume crops cultivated in India, but little is known about the genetic resources in native rhizobia that nodulate these species. To identify these bacteria, a core collection of 76 slow-growing isolates was built from root nodules of V. mungo, V. radiata and V. unguiculata plants grown at different sites within three agro-ecological-climatic regions of India. The genetic diversity of the bacterial collection was assessed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified DNA fragments of the 16S–23S rDNA intergenic spacer (IGS) region, and the symbiotic genes nifH and nodC. One …