Search results for "Somatic embryogenesi"
showing 10 items of 42 documents
Factors affecting somatic embryogenesis in eight Italian grapevine cultivars and the genetic stability of embryo-derived regenerants as assessed by m…
2016
Abstract Embryogenic cultures have been used in cryopreservation, genetic transformation, propagation, virus elimination, induced mutagenesis and in many other biotechnological applications, providing excellent opportunities for biotechnology advances in grapevine. Unfortunately the efficiency of somatic embryogenesis (SE) is genotype-dependent in addition to showing interaction with explant type used and the plant growth regulator (PGR) composition. In order to identify the interaction of these parameters in SE, we tested eight wine grapevine cultivars, three explant types (ovary, anther/filament and stigma/style) and four PGR combinations in a statistically designed experiment. The genoty…
Micropropagation of juvenile and adultDigitalis obscura and cardenolide content of clonally propagated plants
1991
Cultures ofDigitalis obscura L. were established from axillary buds of mature plants or leaves of seedlings obtained under aseptic conditions. Explants were cultured on Murashige and Skoog medium containing benzyladenine and/or naphthaleneacetic acid. Shoot proliferation from axillary buds was not affected by seasonal fluctuations in the stock plants and increased relative to the cytokinin concentration, but auxin reduced the multiplication rate. Differentiation of somatic embryos and adventitious buds from cultured leaves required naphthaleneacetic acid alone or combined with benzyladenine, respectively. Cardenolide pattern and content of the regenerated plants were determined by high perf…
Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives
2019
Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advan…
Environmental and Biochemical Factors Controlling the in Vitro Emergence of Somatic Embryos in European Spindle Tree (Euonymus europaeus L)
2000
The European spindle tree (Euonymus europaeus L. Celastraceae) is a shrubby tree widely distributed in temperate regions of western European countries from the northern part of France to St. Petersburg and the western part of Russia. The Celastraceae comprises 55 genera, including at least 850 known species of trees and shrubs which are found in tropical and temperate regions. Various secondary metabolites are elaborated in the family including steroids, triterpenoids, sesquiterpene, peptide ore alkaloids. The spindle tree is commonly found in quickset hedges and small broad-leaved forests when the soil is deep, moist, clayey and chalky. The leaves are relatively small (3–4 cm long) simple …
Development of multicellular pollen of Eriobotrya japonica Lindl. through anther culture
2006
8 pages, 5 figures.-- Printed version published Dec 2006.
Correlating stress and somatic embryogenesis for NBT: myth or reality?
2018
Global warming and growing demography have increased demand for agronomic resources, leading to increasing lack of land suitable for agriculture and provoking several abiotic stresses which, added to biotic ones, result in physiological and metabolic disorders that impact on crop yield when most needed. Reducing this impact is a major scientific and agronomic challenge and biotechnology would be an efficient alternative. However, to reduce risks of somaclonal variation among regenerants it is better to produce them by somatic embryogenesis directly from explants or gametes, or indirectly from callus or cell suspensions. Thus, globular embryos regenerate and develop through to the heart, tor…
Genetic instability in calamondin (Citrus madurensis Lour.) plants derived from somatic embryogenesis induced by diphenylurea derivative
2007
Somatic embryos were regenerated in vitro from calamondin style-stigma explants cultured in the presence of N (6)-benzylaminopurine (BAP) cytokinin and three synthetic phenylurea derivatives, N-(2-chloro-4-pyridyl)-N-phenylurea (4-CPPU), N-phenyl-N'-benzothiazol-6-ylurea (PBU) and N,N'-bis-(2,3-methilendioxyphenyl)urea (2,3-MDPU). The phenylurea derivative compounds tested at micromolar level (12 muM) were able to induce a percentage of responsive explants significantly higher from that obtained with BAP and hormone-free (HF) conditions. In order to verify the genetic stability of the regenerants, 27 plants coming from different embryogenic events were randomly selected from each different …
Morphogenesis in leaf and single-cell cultures of mature Juniperus oxycedrus
1996
Single cells were mechanically isolated from leaf-derived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 micro M 2,4-dichlorophenoxyacetic acid and 100 mg l(-1) casein hydrolyzate. Although single-cell-derived callus showed poor morphogenic potential, both adventitious shoots and embryogenic tissues differentiated from the callus. We also achieved induction of somatic embryogenesis in leaf explants of mature J. oxycedrus trees cultured in the presence of 6.0 or 10.0 micro M 2,4-dichlorophenoxyacetic ac…