Search results for "Signal Transduction"

showing 10 items of 2020 documents

Involvement of Free Calcium in Action of Cryptogein, a Proteinaceous Elicitor of Hypersensitive Reaction in Tobacco Cells

1995

Treatment of suspension-cultured tobacco (Nicotiana tabacum var Xanthi) cells with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea, induced a great stimulation of Ca2+ influx within the first minutes. Ca2+ influx is essential for the initiation of cryptogein-induced responses, since ethyleneglycol-bis([beta]-amino-ethyl ether)-N,N[prime]-tetraacetic acid or La3+, which block Ca2+ entrance, suppress cryptogein-induced responses such as extracellular alkalinization, active oxygen species, and phytoalexin production. Moreover, once initiated, these responses require sustained Ca2+ influx within the 1st h. A Ca2+ ionophore (A23187) was able to trigger an extracellular alkaliniz…

0106 biological sciencesPhysiologyNicotiana tabacumPlant Science01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciences[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsGeneticsExtracellularmedicineStaurosporineProtein phosphorylationComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesbiologyPhytophthora cryptogeaPhytoalexinbiology.organism_classificationElicitorCell biologyBiochemistrychemistrySignal transductionResearch Article010606 plant biology & botanymedicine.drugPlant Physiology
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Phosphoproteins Involved in the Signal Transduction of Cryptogein, an Elicitor of Defense Reactions in Tobacco

2000

We previously reported that the signal transduction of cryptogein, an elicitor of defense reactions in Nicotiana tabacum cells, involves upstream protein phosphorylation. In the present study, induction of these early physiological events was further investigated with inhibitors of protein phosphatase (PP), okadaïc acid, and calyculin A. Calyculin A mimicked the effects of cryptogein, inducing an influx of calcium, an extracellular alkalinization, and the production of active oxygen species (AOS), suggesting that during cryptogein signal transduction the balance between specific protein kinase (PK) and PP activities was modified. To identify the phosphorylated proteins that could be involv…

0106 biological sciencesPhysiologyPhosphataseBiology01 natural sciencesFungal Proteins03 medical and health scienceschemistry.chemical_compound[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]TobaccoPhosphoprotein Phosphatasesmedicine[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyStaurosporineProtein phosphorylationEnzyme InhibitorsPhosphorylationProtein Kinase InhibitorsComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesFungal proteinIon TransportAlgal ProteinsGeneral MedicinePhosphoproteinsElicitorPlants ToxicchemistryBiochemistryPhosphorylationCalciumSignal transductionAgronomy and Crop ScienceSignal Transduction010606 plant biology & botanyCalyculinmedicine.drugMolecular Plant-Microbe Interactions®
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The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 …

2008

Abstract The plant defense elicitor cryptogein triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco (Nicotiana tabacum) cells, but microscopic observations of cell responses related to these early events were lacking. We determined that internalization of the lipophilic dye FM4-64, which is a marker of endocytosis, is stimulated a few minutes after addition of cryptogein to tobacco Bright Yellow-2 (BY-2) cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein because a lipid transfer protein, which binds to the same receptor as cryptogein but without triggering signaling, does not increase endocytosis. To…

0106 biological sciencesPhysiologymedia_common.quotation_subjectPlant ScienceEndocytosis01 natural sciencesClathrin[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants geneticsCell membrane03 medical and health sciencesGeneticsmedicineDEFENSE DES PLANTESInternalization030304 developmental biologymedia_common0303 health sciencesNADPH oxidasebiologyReceptor-mediated endocytosisFORMES ACTIVES DE L'OXYGENECell biologyElicitormedicine.anatomical_structureREACTIONS DE DEFENSEbiology.proteinSignal transduction010606 plant biology & botanyPlant Physiology
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Nuclear protein kinases: still enigmatic components in plant cell signalling

2010

International audience; Plants constantly face changing conditions in their environment. Unravelling the transduction mechanisms from signal perception at the plasma membrane level down to gene expression in the nucleus is a fascinating challenge. Protein phosphorylation, catalysed by protein kinases, is one of the major posttranslational modifications involved in the specificity, kinetic(s) and intensity of a signal transduction pathway. Although commonly assumed, the involvement of nuclear protein kinases in signal transduction is often poorly characterized. In particular, both their regulation and mode of action remain to be elucidated and may lead to the unveiling of new original mechan…

