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Performance analysis of two immunochromatographic assays for the diagnosis of rotavirus infection

2017

Group A rotaviruses (RVAs) are the primary cause of acute gastroenteritis (AGE) in young children worldwide. Several commercial tests including latex agglutination, enzyme-linked assays (ELISA) and immunochromatographic tests (ICT) have been developed for the diagnosis of RVA infection. In the present study, the performance of two commercially available one-step chromatographic immunoassays, CerTest Rotavirus + Adenovirus (Biotec S.L, Zaragoza, Spain) and Vikia Rota-Adeno (bioMerieux SA, Lyon, France) were retrospectively evaluated using Real-time PCR as reference test. Re-testing by Real-time PCR of 2096 stool samples of children hospitalized with AGE previously screened by ICTs (1467 by C…

Male0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia Clinica030106 microbiologymedicine.disease_causeSensitivity and SpecificityChromatography AffinityRotavirus InfectionsAstrovirusSentitivity03 medical and health sciencesPredictive Value of TestsVirologyRotavirusGenotypemedicineHumansImmunochromatographic AssaysDiagnostic ErrorsRetrospective Studiesbiologybusiness.industryInfant NewbornInfantRotavirubiology.organism_classificationVirologyGastroenteritisLatex fixation testRotavirus infectionItalyChild PreschoolSpecificityNorovirusFemaleImmunochromatographybusinessViral loadJournal of Virological Methods
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