Search results for "Siphoviridae"
showing 3 items of 3 documents
Bias of library preparation for virome characterization in untreated and treated wastewaters
2020
The use of metagenomics for virome characterization and its implementation for wastewater analyses, including wastewater-based epidemiology, has increased in the last years. However, the lack of standardized methods can led to highly different results. The aim of this work was to analyze virome profiles in upstream and downstream wastewater samples collected from four wastewater treatment plants (WWTPs) using two different library preparation kits. Viral particles were enriched from wastewater concentrates using a filtration and nuclease digestion procedure prior to total nucleic acid (NA) extraction. Sequencing was performed using the ScriptSeq v2 RNA-Seq (LS) and the NEBNext Ultra II RNA …
The analysis of the oral DNA virome reveals which viruses are widespread and rare among healthy young adults in Valencia (Spain).
2017
We have analysed oral wash samples from 72 healthy young adults in Valencia (Spain) for a metagenomic analysis through the construction of shotgun libraries and high-throughput-sequencing. The oral viral communities have been taxonomically characterised as well as and the gene content from the latter. The majority of viruses are found in few individuals, with single occurrences being the most widespread ones, whereas universally distributed viruses, while present, are relatively rare, with bacteriophages from families Siphoviridae and Myoviridae, and Streptococcus phages, as well as the eukaryotic viral family Herpesviridae amongst the most widespread viruses. No significant differences wer…
Analysis of the therapeutical properties of a phage against Staphylococcus aureus pig isolates, and phage isolation against Pseudomonas aeruginosa fr…
2017
Antibioottiresistenttiys on kasvava ongelma, jonka selättämiseksi tarvitaan uusia keinoja. Prekliinisten tutkimustulosten perusteella bakteereja infektoivien virusten eli faagien on havaittu olevan hyvä vaihtoehto antibiooteille. Sairaalanäytteestä löydettyä metisilliinille resistenttiä Staphylococcus aureus (MRSA) -kantaa vastaan eristetty bakteriofagi karakterisoitiin sekvensoimalla ja läpäisyelektronimikroskoopilla, jotta sen mahdollista terapeuttista käyttöä voitaisiin selvittää. Faagilla havaittiin olevan keskikokoinen 43,098 bp:n pituinen genomi ja faagin morfologian perusteella se lukeutuu Siphoviridae-heimoon kuuluviin hännällisiin faageihin. Faagi puhdistettiin anioninvaihtokromato…