Search results for "SoMe"
showing 10 items of 5114 documents
Proteomic analysis of extracellular vesicles shed in vitro by MDA MB 231 breast carcinoma cells
2010
Cross talk between tumor cells and connective tissue plays a key role in tumor progression. The communication is due to the release of signalling molecules from both tumor cells and surrounding stromal cells. Several secreted proteins lack the N-terminal signal peptides and, therefore, they are secreted by alternative unconventional processes such as secretion mechanism mediated by vesicle shedding in the extracellular matrix. Actually, a certain number of proteins, playing roles in some aspects of tumor progression, have been found in shed vesicles. For example, EMMPRIN, carried out in vesicles shed by tumor cells, stimulates matrix metalloproteinase (MMP) production in stromal fibroblasts…
Diversity of Isomerization Patterns and Protolytic Forms in Aminocarbene PdII and PtII Complexes Formed upon Addition of N,N′-Diphenylguanidine to Me…
2017
Reaction of the palladium(II) and platinum(II) isocyanide complexes cis-[MCl2(CNR)2] [M = Pd, R = C6H3(2,6-Me2) (Xyl), 2-Cl-6-MeC6H3, cyclohexyl (Cy), t-Bu, C(Me)2CH2(Me)3 (1,1,3,3-tetramethylbuth-1-yl abbreviated as tmbu); M = Pt, R = Xyl, 2-Cl-6-MeC6H3, Cy, t-Bu, and tmbu] with N,N′-diphenylguanidine (DPG) leads to DPG-derived metal-bound deprotonated acyclic diaminocarbene (ADC) species. This reaction occurs via a two-step process, involving the initial coupling of the guanidine with one of the isocyanides and leading to deprotonated monocarbene monochelated species, while the next addition grants the deprotonated bis-carbene bis-chelated metal compounds. DPG behaves as nucleophile, depr…
RAB18 Loss Interferes With Lipid Droplet Catabolism and Provokes Autophagy Network Adaptations
2020
Autophagy is dependent on appropriate lipid supply for autophagosome formation. The regulation of lipid acquisition and the autophagy network response to lipid-limiting conditions are mostly elusive. Here, we show that the knockout of the RAB GTPase RAB18 interferes with lipid droplet catabolism, causing an impaired fatty acid release. The resulting reduced lipid-droplet-derived lipid availability influences autophagy and provokes adaptive modifications of the autophagy network. These adjustments include increased expression and phosphorylation of ATG2B as well as augmented formation of the ATG12-ATG5 conjugate. Moreover, ATG9A shows an enhanced phosphorylation at amino acid residues tyrosi…
Inhibition of Rac1 signaling by lovastatin protects against anthracycline-induced cardiac toxicity
2011
Normal tissue damage limits the efficacy of anticancer therapy. For anthracyclines, the clinically most relevant adverse effect is cardiotoxicity. The mechanisms involved are poorly understood and putative cardioprotectants are controversially discussed. Here, we show that the lipid-lowering drug lovastatin protects rat H9c2 cardiomyoblasts from doxorubicin in vitro. Protection by lovastatin is related to inhibition of the Ras-homologous GTPase Rac1. It rests on a reduced formation of DNA double-strand breaks, resulting from the inhibition of topoisomerase II by doxorubicin. Doxorubicin transport and reactive oxygen species are not involved. Protection by lovastatin was confirmed in vivo. I…
Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.
