Search results for "Solanaceae"
showing 10 items of 51 documents
The Implication of Xyloglucan Endotransglucosylase/Hydrolase (XTHs) in Tomato Fruit Infection by Penicillium expansum Link. A
2007
In general, cell wall-degrading enzymes produced by plant pathogenic fungi are considered important pathogenicity factors. In this work, we evaluate the implication of xyloglucan endotransglucosylase/ hydrolase (XTHs), a potential hemicellulosic repairing enzyme, in the infection mechanism process by the fungus. This study investigated the SIXTHS expresion and xyloglucan endotransglucosylase (XET) activity during infection of two tomato fruit cultivars by Penicillium expansum Link. A. In infected fruits, XET specific activity decreased drastically after long infection periods, 24 and 48 h for Canario and Money Maker tomato fruits, respectively. Real Time RT-PCR of eleven SIXTHS also showed …
Modulation of Solanum tuberosum L. morphogenesis and antioxidative status in a stem explant culture by limitation of gas exchange: Putative effects o…
2000
Summary The main objective of the present study was to test the hypothesis that increased concentrations of ethylene under conditions of limited gas exchange during in vitro cultivation of potato ( Solanum tuberosum L.) stem explants may lead to increased capaciry of antioxidative defense. By varying the tightness of closure and application of inhibitors of ethylene biosynthesis it was possible to accumulate various concentrations of ethylene in the internal atmosphere of culture flasks, which depended on excision-stress induced ethylene biosynthesis. In tightly closed cultures inhibition of linear growth, promotion of radial expansion and development of an apical hook occurred. Treatment w…
Solanum lanceolatum (Solanaceae) in Sicily: A new alien species for the European flora
2015
Solanum lanceolatum Cav. (Solanaceae) is a species native to Central America (Mexico, Belize, Guatemala and Panama), that has been found naturalized near Sutera and Porto Empedocle (Sicily). This is the first record in Italy and Europe.
C7(P32) and C6(P34) PR proteins induced in tomato leaves by citrus exocortis viroid infection are chitinases
1990
[EN] Two chitinases induced in tomato leaves (Lycopersicon esculentum Mill cv. Rutgers) by citrus exocortis viroid (CEV) infection were purified. Their molecular masses determined by sodium dodecyl sulfate-gel electrophoresis were 32 kDa and 34 kDa and by filtration through Sephadex G-100 were 23 kDa and 25 kDa, respectively. These chitinases (P32 and P34) have been shown to be identical to the tomato pathogenesis-related proteins C7 and C6. They were purified in three stages: ammonium sulphate fractionation, chitin affinity chromatography and CM-Sepharose chromatography. The characterization of P32, the major component of the CEV-induced chitinase activity, revealed a basic protein (pI, 8·…
Sobre la presencia actual de "Atropa baetica" Willk. (Solanaceae) en la Alta Alcarria (Utande, Guadalajara)
2016
Se da noticia del hallazgo y presencia actual de "Atropa baetica" Willk. (Solanaceae) en una localidad de la Alta Alcarria, municipio de Utande (provincia de Guadalajara). Se indica la cuadrícula U.T.M. de 1×1 km donde se encuentra. Se presentan datos ecológicos y fitogeográficos básicos sobre su emplazamiento, algunos datos vegetativos descriptivos de los dos rodales o conjuntos de tallos encontrados y comentarios sobre posibles amenazas de la escueta población. Finalmente, se comenta la relación de esta nueva cita con referencias antiguas publicadas en la bibliografía.
Quantitation of Free and Glycosidically Bound Volatiles in and Effect of Glycosidase Addition on Three Tomato Varieties (Solanum lycopersicumL.)
2007
The volatile fractions of three tomato cultivars (p73, Jorge, and Durinta) were studied in both free and glycosidically bound forms. The possibility of increasing the concentration of free volatile compounds by adding selected glycosidases was also tested. The free volatile fraction (FVF) of tomato juice was directly determined by headspace solid-phase microextraction (SPME). To analyze the glycosidically bound fraction (GBF), tomato juice samples were extracted with C18 cartridges and the resulting glycoside extracts were enzymatically hydrolyzed. The released aglycons were determined by headspace SPME. Of these compounds, six were not previously reported to belong to the tomato GBF. The c…
Combining Hexanoic Acid Plant Priming with Bacillus thuringiensis Insecticidal Activity against Colorado Potato Beetle
2013
Interaction between insect herbivores and host plants can be modulated by endogenous and exogenous compounds present in the source of food and might be successfully exploited in Colorado potato beetle (CPB) pest management. Feeding tests with CPB larvae reared on three solanaceous plants (potato, eggplant and tomato) resulted in variable larval growth rates and differential susceptibility to Bacillus thuringiensis Cry3Aa toxin as a function of the host plant. An inverse correlation with toxicity was observed in Cry3Aa proteolytic patterns generated by CPB midgut brush-border membrane vesicles (BBMV) from Solanaceae-fed larvae, being the toxin most extensively proteolyzed on potato, followed…
Purification of hydroperoxide lyase from green bell pepper (Capsicum annuum L.) fruits for the generation of C6-aldehydes in vitro.
2002
The aim of this work was to compare the efficiency of different extracts of hydroperoxide lyase from green bell peppers in producing aldehydes: a crude extract, a chloroplastic fraction, and a purified enzyme were investigated. From a crude extract, the HPO lyase was purified by ion-exchange chromatography with a 22.3-fold increase in purification factor. Analysis by SDS-PAGE electrophoresis under denaturating conditions showed only one protein with a molecular weight of 55 kDa, whereas size-exclusion chromatography indicated a molecular weight of 170 kDa. A maximum of 7500 mg of aldehydes per g of protein was obtained with the purified enzyme within 20 min of bioconversion compared to 392 …
Sexual Dimorphism in Anthonomus santacruzi (Coleoptera: Curculionidae): a Biological Control Agent of Solanum mauritianum Scopoli (Solanaceae)
2020
AbstractThere is evident variation in body size amongst Anthonomus santacruzi Hustache, 1924, weevils. The aims of this study were to assess if the variation in body size in A. santacruzi weevils is a result of sexual dimorphism and what features can be used to distinguish males from females. The weevils were collected from field sites in Mpumalanga, South Africa, where they were introduced as biocontrol agents of Solanum mauritianum Scopoli. Body structures and the presence/absence of the tergal notch was examined under an optical stereomicroscope and SEM to assess differences between sexes. The morphometric analysis of the body structures included rostrum length (base–apex and antennal in…
Apple (Malus domestica) and Tomato (Lycopersicum esculentum) Fruits Cell-Wall Hemicelluloses and Xyloglucan Degradation during Penicillium expansum I…
2004
We characterized the changes in cell-wall hemicellulosic polysaccharides and the hemicellulose-degrading enzymes associated with apple and tomato fruits infected by Penicillium expansum. Our results showed a reduction in the molecular mass of hemicelluloses, with this reduction being particularly notable in the xyloglucan associated with P. expansum infection. The activation of fungal beta-glucanases was also highlighted. Fruit xyloglucan endotransglucosylase/hydrolase (XTH)-specific activity decreased drastically during the infection process in both apple and tomato fruits. We suggest that XTH reduction during the infection might be related with the fungus attack mechanism. We also suggest…