Search results for "Small"

showing 10 items of 2441 documents

DECORIN EFFECTS ON PROTEOMIC PROFILING OF BREAST CANCER CELLS: AN UPDATED STUDY

2015

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells, among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect, the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules, the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin, belongs to the family of small leucine-rich proteoglycans (SLRP) and is involv…

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin belongs to the family of small leucine-rich proteoglycans (SLRP) and is involved physiologically in the fibrillogenesis of collagen. In the last few year a new anti-oncogenic role has been proposed for decorin1. This study aimed to implement the knowledge on the effects of ectopic decorin on breast cancer cells using as a reference point the results already achieved by our research group2 on the experimental model format. By breast cancer cell line 8701-BC and its transfected clone DEC-C2. The extension of the proteomic analysis combined with the mass spectrometry allowed to triplicate the number of identified proteins in our model. Among the newly identified proteins were members of the classes of metabolic enzymes S100 family and cell motility proteins which revealed a net decrease in the decorin transfected cells. Of considerable importance is the observation that these classes of proteins are the most involved in metastatic progression. These results confirm and reinforce the anti-oncogenic role hypothesized for decorin. The work was co-funded by the Italian 5x1000 to COBS.DECORIN
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Analyses of the TRACE V5 capability for the simulation of natural circulation and primary/containment coupling in BDBA condition typical of the MASLWR

2014

In the short term period the use of advanced Small Modular Reactor (SMR) is one of the most promising options for the deployment of nuclear technology. The validation and assessment of the best estimate thermal hydraulic system code TRACE against SMR thermal hydraulic phenomena is a novel effort. In this framework the use of the natural circulation database developed at the OSU-MASLWR test facility, simulating the MASLWR reactor prototype, is of interest for analyses of the TRACE code capability in predicting natural circulation and primary/containment coupled behavior in SMR. The target of this paper is to analyze the TRACE V5 capability for the simulation of natural circulation phenomena,…

Thermal hydraulicsEngineeringNatural circulationPrimary (chemistry)ContainmentCoupling (computer programming)business.industryNuclear engineeringMass flow ratebusinessSimulationTRACE (psycholinguistics)Small modular reactor
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Discovery and validation of small-molecule heat-shock protein 90 inhibitors through multimodality molecular imaging in living subjects.

2012

Up-regulation of the folding machinery of the heat-shock protein 90 (Hsp90) chaperone protein is crucial for cancer progression. The two Hsp90 isoforms (α and β) play different roles in response to chemotherapy. To identify isoform-selective inhibitors of Hsp90(α/β)/cochaperone p23 interactions, we developed a dual-luciferase (Renilla and Firefly) reporter system for high-throughput screening (HTS) and monitoring the efficacy of Hsp90 inhibitors in cell culture and live mice. HTS of a 30,176 small-molecule chemical library in cell culture identified a compound, N -(5-methylisoxazol-3-yl)-2-[4-(thiophen-2-yl)-6-(trifluoromethyl)pyrimidin-2-ylthio]acetamide (CP9), that binds to Hsp90(α/β) an…

Thymidine kinase activityProtein FoldingImmunoprecipitationLactams MacrocyclicBlotting WesternMice NudeThiophenesBiologyThioacetamideTritiumSmall Molecule LibrariesMiceco-chaperone p23Luciferases FireflyHeat shock proteinCell Line TumorNeoplasmsAcetamidesDrug DiscoveryBenzoquinonesAnimalsHumansImmunoprecipitationProtein IsoformsLuciferaseHSP90 Heat-Shock ProteinsLuciferases RenillaProstaglandin-E SynthasesMultidisciplinaryCell growthImidazolesbioluminescence imagingHsp90Small moleculeMolecular biologydrug developmentHigh-Throughput Screening Assayssmall-molecule inhibitorsIntramolecular OxidoreductasesLeadPNAS PlusCell culturePositron-Emission TomographyPyrazinesbiology.proteinPET/computed tomography imagingTomography X-Ray ComputedProceedings of the National Academy of Sciences of the United States of America
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Profilin1 regulates sternum development and endochondral bone formation.

