Search results for "Sequencing"

Aerobic granular sludge: State of the art, applications, and new perspectives.

2018

The aerobic granular sludge (AGS) process has attracted significant interest over the last decade and is one of the most promising wastewater treatment technologies. AGS offers several advantages over conventional activated sludge (CAS) including excellent settling, and higher volumetric loading capacity. Because of porosity, concentration gradients develop and stratified aerobic, anoxic, and anaerobic layers develop throughout granule depth. This is the reason for simultaneous nutrient removal in a single tank. Aerobic granulation is influenced by many parameters including wastewater characteristics and operating conditions. Among these, the anaerobic upflow feeding strategy and a properly…

Taxidermal Preparations of an Extinct Sicilian Wolf Population (Canis lupus Linnaeus, 1758): Biological Studies and Restoration

2022

In last decades, biotechnologies have enabled a deep development of knowledge in all fields of life sciences, allowing the collection of a wide range of data. Furthermore, the relationship between scientific investigations and conservation actions of cultural heritage is increasingly consolidated and now it is one of the peculiar points both for sustainable restoration and use of the cultural asset, also evaluating the cultural assets as source of biological information. In this study, Sicilian wolf (Canis lupus) specimens conserved in Sicilian Natural Museums, become a source of biological information on Sicilian biodiversity, establishing specific conservative protocol developed cooperati…

Candida pararugosa isolation from the oral cavity of an Italian denture wearer

2004

Candida pararugosa was first isolated from human feces, but after the initial description no further recovery from humans has been reported. During a study on oral Candida colonization in denture wearers living in Palermo (Italy), we isolated C. pararugosa from a 61-year-old woman without signs of oral candidosis. This constitutes, to the authors' knowledge, the first isolation of C. pararugosa from the oral cavity. After six months, colonization by C. pararugosa persisted, suggesting that this species could be a component of the normal oral microbiota. The identification procedure we used could be useful in elucidating the epidemiology of C. pararugosa and for establishing its clinical sig…

DETECTION OF NEW GENES RESPONSIBLE OF FAMILIAL RECESSIVE HYPERCHOLESTEROLEMIA: PRELIMINARY DATA FROM AN EXOME SEQUENCING APPROACH

2014

Mucosal Neuroma Syndrome without mutations of the RET-protooncogene: A histologic study on a case, supported by molecular genetic analysis.

2010

Mucosal neuromas are nerve hamartomas of the digestive tract and larynx, usually observed in the setting of Multiple Endocrine Neoplasia type 2B (MEN2B), i.e. in the presence of typical mutations and in association with medullary thyroid carcinoma, pheochromocytoma and marfanoid habitus. Exceptionally, they arise without the accompanying mutations and endocrine tumors, and in this paper we are reporting a histologic study on a case lacking the specific mutations. The patient was an adolescent girl with marfanoid habitus, with a left-sided epidermal nevus of the neck, and a bulging left upper lip and cheek. The left side of her tongue was considerably enlarged and studded with multiple protr…

Effect of pH on biological phosphorus uptake.

2006

An anaerobic aerobic laboratory scale sequencing batch reactor (SBR) was operated to study the effect of pH on enhanced biological phosphorus removal. Seven steady states were achieved under different operating conditions. In all of them, a slight variation in the pH value was observed during anaerobic phase. However, pH rose significantly during aerobic phase. The increase observed was due to phosphorus uptake and carbon dioxide stripping. When pH was higher than 8.2-8.25 the phosphorus uptake rate clearly decreased. The capability of Activated Sludge Model No. 2d (ASM2d) and Biological Nutrient Removal Model No. 1 (BNRM1) to simulate experimental results was evaluated. Both models success…

High-Quality Whole-Genome Sequence of an Estradiol-Degrading Strain, Novosphingobium tardaugens NBRC 16725

2019

In this work we report the complete sequence and assembly of the estradiol-degrading bacterium Novosphingobium tardaugens NBRC 16725 genome into a single contig using the Pacific Biosciences RS II system.

Construction of Metatranscriptomic Libraries for 5′ End Sequencing of rRNAs for Microbiome Research

2021

Metatranscriptomic sequencing enables studying community-wide gene expression profiles of microbial samples and getting functional insight on their up- or downregulated pathways. However, shotgun sequencing is not the most efficient way to study expression of ribosomal RNA genes or to compare lot of samples in experimental setups. Here we describe an efficient primer-independent method for processing and barcoding libraries for directional sequencing of the 5' end region of the RNA. When applying size selection of the original RNA, the method forms an optimal solution for the simultaneous analysis of bacterial, archaeal, and eukaryotic rRNA diversity.

Structure, organization and expression of two clustered cuticle protein genes during the metamorphosis of an insect, Tenebrio molitor.

1998

A 4-kb DNA segment of Tenebrio molitor (Insecta, Coleoptera) genomic DNA containing two larval-pupal cuticular genes has been cloned and sequenced. These genes, transcribed in opposite directions, are related in DNA sequence and the proteins encoded are very similar. Each of them contains a single intron located inside the sequence encoding the signal peptide, and a conserved sequence at -200 bp from the mRNA start position. These similarities in sequence suggest that these genes have evolved by duplication followed by diversification and that they are members of a family of genes with a common ancestry. They are the first example of clustered genes in Tenebrio molitor.

Identification des gènes impliqués lors de l'établissement de Lactobacillus casei dans l'intestin et caractérisation de l'opéron LSEI_0219-0221

2014

In bacteria which are in direct contact with their environment, genes transcription and proteins synthesis are efficiently regulated at each change of environmental parameters to allow cell survival. For intestinal commensal bacteria, these regulations must also allow symbiotic interactions and colonization whose molecular mechanisms, so far little known, are probably related, among others, to the bacteria surface (molecules exposed and secreted…). Lactobacillus casei, a commensal bacterium, has about 330 predicted genes involved in the composition and functionality of the cell surface. To have a global view of the whole genes involved in the establishment of L. casei in the gut, a reverse …