0106 biological sciencesPhysiologyp38 mitogen-activated protein kinasesPROTEIN KINASENUCLEAR TRANSLOCATIONPlant ScienceBiology01 natural sciencesSecond Messenger Systems03 medical and health sciencesNCK1Protein phosphorylationNuclear proteinNUCLEUS030304 developmental biologyPROTEIN (DE)PHOSPHORYLATION0303 health sciencesGRB10SIGNAL TRANSDUCTIONNuclear ProteinsAutophagy-related protein 13PlantsCell biology[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyBiochemistryCDC37Mitogen-activated protein kinasebiology.proteinProtein Kinases010606 plant biology & botany
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Nitric oxide: comparative synthesis and signaling in animal and plant cells.

2001

Since its identification as an endothelium-derived relaxing factor in the 1980s, nitric oxide has become the source of intensive and exciting research in animals. Nitric oxide is now considered to be a widespread signaling molecule involved in the regulation of an impressive spectrum of mammalian cellular functions. Its diverse effects have been attributed to an ability to chemically react with dioxygen and its redox forms and with specific iron- and thiol-containing proteins. Moreover, the effects of nitric oxide are dependent on the dynamic regulation of its biosynthetic enzyme nitric oxide synthase. Recently, the role of nitric oxide in plants has received much attention. Plants not only…

0106 biological sciencesPlant ScienceNitric Oxide01 natural sciencesAconitaseRedoxNitric oxide03 medical and health scienceschemistry.chemical_compound[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyAnimals[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyCyclic GMP030304 developmental biologyAconitate HydrataseMammals0303 health sciencesAdenosine Diphosphate RibosebiologyPlantsPlant cellBiosynthetic enzymeNitric oxide synthasechemistryBiochemistrybiology.proteinSignal transductionNitric Oxide SynthaseReactive Oxygen SpeciesSalicylic AcidSalicylic acid010606 plant biology & botanySignal TransductionTrends in plant science
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An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells

2008

International audience; The pathogenicity of various Streptomyces scabies isolates involved in potato scab disease was correlated with the production of thaxtomin A. Since calcium is known as an essential second messenger associated with pathogen-induced plant responses and cell death, it was investigated whether thaxtomin A could induce a Ca 2+ influx related to cell death and to other putative plant responses using Arabidopsis thaliana suspension cells, which is a convenient model to study plant–microbe interactions. A. thaliana cells were treated with micromolar concentrations of thaxto-min A. Cell death was quantified and ion flux variations were analysed from electrophysiological measu…

0106 biological sciencesProgrammed cell death[SDV.BIO]Life Sciences [q-bio]/BiotechnologyPhysiologyCellchemistry.chemical_element[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]Plant ScienceBiologyCalcium01 natural sciences03 medical and health sciences[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN][SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]medicineArabidopsis thaliana[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM][SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis030304 developmental biology0303 health sciencesVoltage-dependent calcium channelfood and beverages[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN]Streptomyces scabiesbiology.organism_classificationplant pathogenStreptomyces[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM][SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breedingcell deaththaxtomin A[CHIM.POLY]Chemical Sciences/Polymersmedicine.anatomical_structureBiochemistrychemistryion channelSecond messenger systemCalciumSignal transduction010606 plant biology & botanyJournal of Experimental Botany
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Quantitative proteomics reveals a dynamic association of proteins to detergent-resistant membranes upon elicitor signaling in tobacco.

2009

International audience; A large body of evidence from the past decade supports the existence, in membrane from animal and yeast cells, of functional microdomains playing important roles in protein sorting, signal transduction, or infection by pathogens. In plants, as previously observed for animal microdomains, detergent-resistant fractions, enriched in sphingolipids and sterols, were isolated from plasma membrane. A characterization of their proteic content revealed their enrichment in proteins involved in signaling and response to biotic and abiotic stress and cell trafficking suggesting that these domains were likely to be involved in such physiological processes. In the present study, w…

0106 biological sciencesProteomicsGTPase-activating proteinQuantitative proteomicsDetergentsPlasma protein bindingBiologymedicine.disease_causeProteomics01 natural sciencesBiochemistryMass SpectrometryAnalytical ChemistryCell membraneFungal Proteins03 medical and health sciencesProtein targetingTobaccomedicine[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyMolecular Biology030304 developmental biologyPlant Proteins0303 health sciencesFungal proteinStaining and LabelingResearchAlgal ProteinsCell MembraneCell biologymedicine.anatomical_structureBiochemistryLuminescent MeasurementsSignal transductionPeptidesReactive Oxygen Species010606 plant biology & botanyProtein BindingSignal TransductionMolecularcellular proteomics : MCP
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Combined phosphate and nitrogen limitation generates a nutrient stress transcriptome favorable for arbuscular mycorrhizal symbiosis in M edicago trun…