2012

Bone development is a dynamic process that requires cell motility and morphological adaptation under the control of actin cytoskeleton. This actin cytoskeleton system is regulated by critical modulators including actin-binding proteins. Among them, profilin1 (Pfn1) is a key player to control actin fiber structure, and it is involved in a number of cellular activities such as migration. During the early phase of body development, skeletal stem cells and osteoblastic progenitor cells migrate to form initial rudiments for future skeletons. During this migration, these cells extend their process based on actin cytoskeletal rearrangement to locate themselves in an appropriate location within mic…

Time FactorsGenotypeMice Transgenicmacromolecular substancesBiologyTransfectionBiochemistryBone and BonesMiceProfilinsCell MovementOsteogenesisBone cellAnimalsProgenitor cellRNA Small InterferingCytoskeletonMolecular BiologyActinAllelesCytoskeletonMice KnockoutOsteoblastsMesenchymal stem cellGene Expression Regulation DevelopmentalCell migrationMesenchymal Stem CellsCell BiologyX-Ray MicrotomographyActin cytoskeletonCell biologyCartilageImmunologyNIH 3T3 CellsStem cellDevelopmental BiologyThe Journal of biological chemistry
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High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).

2011

14 páginas, 5 figuras, 3 tablas, S4 figuras, S2 tablas

Time FactorsScienceMolecular Sequence DataSequence DatabasesPlant ScienceBiologyDeep sequencingTranscriptomesRNA interferenceGene Expression Regulation PlantGenome Analysis ToolsOleaGene expressionmicroRNAGenome DatabasesPlant GenomicsGene silencingGene Regulatory NetworksGenome SequencingBiologyConserved SequenceGeneticsPlant Growth and DevelopmentMultidisciplinaryPolymorphism GeneticBase SequenceReverse Transcriptase Polymerase Chain ReactionSequence Analysis RNAGene Expression ProfilingQRRNAGene Expression Regulation DevelopmentalHigh-Throughput Nucleotide SequencingReproducibility of ResultsGenomicsOlive treesFunctional GenomicsRNA silencingMicroRNAsRNA PlantSmall MoleculesMedicineRNA InterferenceResearch ArticleBiotechnologyDevelopmental BiologyPloS one
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Tietoturvapolitiikan kehittäminen Pohjois-Pohjanmaan sairaanhoitopiirissä : Siponen & Puhakainen tietoturvapolitiikan kehittämismallin mukaisesti

2015

Tämän tutkimuksen tarkoituksena on selvittää, kuinka Siposen ja Puhakaisen tietoturvapolitiikan kehittämismallia toteutetaan ja kehitetään käytännössä. Se koostuu neljästä lähtökohdasta. Kirjallisuudesta ei löydy tietoturvapolitiikkaa, joka olisi toteutettu tämän mallin mukaisesti. Lisäksi tutkimuksessa selvitetään mallin soveltuvuutta Pohjois-Pohjanmaan sairaanhoitopiirissä (PPSHP). Tutkimus toteutettiin laadullisena toimintatutkimuksena, joka koostui viidestä vaiheesta: määrittäminen, suunnittelu, toteutus, arviointi sekä tarkentaminen ja oppiminen. Tutkimustietoaineisto kerättiin haastatteluiden avulla (PPSHP:n tietoturvasta ja tietosuojasta vastaavia henkilöitä), tutkimalla PPSHP:n stra…

Toimintatutkimustietoturvapolitiikan kehittämismallitietoturvapolitiikkaTietoturva
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Elucidating the aryl hydrocarbon receptor antagonism from a chemical-structural perspective.

