Search results for "Sensory Organs"

Plasmalogens and cell‐cell communication between retinal glial cells

2017

National audience; Purpose Plasmalogens are glycerophospholipids containing a vinyl‐ether bond at sn‐1 position of their glycerol backbone and polyunsaturated fatty acids (PUFAs) at sn‐2. We have previously shown that plasmalogens are involved in the regulation of perinatal retinal vascular development and particularly in astrocyte template formation (Saab et al, PLoSONE 2012 9(6):e101076). Since retinal Müller cells and astrocytes can communicate through calcium waves and connexin 43‐rich gap junctions, the aim of our study was to determine whether a reduction of plasmalogen levels affects communication between retinal glial cells. Methods Primary Müller cells and astrocyte were isolated f…

Oxysterols: Influence on plasma membrane rafts microdomains and development of ocular diseases

2015

Oxidation of cholesterol into oxysterols is a major way of elimination of cholesterol from the liver and extrahepatic tissues, including the brain and the retina. Oxysterols are involved in various cellular processes. Numerous links have been established between oxysterols and several disorders such as neurodegenerative pathologies, retinopathies and atherosclerosis. Different components of the lipid layer such as sphingolipids, sterols and proteins participate to membrane fluidity and forme lipid rafts microdomains. Few data are available on the links between lipids rafts and oxysterols. The purpose of this review is to suggest the potential role of lipid rafts microdomains in the developm…

Apprehending ganglioside diversity: a comprehensive methodological approach

2015

Gangliosides make a wide family of glycosphingolipids ubiquitously expressed in mammalian tissues and particularly abundant in the brain and nervous system. They exhibit a huge diversity due to structural variations in both their oligosaccharidic chain and ceramide moiety, which represent a real analytical challenge. Since their discovery in the 1940s, methods have persistently improved until the emergence of Liquid Chromatography/Mass Spectrometry (LC/MS) which offers a high level of specificity and sensitivity and is suitable with high-throughput profiling studies. We describe here a comprehensive approach relying on various techniques and aiming at fully characterizing gangliosides in bi…

Acides gras polyinsaturés de la rétine : ont-ils une origine alimentaire ? Lipid Nutri+

2010

National audience; L’objectif du présent article est de faire le point sur l’origine des acides gras polyinsaturés dans la rétine neurale, en se focalisant en particulier sur le DHA. Nous appuierons notre propos en particulier sur nos données récentes obtenues sur des sujets décédés chez qui la rétine neurale et le couple EPR/choriocapillaire ont été prélevés et analysés pour leur contenu en lipides. Le profil en acides gras du tissu adipeux nous a servi de marqueur de consommation. Nous avons ainsi pu rechercher si les teneurs en AGPI de la rétine neurale sont le reflet des habitudes alimentaires des sujets.

Cortical Areas Involved in Vertical Saccades With a 3 Teslas Functional Mri

2009

Glaucoma epidemiology in Europe

2016

L’innovation dans le glaucome

2016

Activation of a caspase-3-independent mode of cell death associated with lysosomal destabilization in cultured human retinal pigment epithelial cells…

2008

International audience; Purpose: To characterize the possible cytotoxic effects of oxysterols (7-hydroxycholesterol (7-OH), 25-hydroxycholesterol (25-OH)) in human retinal pigment epithelial cells (ARPE-19) and to detail the relationships between some of these effects. Methods: ARPE-19 cells were treated with 7-OH and 25-OH. Cell viability was measured with the MTT assay. Membrane permeability, mitochondrial potential, and lysosomal integrity were measured by flow cytometry with propidium iodide, DiOC6(3), and acridine orange, respectively. Cell death was characterized by staining with Hoechst 33342, transmission electron microscopy, and analysis of the DNA fragmentation pattern. Caspase ac…

Is spectral domain optical coherence tomography useful in improving the contour line with the heidelberg retina tomograph?

2012

Purpose: To compare the measurements of the optic disc in glaucoma patients and glaucoma suspects with spectral domain optical coherence tomography (SD-OCT) and confocal scanning laser ophthalmoscopy (HRT) before and after adjustment of the contour line with the help of the OCT.Methods: In 109 consecutive patients (109 eyes), optic disc parameters were compared prospectively between the 2 devices before and after adjustment of the optic disc area (ODA) on the HRT3 with ODA obtained with the Cirrus SD-OCT as a reference. Intraclass correlation coefficients (ICC) and the 95% limits of agreement (LoA) with Bland-Altman plots were used to compare the agreement of the pre and post-adjustment mea…

Treatment of macular oedema (excluding age-related macular degeneration (AMD))

2021

Macular oedema corresponds to an increase in macular thickness most often associated with a rupture of the hematoretinal barriers. Diagnosis is based on the notion of reduced vision, but above all on multimodal imaging in which optical coherence tomography (OCT) plays an essential role both for the initial diagnosis and for follow-up. The mechanisms involved in the occurrence of macular oedema are primarily vascular endothelial growth factor (VEGF) and cytokines regardless of the aetiology of the oedema. Treatment often involves a combination of local treatment - primarily intravitreal injections of anti-VEGF or corticosteroids - and, in certain situations, such as diabetes or uveitis, gene…