Search results for "Sorting"
showing 10 items of 187 documents
Experimental studies on the suitability of human mesothelial cells for seeding vascular prostheses: shear stress resistance in vitro
1996
This investigation forms part of a study on the suitability of human omentum mesothelial cells (HOMES) as an alternative to endothelial cells (EC) for seeding vascular grafts. Isolated HOMES were grown in primary culture and characterized by their morphology (light microscopy and scanning electron microscopy (SEM)), as well as by fluorescence-activated cell sorting (FACS) and immunocytochemistry. The latter two methods showed cells which were positive for smooth muscle-type actin and cytokeratin, but negative for factor VIII-related antigen. HOMES were grown to confluence on glass with or without a fibronectin coating. Controlled shear stress was applied for up to 30 min using a plate and c…
Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
2010
During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and char…
Physical Load and Preventive Measures in Metal Manufacturing Industry
2020
In today’s advanced work environment, workers are still exposed to ergonomic risks in the work environment and employees are exposed physically hard work that includes moving loads and frequent repetitive movements at fast pace. For the ergonomic risk analysis and workload assessment, three work operations with the high physical activity were selected: the welder’s workplace, the ironing board sorting line and the ironing board’s packing station. Subjective and objective ergonomics research methods were used. Research results show that employees in selected work operations are exposed to a fast work speed and often, monotonous movements at the sorting line and lifting heavy loads at the iro…
A Ge(Li)Ge(Li) sum-peak (summing coincidence) spectrometer
1970
Abstract The sum-peak spectrometer (also called the integral-bias summing coincidence spectrometer) arrangement earlier developed with NaI(Tl) detectors is extended to Ge(Li) detectors. The integral-bias method in sorting sums of coincident pulse amplitudes is replaced by a set of pulse-height selection windows, which simply and more effectively aid in the analysis of sum-peak complexities associated with the symmetric linear summing procedure employed. A large part of the original information lost in summing of the pulse amplitudes can be retained by simultaneous sorting of the total spectrum into suitable subgroups. The arrangement represents in effect a real-time totalizing spectrometer …
M10_Microfluidics_for_CNT
2018
Droplets are preferentially directed to the channel with higher flow rate in the passive sorting junction. When the droplets are exposed to DEP field, the DEP force pull them to the narrower channel.
M5_Microfluidics_for_CNT
2018
The features of the sorting junction. Pressure balancer helps to equilibrate the pressure difference created by the presence of a droplet. The transition from shallow to deep channel mediates the transition from free to confined regime. The droplets are separated in the sorting junction according to the flow rate ratio.
M8_Microfluidics_for_CNT
2018
A comparation of two sorting strategies. While the shallow channel guides the droplets more precisely to the sorting point, the confined droplets are more vulnerable to coalescence and break-up. The deep channel lowers the positioning precision, however, the droplets are more stable.
The Impact of Grain Size Composition and Organic Matter Content on Magnetic Susceptibility of Anthropogenically Transformed Bottom Sediments, as Exem…
2017
The article presents the results of an analysis of the surface layer of bottom sediments in the naval harbour in Hel. During field work conducted in June 2013, 54 sediment samples were collected. The aim of the study was to examine magnetic susceptibility of harbour sediment as a parameter determining the degree of anthropopressure. As part of laboratory tests, grain size analysis was performed in order to determine organic matter content and magnetic susceptibility of the sediment. The study also measured the impact of environmental factors on fluctuations in the other studied parameters. The results demonstrate increased dynamics of sedimentary environment in the open part of the naval ha…
Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry.
2008
TAP is responsible for transferring cytosolic peptides into the ER, where they can be loaded onto MHC molecules. Deletion of TAP results in a drastic reduction of MHC class I surface expression and alters the presented peptide pattern. This key molecule in antigen processing is tackled by several viruses and lost in some tumors, rendering the altered cells less vulnerable to T cell-based immune surveillance. Using the TAP-deficient cell line LCL721.174 and its TAP-expressing progenitor cell line LCL721.45, we identified and quantified more than 160 HLA ligands, 50 of which were presented TAP-independently. Peptides which were predominantly presented on the TAP-deficient LCL721.174 cell line…
Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation
1995
Processing of the hepatitis C virus polyprotein is mediated by host cell signalases and at least two virally encoded proteinases. Of these, the serine-type proteinase encompassing the amino-terminal one-third of NS3 is responsible for cleavage at the four sites carboxy terminal of NS3. The activity of this proteinase is modulated by NS4A, a 54-amino-acid polyprotein cleavage product essential for processing at the NS3/4A, NS4A/4B, and NS4B/5A sites and enhancing cleavage efficiency between NS5A and NS5B. Using the vaccinia virus-T7 hybrid system to express hepatitis C virus polypeptides in BHK-21 cells, we studied the role of NS4A in proteinase activation. We found that the NS3 proteinase a…