2020

The aryl hydrocarbon receptor (AhR) plays an important role in several biological processes such as reproduction, immunity and homoeostasis. However, little is known on the chemical-structural and physicochemical features that influence the activity of AhR antagonistic modulators. In the present report, in vitro AhR antagonistic activity evaluations, based on a chemical-activated luciferase gene expression (AhR-CALUX) bioassay, and an extensive literature review were performed with the aim of constructing a structurally diverse database of contaminants and potentially toxic chemicals. Subsequently, QSAR models based on Linear Discriminant Analysis and Logistic Regression, as well as two tox…

ToxicophoreModels MolecularQuantitative structure–activity relationshipCell SurvivalRecombinant Fusion ProteinsQuantitative Structure-Activity RelationshipBioengineeringComputational biology01 natural sciencesSmall Molecule LibrariesCell Line TumorDrug DiscoveryCALUXBioassayAnimalsToxicologiaLuciferase GeneLuciferasesbiology010405 organic chemistryChemistryRobustness (evolution)Reproducibility of ResultsGeneral Medicinerespiratory systemAryl hydrocarbon receptor0104 chemical sciences010404 medicinal & biomolecular chemistryEstructura químicaReceptors Aryl Hydrocarbonbiology.proteinMolecular MedicineEnvironmental PollutantsAntagonismProteïnesSAR and QSAR in environmental research
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The demand for IPR services - to use or not to use a professional representative

2021

We analysed the use of professional representative services and related outcomes in patent, utility model, design right and trademark filings. We found that: 1) there is a positive association between the use of a professional representative and the likelihood of grant or registration; 2) the demand for professional intellectual property rights (IPR) services is heterogeneous between IPR types: applicants are most likely to hire professional representatives (IPR agents and attorneys) for patents, followed by utility models, design rights and trademarks; 3) individual applicants are less likely to use professional representatives compared to firm applicants. peerReviewed

Trademarkbusiness.industrytavaramerkitmallioikeusStrategy and ManagementvaltuutetutAccountinghyödyllisyysmallitIntellectual propertypatentitBusinessMarketingBusiness and International ManagementLawUtility modelInternational Journal of Intellectual Property Management
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IPR gender gaps: a first look at utility model, design right and trademark filings

2018

This study extends the analysis of the gender gap in patenting into design rights, utility model (UMs) and trademarks (TMs), which are complementary methods for protecting intellectual property. The analysis is descriptive and uses register data from the Finnish patent and registration office covering the years 1982–2013. A persistent gender gap is found for all intellectual property rights. The gap has narrowed over time for national patent, design right and TM filings but not for UM filings. It is found that, in comparison to men, women are relatively less often single inventors or designers and more often members of inventor or designer teams. peerReviewed

Trademarktavaramerkitpatentointi05 social sciencesGeneral Social ScienceshyödyllisyysmallitLibrary and Information SciencesIntellectual property050905 science studiesutility modelsukupuoliComputer Science ApplicationspatentmuotoiluRegister datatrademarkdesign rightpatentitBusinessGender gap0509 other social sciences050904 information & library sciencesUtility modelLaw and economicsScientometrics
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Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle

2015

The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution following a new biotin-based genomic run-on (BioGRO) technique. Compared with conventional profiling with chromatin immunoprecipitation, the analysis of the BioGRO profiles in Saccharomyces cerevisiae shows that RNA polymerase II has unique activity profiles at both gene ends, which are highly dependent on positioned nucleosomes. This is the first demonstration of the in vivo infl…

Transcription factoriesSaccharomyces cerevisiae ProteinsTranscription Elongation GeneticTranscription GeneticRNA polymerase II28Saccharomyces cerevisiaeBiology03 medical and health scienceschemistry.chemical_compoundTranscripció genèticaRNA polymeraseGeneticsRNA polymerase IRNA polymerase II holoenzyme9030304 developmental biologyGenetics0303 health sciencesGeneral transcription factorGene regulation Chromatin and Epigenetics030302 biochemistry & molecular biologyRNA Polymerase IIIGenomicsNucleosomesCell biologychemistryTranscription Termination Geneticbiology.proteinRNARNA Polymerase IIGenome FungalTranscription factor II DSmall nuclear RNA
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