Search results for "Signal"

Comparative study assessing effects of sonic hedgehog and VEGF in a human co-culture model for bone vascularisation strategies.

2011

The morphogen sonic hedgehog (Shh) seems to mediate adult repair processes in bone regeneration and vascularisation. In this study we investigated the effects of Shh on co-cultures consisting of human primary osteoblasts and outgrowth endothelial cells in terms of angiogenic activation and vessel maturation in comparison to the treatment with the commonly used proangiogenic factor, VEGF. Both, stimulation with VEGF or Shh, leads to an increase in the formation of microvessel-like structures compared to untreated controls. In contrast to VEGF, proangiogenic effects by Shh could already be observed after 24 h of treatment. Nevertheless, after 14 days the angiogenic activity of OEC was compara…

Sonic hedgehog promotes angiogenesis and osteogenesis in a coculture system consisting of primary osteoblasts and outgrowth endothelial cells.

2009

A number of previous studies documented the angiogenic potential of outgrowth endothelial cells in vitro and in vivo and provided evidence that therapeutic success could depend on coculture or coimplantation strategies. Thus, deeper insight into the molecular mechanisms underlying this pro-angiogenic effect of cocultures might provide new translational options for tissue engineering and regenerative medicine. One promising signaling pathway in bone repair involved in neoangiogenesis and bone formation is the sonic hedgehog (Shh) pathway. In this article, we focus on the effect of Shh on the formation of microvessel-like structures and osteoblastic differentiation in cocultures of primary os…

VEGF and Notch Signaling in Angiogenesis

2015

The vascular system is responsible for providing every cell in vertebrate organisms with a sufficient supply of oxygen and nutrients, allowing waste disposal as well as transmitting immune responses among other functions. Thus, every tissue and organ requires an efficient network of blood vessels, which can be formed de novo (vasculogenesis) or from existing vessels (angiogenesis). The onset of the latter, namely endothelial cell (EC) sprouting, is the focus of this chapter. EC sprouting starts with the differentiation of ECs into guiding tip cells and proliferative stalk cells that form the growing sprout and it ends with the so-called anastomosis, when the sprout fuses with another sprout…

Transmembrane signalling mechanisms regulating expression of cationic amino acid transporters and inducible nitric oxide synthase in rat vascular smo…

1999

The signalling mechanisms involved in the induction of nitric oxide synthase and l-arginine transport were investigated in bacterial lipopolysaccharide (LPS)- and interferon-gamma (IFN-gamma)-stimulated rat cultured aortic smooth muscle cells (RASMCs). The expression profile of transcripts for cationic amino acid transporters (CATs) and their regulation by LPS and IFN-gamma were also examined. Control RASMCs expressed mRNA for CAT-1, CAT-2A and CAT-2B. Levels of all three transcripts were significantly elevated in activated cells. Stimulated CAT mRNA expression and l-arginine transport occurred independently of protein kinase C (PKC), protein tyrosine kinase (PTK) and p44/42 mitogen-activat…

A combined optical-microwave method to retrieve soil moisture over vegetated areas

2011

A simple approach for correcting for the effect of vegetation in the estimation of the surface soil moisture (wS) from L-band passive microwave observations is presented in this study. The approach is based on semi-empirical relationships between soil moisture and the polarized reflectivity including the effect of the vegetation optical depth which is parameterized as a function of the normalized vegetation difference index (NDVI). The method was tested against in situ measurements collected over a grass site from 2004 to 2007 (SMOSREX experiment). Two polarizations (horizontal/vertical) and five incidence angles (20◦, 30◦, 40◦, 50◦, and 60◦) were considered in the analysis. The best wS est…

Skeletons for parallel image processing: an overview of the SKiPPER project

2002

International audience; This paper is a general overview of the SKIPPER project, run at Blaise Pascal University between 1996 and 2002. The main goal of the SKIPPER project was to demonstrate the appli- cability of skeleton-based parallel programming techniques to the fast prototyping of reactive vision applications. This project has produced several versions of a full-fledged integrated pa- rallel programming environment (PPE). These PPEs have been used to implement realistic vi- sion applications, such as road following or vehicle tracking for assisted driving, on embedded parallel platforms embarked on semi-autonomous vehicles. All versions of SKIPPER share a common front-end and reperto…

Video Streaming Distribution in VANETs

2011

Streaming applications will rapidly develop and contribute a significant amount of traffic in the near future. A problem, scarcely addressed so far, is how to distribute video streaming traffic from one source to all nodes in an urban vehicular network. This problem significantly differs from previous work on broadcast and multicast in ad hoc networks because of the highly dynamic topology of vehicular networks and the strict delay requirements of streaming applications. We present a solution for intervehicular communications, called Streaming Urban Video (SUV), that 1) is fully distributed and dynamically adapts to topology changes, and 2) leverages the characteristics of streaming applica…

Velocity estimation in wideband mobile stations equipped with multiple antennas

2009

©2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE. Article also available from publisher: http://dx.doi.org/10.1109/VETECS.2009.5073907 A new method is proposed for estimating the velocity of wideband mobile stations (MSs) equipped with multiple antennas. The MS speed is determined using the well-known relationship between the direction-of-arrivals (DOAs) and the Doppler frequency shifts experienced by the mult…

Subcutaneous veins detection and backprojection method using Frangi vesselness filter

2015

Blood vessels detection is a common task performed in numerous medical procedures. During regular medical treatments venipuncture procedures are performed for invasive medication and blood sampling. Near infrared imaging technology can be used to visualize the subcutaneous veins in cases of difficult venous access. In this paper the methods for veins centerline detection and back projection is presented. In order to highlight the suitable veins for venipuncture, the centerline of larger veins are detected and back projected to the original image. The method is applied on the near infrared images of subjects selected from four different classes of skin tone. This can be helpful to medical st…

2021

Abstract Reliable patient-specific ventricular repolarization times (RTs) can identify regions of functional block or afterdepolarizations, indicating arrhythmogenic cardiac tissue and the risk of sudden cardiac death. Unipolar electrograms (UEs) record electric potentials, and the Wyatt method has been shown to be accurate for estimating RT from a UE. High-pass filtering is an important step in processing UEs, however, it is known to distort the T-wave phase of the UE, which may compromise the accuracy of the Wyatt method. The aim of this study was to examine the effects of high-pass filtering, and improve RT estimates derived from filtered UEs. We first generated a comprehensive set of UE…