Search results for "Sepharose"

showing 3 items of 33 documents

Immobilization of <i>Saccharomyces cerevisiae</i> Cells to Protein G-Sepharose by Cell Wall Engineering

2003

In this work, we explored the possibility of using the targeting of a heterologous protein to the cell wall of <i>Saccharomyces cerevisiae</i>, by fusing it to a cell wall protein, to construct yeast strains whose cells display on their surface proteins that bind to a matrix, so as to achieve the immobilization of the whole cells. With this aim, we created a gene fusion that comprises the region responsible for attachment of a cell wall protein to the cell wall, and the IgG binding region of staphylococcal protein A, and expressed it in the <i>mnn1mnn9</i> strain of <i>S. cerevisiae</i>. The surface display of the protein A-Icwp fusion protein was positiv…

biologyPhysiologyChemistrySaccharomyces cerevisiaeHeterologousCell Biologybiology.organism_classificationApplied Microbiology and BiotechnologyBiochemistryMicrobiologyFusion proteinYeastSepharoseCell wallBiochemistryIgG bindingbiology.proteinProtein GBiotechnologyMicrobial Physiology
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Mesoscopic gel at low agarose concentration in water: a dynamic light scattering study

1995

Previous work in our laboratory has shown that at very low agarose concentration in water gelation still occurs within mutually disconnected, high concentration regions generated by spinodal demixing. The freely diffusing particles obtained in these conditions are studied in the present work by depolarized dynamic light scattering and probe diffusion experiments. These particles are found to behave as large (in fact, mesoscopic) polymer fibers entangled in a continuously rearranged mesh with scaling parameters typical of partially flexible, neutral chains. The present results allow specifying the notion of mesoscopic gelation. They also reveal that the same symmetry-breaking mechanism that …

chemistry.chemical_classificationMesoscopic physicsWork (thermodynamics)SpinodalLightMacromolecular SubstancesSepharoseDiffusionAnalytical chemistryBiophysicsPolymerBiophysical PhenomenaCondensed Matter::Soft Condensed Matterchemistry.chemical_compoundDynamic light scatteringchemistryChemical physicsPercolationScattering RadiationAgaroseGelsResearch ArticleBiophysical Journal
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Imaging of VSOP labeled stem cells in agarose phantoms with susceptibility weighted and T2* weighted MR Imaging at 3T: determination of the detection…

2013

Objectives This study aimed to evaluate the detectability of stem cells labeled with very small iron oxide particles (VSOP) at 3T with susceptibility weighted (SWI) and T2* weighted imaging as a methodological basis for subsequent examinations in a large animal stroke model (sheep). Materials and Methods We examined ovine mesenchymal stem cells labeled with VSOP in agarose layer phantoms. The experiments were performed in 2 different groups, with quantities of 0–100,000 labeled cells per layer. 15 different SWI- and T2*-weighted sequences and 3 RF coils were used. All measurements were carried out on a clinical 3T MRI. Images of Group A were analyzed by four radiologists blinded for the num…

medicine.medical_treatmentAnimal Typeslcsh:MedicineLarge AnimalsSignalFerric CompoundsDiagnostic Radiologychemistry.chemical_compoundModel OrganismsLimit of DetectionMolecular Cell BiologymedicineAnimalsParticle Sizelcsh:ScienceBiologyDetection limitMultidisciplinarySheepmedicine.diagnostic_testStaining and Labelingbusiness.industryChemistryPhantoms ImagingSepharoseStem Cellslcsh:RMagnetic resonance imagingMesenchymal Stem CellsStem-cell therapyVSOPAnimal ModelsMagnetic Resonance ImagingStrokeDisease Models AnimalAgaroseMedicinelcsh:QVeterinary ScienceStem cellCellular TypesT2 weightedNuclear medicinebusinessRadiologyBiomedical engineeringStem Cell TransplantationResearch ArticleDevelopmental BiologyPloS one
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