Search results for "Single"

Production, crystallization and preliminary X-ray analysis of the human integrin alpha1 I domain.

1999

Integrin α1β1 is one of the main collagen receptors in many cell types. A fast large-scale production, purification and crystallization method for the integrin α1 I domain is reported here. The α1 I domain was crystallized using the vapour-diffusion method with a reservoir solution containing a mixture of PEG 4000, sodium acetate, glycerol and Tris–HCl buffer. The crystals beong to the C2 space group, with unit-cell parameters a = 74.5, b = 81.9, c = 37.3 Å, α = γ = 90.0, β = 90.8°. The crystals diffract to 2.0 Å and a 94.2% complete data set to 2.2 Å has been collected from a single crystal with an R merge of 5.8%.

Squaraine Dyes for Photodynamic Therapy: Mechanism of Cytotoxicity and DNA Damage Induced by Halogenated Squaraine Dyes Plus Light (>600 nm)¶

2004

Halogenated squaraine dyes 1 and 2 possess favorable photophysical and in vitro photobiological properties that make these new class of molecules interesting for photodynamic therapeutic applications. For a better understanding of the mechanism of their photobiological activity, we have analyzed the DNA damage and the cytotoxicity induced by these photosensitizers in mammalian cells and cell-free systems in the presence and absence of various additives and scavengers. Both photoactivated squaraines were found to be similar efficient in inducing single-strand breaks (SSB) in cell-free DNA when compared with the cellular DNA. Superoxide dismutase and catalase did not show any influence. Howev…

Tetra-urea calix[4]arenes 1,3-bridged at the narrow rim.

2009

The synthesis of special tetra-urea calix[4]arene derivatives is described. Two propyl ether groups in 1,3-position and a 5-iodo-isophthalamide bridge connecting two aminopropylether residues in 2,4-position at the narrow rim keep the molecule fixed in the cone conformation. The aryl urea residues are substituted by decyloxy groups in p-position to increase the solubility in apolar solvents, while the iodo substituent allows further functionalization. Two single crystal X-ray structures of 3 and 4 show a strongly pinched cone conformation in which the bridged phenol units are bent outwards, while the phenol units bearing the propyl ether groups are nearly parallel. The molecules are flexibl…

Lack of association betweenMDM2promoter SNP309 and clinical outcome in patients with neuroblastoma

2014

While a polymorphism located within the promoter region of the MDM2 proto-oncogene, SNP309 (T > G), has previously been associated with increased risk and aggressiveness of neuroblastoma and other tumor entities, a protective effect has also been reported in certain other cancers. In this study, we evaluated the association of MDM2 SNP309 with outcome in 496 patients with neuroblastoma and its effect on MDM2 expression. No significant difference in overall or event-free survival was observed among patients with neuroblastoma with or without MDM2 SNP309. The presence of SNP309 does not affect MDM2 expression in neuroblastoma. Pediatr Blood Cancer 2014; 61:1867–1870. © 2014 Wiley Periodicals,…

Blockchain and Smart Contracts: New Perspectives on Copyright Protection in the Digital Single Market

2022

New technologies making use of blockchains and smart contracts have been considered an efficient and innovative way to achieve the goal of effective copyright protection in the digital market. It is impossible to deny, however, that these innovative technologies raise serious questions about their compatibility with international, European, and national legislative frameworks, at least if we compare them with the original and most widely-used blockchain model (i.e., the so-called permissionless blockchain). Indeed, permissionless blockchains create a “law-free zone” resistant to any subsequent modification and judicial intervention. In this general context, this chapter aims to analyse the …

BOVITA: a first overview on genome-wide genetic diversity of Italian autochthonous cattle breeds

2017

The genetic heritage of Alpine local cattle breeds using genomic SNP data

2020

Abstract Background Assessment of genetic diversity and population structure provides important control metrics to avoid genetic erosion, inbreeding depression and crossbreeding between exotic and locally-adapted cattle breeds since these events can have deleterious consequences and eventually lead to extinction. Historically, the Alpine Arc represents an important pocket of cattle biodiversity with a large number of autochthonous breeds that provide a fundamental source of income for the entire regional economy. By using genotype data from medium-density single nucleotide polymorphism (SNP) arrays, we performed a genome-wide comparative study of 23 cattle populations from the Alpine Arc an…

On the origin and diversification of Podolian cattle breeds: testing scenarios of European colonization using genome-wide SNP data

2021

AbstractBackgroundDuring the Neolithic expansion, cattle accompanied humans and spread from their domestication centres to colonize the ancient world. In addition, European cattle occasionally intermingled with both indicine cattle and local aurochs resulting in an exclusive pattern of genetic diversity. Among the most ancient European cattle are breeds that belong to the so-called Podolian trunk, the history of which is still not well established. Here, we used genome-wide single nucleotide polymorphism (SNP) data on 806 individuals belonging to 36 breeds to reconstruct the origin and diversification of Podolian cattle and to provide a reliable scenario of the European colonization, throug…

Effectiveness and safety of electrical cardioversion for acute-onset atrial fibrillation in the emergency department: a real-world 10-year single cen…

2019

OBJECTIVE Despite limited evidence, electrical cardioversion of acute-onset atrial fibrillation (AAF) is widely performed in the emergency department (ED). The aim of this study was to describe the effectiveness and safety of electrical cardioversion of AAF performed by emergency physicians in the ED. METHODS All episodes of AAF electrically cardioverted in the ED were retrieved from the database for a 10-year period. Most patients not already receiving anticoagulants were given enoxaparin before the procedure (259/419). Procedural complications were recorded, and the patients were followed-up for 30 days for cardiovascular and hemorrhagic complications. RESULTS Four hundred nineteen eligib…

Instrument Characterization and First Application of the Single Particle Analysis and Sizing System (SPASS) for Atmospheric Aerosols

2005

We describe here the instrumental setup and first experiments with the mobile single particle analysis and sizing system (SPASS) for the on-line characterization of single atmospheric aerosol particles. Aerosols are introduced into the SPASS via a differentially pumped particle inlet system using an aerodynamic lens that forms a narrow particle beam. The particles are sized with a two-laser velocimeter and subsequently desorbed and ionized with a high-power pulsed Nd:YAG laser operating at 266 nm. Positive and negative ions formed are simultaneously detected in a bipolar time-of-flight mass spectrometer. Thus, the size and chemical composition of single aerosol particles can be characterize…