Search results for "Sequence"
showing 10 items of 4987 documents
Analysing Complex Life Sequence Data with Hidden Markov Modelling
2016
When analysing complex sequence data with multiple channels (dimensions) and long observation sequences, describing and visualizing the data can be a challenge. Hidden Markov models (HMMs) and their mixtures (MHMMs) offer a probabilistic model-based framework where the information in such data can be compressed into hidden states (general life stages) and clusters (general patterns in life courses). We studied two different approaches to analysing clustered life sequence data with sequence analysis (SA) and hidden Markov modelling. In the first approach we used SA clusters as fixed and estimated HMMs separately for each group. In the second approach we treated SA clusters as suggestive and …
Hierarchical structures in Complex Systems: from DNA to financial markets
2000
In this paper we discuss the concepts of short-range and long-range correlated stochastic processes and we investigate the presence of such variables in two model complex systems. The selected model systems are DNA sequences of complete genomes and financial time series of equities traded in a stock market. Specifically, by starting from our research results, we discuss the statistical properties of (i) coding and non-coding regions of DNA and (ii) equity returns and volatility in financial markets. The stylized facts about these variables are presented and discussed with a focus on the statistical tools already used and/or still needed to better characterize these model complex systems.
Partial thioamide scan on the lipopeptaibiotic trichogin GA IV. Effects on folding and bioactivity
2012
Backbone modification is a common chemical tool to control the conformation of linear peptides and to explore potentially useful effects on their biochemical and biophysical properties. The thioamide, ψ[CS-NH], group is a nearly isosteric structural mimic of the amide (peptide) functionality. In this paper, we describe the solution synthesis, chemical characterization, preferred conformation, and membrane and biological activities of three, carefully selected, peptide analogues of the lipopeptaibiotic [Leu11-OMe] trichogin GA IV. In each analogue, a single thioamide replacement was incorporated. Sequence positions near the N-terminus, at the center, and near the C-terminus were investigated…
Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk
2019
Web spiders connect silk proteins, so-called spidroins, into fibers of extraordinary toughness. The spidroin N-terminal domain (NTD) plays a pivotal role in this process: it polymerizes spidroins through a complex mechanism of dimerization. Here we analyze sequences of spidroin NTDs and find an unusually high content of the amino acid methionine. We simultaneously mutate all methionines present in the hydrophobic core of a spidroin NTD from a nursery web spider’s dragline silk to leucine. The mutated NTD is strongly stabilized and folds at the theoretical speed limit. The structure of the mutant is preserved, yet its ability to dimerize is substantially impaired. We find that side chains of…
The long arm of education? A 28-year perspective on the consequences of education for job quality and wellbeing over adulthood
2016
International audience
Psychometric Properties of the “Alcohol Consumption Consequences Evaluation” (ACCE) Scale for Young Spanish University Students
2020
Instruments that evaluate alcohol use consequences among young people do not consider the intensive alcohol consumption pattern that is so characteristic during these ages. Some of these instruments are even ineffective in the Spanish population. Hence the interest in developing an instrument more adapted to the reality of our young people. A total of 601 university students (35.9% male and 64.1% female) from 18 to 20 years old were recruited. All of them answered a total of 77 items obtained from the review of both the scientific literature and the different scales used to measure consequences derived from alcohol consumption. In addition, they completed the AUDIT and the Timeline Followba…
Construction stages of cable-stayed bridges with composite deck
2012
A cable-stayed bridge is built by following a construction sequence of different subsequent stages that imply changes of the geometric configuration, restraints and consequently stress and strain patterns. A study is presented about stay stressing sequences in cable-stayed bridges with composite deck for the case of construction made by the cantilever method. Initial cable forces are found through partial elastic schemes of construction stages. Different stay stressing procedures are implemented and a comparison between them is proposed in order to evaluate the differences in terms of deformed shape and internal force distribution of the deck and the pylon. Results can be useful to give pra…
Light induces expression of a dehydrin-encoding gene during seedling de-etiolation in sunflower (Helianthus annus L.)
2007
The effects of light quality on the expression of a sunflower dehydrin-encoding gene, HaDhn1, were studied during seedling de-etiolation. Seeds were germinated in the dark and, after 5 days, seedlings were maintained well watered and de-etiolated under different lights for 3, 6, 12, and 24h. Exposure to white light stimulated HaDhn1 transcript accumulation in the cotyledons of these seedlings, contrary to seedlings grown in the dark. HaDhn1 transcripts increased also treating plantlets with monochromatic lights, especially red light. The increase of HaDhn1 transcripts is provoked by the formation of the active form of phytochrome. Further experiments, performed saturating active phytochrome…
Environmental control on granular clinoforms of ancient carbonate shelves
2006
The purpose of this paper is to document the influence of depositional environments on shallow-water, low-relief clinoforms from the description of five ancient carbonate platforms: the Neoproterozoic (Namibia), Middle Jurassic (France), Lower Cretaceous (France), Upper Cretaceous (Oman) and Miocene (Turkey). These examples have been investigated on the basis of field observations. The clinoforms are described with reference to geometric and compositional attributes: declivity, shape, height, sedimentary structures, sediment fabric and components. The results show great variability in stratal geometry, declivity and facies distribution: (1) depositional profiles vary from exponential, to si…