Search results for "Samples"
showing 10 items of 133 documents
Towards the potential use of (1)H NMR spectroscopy in urine samples for prostate cancer detection
2014
A simple method based in multivariate analysis of 1H NMR spectra profiles of urine samples can be used to detect patients with prostate cancer.
Toxoplasma gondii seroprevalence in extensively farmed wild boars (Sus scrofa) in Denmark
2019
Toxoplasma gondii is a zoonotic parasite of worldwide importance. In this study, we estimated T. gondii seroprevalence in extensively farmed wild boars in Denmark, where little is known about T. gondii in animal hosts. Our study focused on wild boars because they are considered good indicator species for the presence of T. gondii, and wild boar meat is used for human consumption. Serum samples from 101 wild boars collected in 2016-2018 from five different locations from the continental part of Denmark, Jutland, were screened for anti-T. gondii antibodies. The samples were analysed using a commercial indirect enzyme-linked immunosorbent assay (ELISA). Samples from 28 (27.7%) of the 101 wild …
Exposure to mercury among Spanish preschool children: Trend from birth to age four
2013
The purpose of this study is to describe the total hair mercury concentrations and their determinants in preschool Spanish children, as well as to explore the trend in mercury exposure from birth to the age four. This evolution has been scarcely studied in other birth cohort studies. The study population was 580 four year old children participating in the INMA (i.e. Childhood and Environment) birth cohort study in Valencia (2008–2009). Total mercury concentration at age four was measured in hair samples by atomic absorption spectrometry. Fish consumption and other covariates were obtained by questionnaire. Multivariate linear regression models were conducted in order to explore the associat…
Preliminary study on trace metal content in manna extracted from Fraxinus angustifolia cultivars in Madonie (Sicily, Italy)
2019
The manna is a product obtained from the elaborate sap solidification that comes out from the incisions made on the stem of some species of the genus Fraxinus L. In this study, we have analyzed 12 samples of manna to determine the chemical composition. Twenty elements (Al, As, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Rb, Sb, Se, Sr, U, V, Zn) were determined using an Inductively Coupled Plasma Mass Spectrometer. The results show that the most abundant elements are Fe > Rb > Al > Cu > Ba > Zn > Sr with median concentrations in the range of 0.9 – 10mg kg-1. The remaining elements exhibit median concentrations in the range of 0.0003 -0.5 mg kg-1. The manna also can be cons…
Femtosecond digital lensless holographic microscopy to image biological samples
2013
The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.
Importance of dogs as sentinels of West Nile Virus activity in urban and suburban areas
2012
Background: West Nile Virus (WNV) is a virus included in the Japanese encephalitis sero-complex within the genus Flavivirus. In August 2010, cases of West Nile disease were reported for the first time in Sicily. Neurological symptomswere observed in native horses resident in the rural areas around the province of Trapani, in the western part of the island. During the epidemic, important critical questions onwhen the viruswas introduced in the area and aboutwhether the virus had circulated/was circulating in theurban area, emerged and needed to be answered. A retrospective study using dog serum samples was designed to answer these questions. Methods: Between January 2009 and September 2010, …
P27-S Sex differences and cortical excitability in healthy adolescents
2019
Background Sex is known to influence brain development. However, sex differences in cortical excitability and their relationship to motor functions is unclear. Aim We examined the sex related differences in the degree of handedness, left and right motor cortex excitability and manual dexterity. Methods 28 healthy adolescents (age 16–19 years,19 girls) were studied. The degree of right-handedness was determined by the Waterloo Handedness Questionnaire. Motor threshold (MT) of the abductor pollicis brevis was measured on both hemispheres. Box and block test (BBT) was used for manual dexterity. Independent samples t-test was used in statistical analysis. Results There were no differences betwe…
A green method for the determination of cocaine in illicit samples
2013
Abstract Direct determination of cocaine in untreated seized samples has been made based on diffuse reflectance measurements of the near infrared (NIR) radiation through samples contained inside standard glass vials. The method used a series of previously analyzed samples, by the reference gas chromatography method, to build a partial least squares calibration model which was validated using an independent set of samples. The use of a general model for samples containing from 11.38% till 86.44% (w/w) cocaine was based on the use of spectral ranges from 12500.7 to 10128.6, 9339.8 to 6967.7 and 5388.3 to 4597.6 cm−1 with previous first derivative and vector normalization data pre-processing a…
R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies
2021
Next-generation sequencing (NGS) has enabled a deeper knowledge of the molecular landscape in non-small cell lung cancer (NSCLC), identifying a growing number of targetable molecular alterations in key genes. However, NGS profiling of liquid biopsies risk for false positive and false negative calls and parameters assessing the quality of NGS calls remains lacking. In this study, we have evaluated the positive percent agreement (PPA) between NGS and digital PCR calls when assessing EGFR mutation status using 85 plasma samples from 82 EGFR-positive NSCLC patients. According to our data, variant allele fraction (VAF) was significantly lower in discordant calls and the median of the absolute va…
Biomarker Analysis in Patients (pts) with Steroid-Refractory Acute Graft-Vs-Host Disease (aGVHD) Treated with Ruxolitinib (RUX) or Best Available The…
2020
BACKGROUND aGVHD, a common complication of allogeneic stem cell transplant (alloSCT), is driven by proinflammatory cytokines and chemokines that activate the immune system, resulting in end-organ damage. Steroids are first-line treatment but up to 50% of pts are steroid refractory (SR), resulting in high mortality and morbidity. RUX, a JAK1/JAK2 inhibitor, inhibits cytokine-dependent activation of the JAK-STAT pathway and cell proliferation and differentiation, which prevents worsening of aGVHD and allows recovery. JAK pathway inhibition by RUX may also lead to modulation of proinflammatory cytokines and prognostic GVHD biomarkers. The phase 3 randomized REACH2 trial (NCT02913261) in SR aGV…