2012
To investigate the potency of the topoisomerase II (topo II) poisons doxorubicin and etoposide to stimulate the DNA damage response (DDR), S139 phosphorylation of histone H2AX (γH2AX) was analyzed using rat cardiomyoblast cells (H9c2). Etoposide caused a dose-dependent increase in the γH2AX level as shown by Western blotting. By contrast, the doxorubicin response was bell-shaped with high doses failing to increase H2AX phosphorylation. Identical results were obtained by immunohistochemical analysis of γH2AX focus formation, comet assay-based DNA strand break analysis, and measuring the formation of the topo II-DNA cleavable complex. At low dose, doxorubicin activated ataxia telangiectasia m…
Potential-assisted deposition of mixed alkanethiol self-assembled monolayers
2010
Abstract Preparation of self-assembled monolayers (SAMs) usually involves passive incubation. The recently developed potential-assisted deposition is indeed more selective as well as 100-fold faster than passive adsorption, thereby enhancing the reproducibility of the monolayer deposition. This article aims to identify the electrodeposition conditions necessary to prepare mixed alkanethiol SAMs on gold surface. Parameters such as concentrations in solution, electrode polarization and deposition time were examined for two chain lengths, C 3 (mercaptopropionic acid, MPA) and C 18 (octadecanethiol, ODT). The kinetics and composition of the SAMs were systematically characterized by reductive st…
Ciclo del carbono y modelización biogeoquímica de un lago somero hipertrófico: la Albufera de Valencia
2015
Los lagos someros son uno de los ecosistemas acuáticos más numerosos a nivel global y hacen posible el desarrollo de multitud de actividades de interés económico y recreativo. En las últimas décadas, gran parte de estos lagos se han visto sujetos a un proceso de eutrofización cultural que a menudo conlleva una transición desde un estado "claro" caracterizado por una alta transparencia del agua y gran abundancia de macrófitos, a un estado "turbio" caracterizado por una baja transparencia del agua, dominancia de fitoplancton y macrófitos sumergidos prácticamente ausentes. El cambio que experimentó la Albufera de Valencia al estado turbio a principios de los años 1970 como resultado de un inte…
Late Activation of Stress-activated Protein Kinases/c-Jun N-terminal Kinases Triggered by Cisplatin-induced DNA Damage in Repair-defective Cells
2011
Although stress-activated protein kinases/c-Jun N-terminal kinases (SAPK/JNK) are rapidly activated by genotoxins, the role of DNA damage in this response is not well defined. Here we show that the SEK1/MKK4-mediated dual phosphorylation of SAPK/JNK (Thr-183/Tyr-185) correlates with the level of cisplatin-DNA adducts at late times (16–24 h) after drug treatment in both human and mouse cells. Transfection of platinated plasmid DNA also caused SAPK/JNK activation. A defect in transcription-coupled nucleotide excision repair resting on a mutation in Cockayne syndrome group B protein promoted the late SAPK/JNK activation following cisplatin exposure. Signaling to SAPK/JNK was accompanied by act…
Muscle and serum metabolomes are dysregulated in colon-26 tumor-bearing mice despite amelioration of cachexia with activin receptor type 2B ligand bl…
2019
Cancer-associated cachexia reduces survival, which has been attenuated by blocking the activin receptor type 2B (ACVR2B) ligands in mice. The purpose of this study was to unravel the underlying physiology and novel cachexia biomarkers by use of the colon-26 (C26) carcinoma model of cancer cachexia. Male BALB/c mice were subcutaneously inoculated with C26 cancer cells or vehicle control. Tumor-bearing mice were treated with vehicle (C26+PBS) or soluble ACVR2B either before (C26+sACVR/b) or before and after (C26+sACVR/c) tumor formation. Skeletal muscle and serum metabolomics analysis was conducted by gas chromatography-mass spectrometry. Cancer altered various biologically functional groups …
Sadarbības ekonomikas ietekme uz taksometru pārvadājumiem Eiropas Savienībā
2018
Sadarbības ekonomikas straujā ietekme uz tautsaimniecību Eiropas Savienībā ir radījusi vairākas problēmas, it īpaši pasažieru pārvadājumu jomā. Pasažieru pārvadātāji ar taksometriem Eiropas Savienības dalībvalstīs jūtas apdraudēti, jo autopārvadājumu tirgū ienāk jauni dalībnieki, kas uzņēmējdarbību veic uz sadarbības ekonomikas pamata. Eiropas Savienībā sadarbības ekonomika nav stingri regulēta, tādēļ ir grūtības saprast, cik stingri to regulēt un kādas sekas nākotnē dalībvalstu ekonomikās tas var izraisīt. Darba mērķis ir noskaidrot un analizēt sadarbības ekonomikas būtību, attīstību un tās ietekmi uz pasažieru pārvadājumiem ar taksometriem Eiropas Savienībā, noskaidrot, kādi regulējumi pa…