Search results for "SSRs"
showing 9 items of 9 documents
Identification and characterization of five polymorphic microsatellite loci in the freshwater copepod Hemidiaptomus gurneyi (Copepoda: Calanoida: Dia…
2016
Hemidiaptomus diaptomid copepods are known to be excellent biological indicators for the highly biodiverse crustacean communities inhabiting Mediterranean temporary ponds (MTPs), an endangered inland water habitat whose conservation is considered a priority according to the “Habitat Directive” of the European Union. This study reports on the characterization of five polymorphic microsatellite loci in Hemidiaptomus gurneyi, to be used as markers for fine-scale studies on the population genetic structure and metapopulation dynamics of a typical and obligate MTP dweller. The five selected loci proved to be polymorphic in the species, with three to five polymorphic loci per studied population. …
Mitochondrial simple sequenze repeats and 12s – rRNA gene reveal two distinct lineages of Crocidura russula (Mammalia, Sorcidae)
2004
A short segment (135 bp) of the control region and a partial sequence (394 bp) of the 12S-rRNA gene in the mitochondrial DNA of Crocidura russula were analyzed in order to test a previous hypothesis regarding the presence of a gene flow disruption in northern Africa. This breakpoint would have separated northeast-African C. russula populations from the European (plus the northwest-African) populations. The analysis was carried out on specimens from Tunisia (C. r. cf agilis), Sardinia (C. r. ichnusae), and Pantelleria (C. r. cossyrensis), and on C. r. russula from Spain and Belgium. Two C. russula lineages were identified; they both shared R2 tandem repeated motifs of the same length (12 bp)…
5.-6.-luokkalaisten oppilaiden sosiaaliset taidot ja kiusaaminen koulussa ja liikuntaharrastuksessa
2012
Tämän tutkimuksen tarkoituksena oli selvittää alakoulun 5.–6.-luokkalaisten oppilaiden sosiaalisia taitoja ja kiusaamista koulun ja liikuntaharrastuksen ympäristöissä. Mittasimme oppilaiden koulun ja liikuntaharrastuksen sosiaaliset taidot empatia-, yhteistyö- ja itsesäätelytaitojen osa-alueissa, jotka muodostettiin faktorianalyysillä Greshamin ja Elliottin SSRS-C –itsearviointilomakkeen pohjalta. Koehenkilöiksi valitsimme alakoulun viidennen ja kuudennen luokan oppilaita, joilla oli vähintään yksi liikuntaharrastus. Aineisto kerättiin Pohjois-Savossa ja Varsinais- Suomessa. Tutkimukseen valikoitui yhteensä 124 oppilasta, joista 70 olivat tyttöjä ja 54 olivat poikia. Ryhm…
Estimating the genetic diversity and structure ofQuercus trojanaWebb populations in Italy by SSRs: implications for management and conservation
2017
Studying the genetic diversity and structure of the current forest populations is essential for evaluating the ability to survive to future biotic and abiotic changes and planning conservation strategies. Quercus trojana is an eastern Mediterranean tree species with a fragmented distribution range, and its westernmost outposts are located in southern Italy. The demand for timber and cropland over the centuries has severely reduced it s occurrence in this part of the range. We assessed the genetic diversity and structure of the extant Italian populations of Q. trojana and derived conservation guidelines. A total of 322 samples were genotyped with six polymorphic nuclear microsatellite marker…
Does the genetic diversity among pubescent white oaks in southern Italy, Sicily and Sardinia islands support the current taxonomic classification?
2020
AbstractMolecular diversity analysis of deciduous pubescent oaks was conducted for populations from Calabria, Sicily and Sardinia. The aims of this study were twofold. First, to provide data on the genetic diversity of pubescent oaks from an understudied area which currently exhibits one of the highest concentrations of pubescent oak species in Europe. Second, to verify if these groups of oaks are genetically distinct and if their identification is in accordance with the current taxonomic classification. Molecular analyses of leaf material of 480 trees from seventeen populations belonging to putatively different pubescent oak species (Quercus amplifolia,Q. congesta,Q. dalechampii,Q. ichnusa…
Genetic Diversity of Major Olive Varieties from Southern Tunisia
2013
Considering the importance of olive-growing in Tunisia, microsatellite (SSR) analysis was used to study the genetic variation among twenty olive accessions from southern Tunisia. This set of olive microsatellites showed potential utility for genetic studies and it could contribute to the development of strategies for Tunisian germplasm conservation and breeding. Unweighted pair group method cluster analysis was performed and cultivars separated in three main groups. Five polymorphic simple sequence repeats (SSR) loci were employed and they revealed 38 alleles with a mean number of 7.6 alleles per locus. Genetic variability was wide as indicated by high values of both observed heterozygosity…
Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing
2012
The portunid crab Liocarcinus depurator is the dominant decapod crustacean present on mud bottoms of the continental shelf of the Mediterranean Sea. In order to better describe its genetic structure, a total of 11 microsatellites were isolated and tested in 28 L. depurator individuals from the Mediterranean locality of Tarragona. Initially, microsatellite isolation was carried out using the FIASCO methodology, but only 2 useful primer pairs could be designed. Given the low yield obtained, an alternative method based on 454 next-generation sequencing was assayed, which provided excellent results and from which another 9 primer pairs gave positive genotyping. All loci tested were polymorphic,…
Cytological and molecular characterization of three gametoclones of Citrus clementina
2013
Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (array-CGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a sing…
Data from: Genetic relationships, structure and parentage simulation among the olive tree (Olea europaea L. subsp. europaea) cultivated in Southern I…
2013
In this work, we assess both the morphological and genetic diversity of 68 important olive cultivars from three Southern Italian regions: Calabria, Campania and Sicily. Twenty-five phenotypic traits were evaluated and 12 simple sequence repeat (SSR) markers were analysed. All SSR primers were polymorphic and reliable. The total number of alleles per locus varied from 5 to 19 with an average number of 13.1 and a mean polymorphic information content (PIC) of 0.81. These results suggested high genetic diversity within these three olive germplasm collections. Morphological traits also showed significant variability amongst cultivars. Two cases of identity were found and ten statistically signif…