2013

International audience; Arbuscular mycorrhizal (AM) symbiosis is stimulated by phosphorus (P) limitation and contributes to P and nitrogen (N) acquisition. However, the effects of combined P and N limitation on AM formation are largely unknown. Medicago truncatula plants were cultivated in the presence or absence of Rhizophagus irregularis (formerly Glomus intraradices) in P-limited (LP), N-limited (LN) or combined P- and N-limited (LPN) conditions, and compared with plants grown in sufficient P and N. The highest AM formation was observed in LPN, linked to systemic signaling by the plant nutrient status. Plant free phosphate concentrations were higher in LPN than in LP, as a result of cros…

0106 biological sciencesRhizophagus irregularisNitrogenPhysiologyPlant SciencePlant Roots01 natural sciencesPhosphatesPhosphorus metabolismTranscriptome03 medical and health scienceschemistry.chemical_compoundNutrientSymbiosisGene Expression Regulation PlantStress PhysiologicalMycorrhizaeMedicago truncatulaBotanyPlant defense against herbivory[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyPhosphate Transport ProteinsGlomeromycotaSymbiosisPlant Proteins030304 developmental biology2. Zero hunger0303 health sciencesbiologyTerpenesfungifood and beveragesPhosphorusPhosphatebiology.organism_classificationMedicago truncatulaErythritolchemistrySugar PhosphatesTranscriptomeSignal Transduction010606 plant biology & botanyNew Phytologist
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Nitric oxide signalling in plants: interplays with Ca2+ and protein kinase

2008

International audience; Much attention has been paid to nitric oxide (NO)research since its discovery as a physiological mediator of plant defence responses. In recent years, newer roles have been attributed to NO, ranging from root development to stomatal closure. The molecular mechanisms underlying NO action in plants are just begun to emerge. The currently available data illustrate that NO can directly influence the activity of target proteins through nitrosylation and has the capacity to act as a Ca2+-mobilizing intracellular messenger. The interplay between NO and Ca2+ has important functional implications, expanding and enriching the possibilities for modulating transduction processes…

0106 biological sciencesSIGNALLINGPhysiologyPlant ScienceBiology01 natural sciencesNitric oxide03 medical and health sciencesTransduction (genetics)chemistry.chemical_compoundSNF-RELATED PROTEIN KINASE 2Mediator030304 developmental biology0303 health sciencesADP-RIBOSE CYCLIQUEPROTEIN KINASESKinaseCALCIUM 2+NitrosylationPlants[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/BotanicsNitric oxide metabolismCell biologySignallingBiochemistrychemistryCalciumIntracellularNITRIC OXIDE010606 plant biology & botanySignal Transduction
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From elicitins to lipid-transfer proteins: a new insight in cell signalling involved in plant defence mechanisms.

2002

Elicitins and lipid-transfer proteins are small cysteine-rich lipid-binding proteins secreted by oomycetes and plant cells, respectively, that share some structural and functional properties. In spite of intensive work on their structure and diversity at the protein and genetic levels, the precise biological roles of lipid-transfer proteins remains unclear, although the most recent data suggest a role in somatic embryogenesis, in the formation of protective surface layers and in defence against pathogens. By contrast, elicitins are known elicitors of plant defence, and recent work demonstrating that elicitins and lipid-transfer proteins share the same biological receptors gives a new perspe…

0106 biological sciencesSomatic embryogenesisProtein ConformationDefence mechanismsPlant ScienceBiology01 natural sciencesFungal Proteins03 medical and health sciencesErgosterolReceptor030304 developmental biologyPlant DiseasesPlant Proteins0303 health sciencesBinding proteinAlgal ProteinsLysophosphatidylcholinesProteinsElicitinAntigens PlantLipidsImmunity InnateBiochemistryOomycetesProtein-lipid complexStress MechanicalSignal transductionCarrier ProteinsPlant lipid transfer proteins010606 plant biology & botanySignal TransductionTrends in plant science
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