Search results for "Sea Urchin embryos"
showing 8 items of 18 documents
Stress induced and physiological apoptosis during early development of sea urchin Paracentrotus lividus.
2008
Differences beetween physiological and induced apoptosis in sea urchin embryos.
2008
Vanadium perturbs the fertilization outcome and the metalloproteinase activity in sea urchin embryos
2021
Metal toxicology represents a current major topic due to the disper- sion of these elements in the environment. Metals are released from both natural sources and industrial activities. Some of them have also a clinical interest due to their application as metallodrugs (i.e., Pt, Cu, Au, Ru, and Y) or in medical diagnosis (Gd).1,2 Recently, V derivatives are considered as potential therapeutic factors in some diseases (e.g., obesity, diabetes, cancer, neurodegenerative and heart disorders). As a consequence, pharmaceutical residues could repre- sent emerging pollutants of aquatic environments, as wastewater treatment plants do not sufficiently remove these compounds3. Embryonic models repres…
Heavy Metals and Metalloids as Autophagy Inducing Agents: Focus on Cadmium and Arsenic
2012
In recent years, research on the autophagic process has greatly increased, invading the fields of biology and medicine. Several markers of the autophagic process have been discovered and various strategies have been reported studying this molecular process in different biological systems in both physiological and stress conditions. Furthermore, mechanisms of metalloid- or heavy metal-induced toxicity continue to be of interest given the ubiquitous nature and distribution of these contaminants in the environment where they often play the role of pollutants of numerous organisms. The aim of this review is a critical analysis and correlation of knowledge of autophagic mechanisms studied under …
Vanadium induces calcium depletion and cell selective apoptosis during development of sea urchin embryos
2021
Vanadium (V) is a metal widely distributed in soil, water and air. It has recently received growing interest because its compounds are often used in different applications, from industry to medicine.1 Here, using atomic absorption spectrometry, we demonstrate the predisposition of V to accumulate directly into embryonic cells, interfering with Ca uptake. At the morphological level, we observed dose- and time-dependent effects on phenotypes and on skeletal malformations. At the molecular level, V-exposed embryos showed the activation of the cellular stress response, inducing Hsp 60 and Hsp 70 synthesis and the activation of autophagy and apoptosis. The Hsps-mediated stress response to V appe…
Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects.
2008
Manganese (Mn), one of the most abundant metals in nature present in rocks, soil and water, is also found in soft bottom sediments of the oceans. It represents a trace element that is accumulated and utilized by all forms of life and plays multiple roles ranging from bone mineralization to cellular protection. Although Mn is an essential nutrient, exposure of cells/organisms to high levels of Mn cause toxicity. In the marine environment, increased concentrations of bio-available Mn often result from anthropogenic activities, and consequently, Mn represents a new important factor in environmental contamination. Emission of Mn into the marine environment occurs from metallurgic and chemical i…
Manganese-exposed embryos as blueprints to study signaling pathways involved in development
2012
Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos
2022
Metal pharmaceutical residues often represent emerging toxic pollutants of the aquatic environment, as wastewater treatment plants do not sufficiently remove these compounds. Recently, vanadium (V) derivatives have been considered as potential therapeutic factors in several diseases, however, only limited information is available about their impact on aquatic environments. This study used sea urchin embryos (Paracentrotus lividus) to test V toxicity, as it is known they are sensitive to V doses from environmentally relevant to very cytotoxic levels (50 nM; 100 nM; 500 nM; 1 µM; 50 µM; 100 µM; 500 µM; and 1 mM). We used two approaches: The fertilization test (FT) and …