Search results for "Samples"
showing 10 items of 133 documents
Stable isotopes in monitoring terrestrial arthropods
2022
Monitoring of arthropods focuses typically on changes in population and range size over time. Yet, there are a myriad of other aspects that could and should be monitored under the ongoing global and local environmental change. Stable isotope analysis, widely employed in short-term ecological studies, has potential in long-term monitoring of arthropods. Here we discuss the use of stable isotopes in monitoring terrestrial arthropods, provide some empirical examples of the use of bulk tissue samples in stable isotope analysis, and outline future directions in using compound-specific stable isotope analysis in monitoring. We performed a literature search for 2012–2021 to see if stable isotopes …
Thermal and Mechanical Characterization of Yarn Samples from Flemish Tapestry of the Sixteenth Century
2022
We propose a physico-chemical approach for theharacterization of the conservation condition of yarns from a Flemish tapestry of the sixteenth century. The aging effect on the yarns’ performance was evaluated by comparison with commercial materials. Water uptake experiments highlighted the aptitude of yarns toward water sorption and their increased hydrophilicity upon aging. Thermogravimetric analysis can be considered a fast approach for the fiber identification and assessment on the material life-time. The dynamic mechanical analysis provided direct evidence on the yarns, conservation state and their performance under different mechanical stresses. The proposed characterization path can be…
A Novel Long-Term Graves’ Disease Animal Model Confirmed by Functional Thyrotropin Receptor Antibodies
2020
<b><i>Introduction:</i></b> A novel long-term murine model for Graves’ disease (GD) using repeated, long-term immunizations with recombinant adenovirus expressing the extracellular A-subunit of the human thyrotropin receptor (Ad-TSHR) was applied to evaluate the functional anti-TSHR-antibody (TSHR-Ab) profile. <b><i>Methods:</i></b> BALB/c mice received 7 immunizations with either 10<sup>10</sup> plaque-forming units of Ad-TSHR or control Ad-GFP. Naïve (nonimmuized native) mice were also studied. Three 3-weekly immunizations were followed by 4-weekly boosts until the 7th immunization. Blocking (TBAb) and stimulating (TSAb) TSHR-Ab …
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
2011
Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen (R) method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affec…
On the feasibility of dating the age of a nuclear incidental event by gamma-ray spectrometry of environmental samples
2019
The dating of a nuclear incidental event is an important element for the assessment of its enviromental impact and radiation protection implications on the involved population. In this framework, a procedure has already been proposed to determine the age of a nuclear event by measuring through a gamma-ray spectrometric analysis the activity of two fission products in a small sample of uranium irradiated for a short time in a nuclear reactor [1,2]. However, It may be useful to extend the application of the above mentioned procedure for assessing the “timing of occurrence” of an incidental event considering other samples and in particular, by sampling and measuring environmental samples. The …
Underdiagnosis Of Asthma And Associated Risk Factors In Italian General Population Samples
2017
Background: asthma underdiagnosis was reported in many recent studies, in particular in older subjects. Aim: to assess the risk factors associated with asthma underdiagnosis in two Italian general population samples. Methods: two general population samples living in Pisa (Central Italy, urban/suburban area) and Po Delta (North Italy, rural area) participated in some cross-sectional studies between 1980 and 2011. Through a questionnaire on health status and risk factors, Pisa sample was investigated in 1985-1988, 1991-1993 and 2009-2011; Po Delta sample was investigated in 1980-1982 and 1988-1991. Individuals reporting asthma symptoms or diagnosis in the previous studies were recently enroll…
Determination of Glyphosate and AMPA in Food Samples Using Membrane Extraction Technique for Analytes Preconcentration
2021
The method for determining glyphosate (NPG) and its metabolite AMPA (aminomethyl phosphonic acid) in solid food samples using UAE-SLM-HPLC–PDA technique was developed. Firstly, ultrasonic-assisted solvent extraction (UAE) and protein precipitation step were used for the analyte isolation. Then, the supernatant was evaporated to dryness and redissolved in distilled water (100 mL). The obtained solution was alkalized to pH 11 (with 1 M NaOH) and used directly as donor phase in SLM (supported liquid membrane) extraction. The SLM extraction was performed using 2 M NaCl (5 mL) as an acceptor phase. The flow rate of both phases (donor and acceptor) was set at 0.2 mL/min. The membrane extraction t…
Determination of Glyphosate and AMPA in Food Samples Using Membrane Extraction Technique for Analytes Preconcentration
2022
The method for determining glyphosate (NPG) and its metabolite AMPA (aminomethyl phosphonic acid) in solid food samples using UAE-SLM-HPLC–PDA technique was developed. Firstly, ultrasonic-assisted solvent extraction (UAE) and protein precipitation step were used for the analyte isolation. Then, the supernatant was evaporated to dryness and redissolved in distilled water (100 mL). The obtained solution was alkalized to pH 11 (with 1 M NaOH) and used directly as donor phase in SLM (supported liquid membrane) extraction. The SLM extraction was performed using 2 M NaCl (5 mL) as an acceptor phase. The flow rate of both phases (donor and acceptor) was set at 0.2 mL/min. The membrane extraction t…
"Voi ei, nää on tosi hyviä verrattuna muhun!" : uudenlaisen arviointimenetelmän toimivuus ranskan suullisen kielitaidon itsearvioinnissa
2013
ANALYTICAL PROCEDURES FOR THE DETERMINATION OF TRACE AMOUNTS OF XENOBIOTICS IN ENVIRONMENTAL WATER SAMPLES
2016
Wciąż wzrastające zanieczyszczenie środowiska związane z działalnością człowieka stanowi poważny problem ekologiczny współczesnego świata. Ksenobiotyki to substancje obce i potencjalnie szkodliwe dla organizmów żywych. Obecność niektórych z nich w środowisku wodnym może nieść za sobą poważne konsekwencje. Do najbardziej niebezpiecznych należą antybiotyki oraz pestycydy. Rozwój nauki i techniki umożliwił kontrolowanie tych substancji w środowisku naturalnym. Oznaczanie śladowych ilości ksenobiotyków w próbkach wód środowiskowych jest złożonym procesem, wymagającym zastosowania odpowiedniej metody ekstrakcyjnej i analitycznej. Złożoność matrycy oraz obecność substancji współobecnych stanowi d…