Search results for "Site-Specific"

Intragenomic recombination between homologous regions of genes II and IV promotes formation of bacteriophage f1 miniphages.

1987

Lactobacillus tucceti sp. nov., a new lactic acid bacterium isolated from sausage

2006

Abstract Following the application of several molecular techniques strain R 19c, isolated from sausage by Reuter in 1970 and deposited at the DSMZ as Lactobacillus sp., has been identified as pertaining to a new species. It showed singular ISR- Dde I and ISR- Hae III profiles that allowed its differentiation from 68 lactic acid bacteria reference strains analyzed. Phylogenetic analysis based on 16S rRNA gene sequences places this strain in the genus Lactobacillus within the Lactobacillus alimentarius group. Species L. versmoldensis is the closest phylogenetic neighbor with 96.3% sequence similarity. DNA–DNA hybridization experiments confirmed the independent status at species level of this …

Associations of LPL and APOC3 gene polymorphisms on plasma lipids in a mediterranean population: Interaction with tobacco smoking and the APOE locus

2002

We conducted a cross-sectional study in a Spanish population (n = 1,029) to investigate associations between the LPL and APOC3 gene loci (LPL-HindIII, LPL-S447X, and APOC3-SstI) and plasma lipid levels and their interaction with APOE polymorphisms and smoking. Carriers of the H− or the X447 allele had higher levels of HDL cholesterol (HDL-C), and lower levels of TG, after adjustment for age, body mass index, alcohol, smoking, exercise, and education (P < 0.01). The APOC3 polymorphism presented additive effects to the LPL variants on TG and HDL-C levels in men, and on TG in women. The most and the least favorable haplotype combinations were H−/X447/S1 and H+/S447/S2, respectively. These comb…

Bgl II restriction fragment length polymorphism of human complement C4A gene coincides with BF*F allele of factor B.

1988

Revitalization of a community site-specific art and art festivals : a case of art site Naoshima

2012

This thesis investigates public art and art festivals in the West, and a large scale art project in Japan, Benesse Art Site Naoshima, which is yet to be defined its place in the academic world. Through the case study of Benesse Art Site Naoshima, public art, site-specific art, art festivals, and a role of art museums are discussed. There are two parts to the thesis: (1) a brief survey of public art and site-specific art (of which has roots in Land art) and, (2) the case study of Art Site Naoshima which advocates the revitalization of rural communities through arts. The central aim of this thesis is to examine a wide range of art practices which is inspired not only by the topography of a ch…

Pulsed-field gel electrophoresis for the discrimination of Oenococcus oeni isolates from different wine-growing regions in Germany

2008

Reliable techniques are needed for the identification individual Oenococcus oeni strains with desirable flavor characteristics and to monitor the survival and contribution of inoculated and indigenous bacteria. Therefore, we investigated the suitability of pulsed-field gel electrophoresis (PFGE) for the discrimination of 65 O. oeni isolates from six different wine-producing regions in Germany. Among the restriction enzymes tested, genomic DNA digestions with Sfi I were most effective by displaying 56 (86%) different banding profiles. Our results underline the high capacity of PFGE for strain identification and differentiation. Cluster analysis of the DNA restriction patterns revealed no dis…

Effects of traditional coppice practices and microsite conditions on tree health in a European beech forest at its southernmost range

2016

Abstract: European beech (Fagus sylvatica) grows at the southern limit of its range in the mountain-Mediterranean vegetation belt up to the timberline. The southernmost beech forests of Sicily (southern Italy) show peculiar ecological, structural and silvicultural characteristics, growing in fragmented and isolated stands near the timberline and in topographically marginal unfavorable habitats. Past silvicultural practices increased the heterogeneity of stand structure at these sites. We compared stand structural characteristics and tree health in coppice-cut and control beech stands with respect to the local topographic gradient (bottom, slope and ridge) and canopy cover (clearing/border v…

iDamIDseq and iDEAR: an improved method and computational pipeline to profile chromatin-binding proteins

2016

DNA adenine methyltransferase identification (DamID) has emerged as an alternative method to profile protein-DNA interactions; however, critical issues limit its widespread applicability. Here, we present iDamIDseq, a protocol that improves specificity and sensitivity by inverting the steps DpnI-DpnII and adding steps that involve a phosphatase and exonuclease. To determine genome-wide protein-DNA interactions efficiently, we present the analysis tool iDEAR (iDamIDseq Enrichment Analysis with R). The combination of DamID and iDEAR permits the establishment of consistent profiles for transcription factors, even in transient assays, as we exemplify using the small teleost medaka (Oryzias lati…

ANIMA-L di Carla Sutera Sardo

2021

Il contributo prende in esame ANIMA-L, un progetto ideato da Carla Sutera Sardo nel 2020 e presentato nel contesto dell'Orto Botanico di Gibellina in occasione di Images Gibellina. Open Air and Site Specific Photo Festival. ANIMA-L declina in chiave contemporanea la relazione antichissima tra l'Uomo e la Natura. The contribution examines ANIMA-L, a project conceived by Carla Sutera Sardo in 2020 and presented in the context of the Botanical Garden of Gibellina on the occasion of Images Gibellina. Open Air and Site Specific Photo Festival. ANIMA-L interprets the ancient relationship between Man and Nature in a contemporary key.

The relative roles of intragenic polymorphisms of the vitamin d receptor gene in lumbar spine degeneration and bone density.

2001

Study Design. A retrospective cohort study. Objectives. To compare the magnitudes of the associations of TaqI polymorphisms of the vitamin D receptor gene with bone density and lumbar spine degeneration in the same sample. Summary of Background Data. Vitamin D receptor gene variations are associated with osteoporosis, osteoarthritis, and disc degeneration. Their role in these conditions remains poorly understood. Methods. Bone density of the spine and femur were determined through DEXA, and lumbar disc degeneration was determined from magnetic resonance imaging assessments of signal intensity, disc narrowing, bulging, anular tears, herniations, and osteophytes. Associations between these me…