Search results for "Sorting"
showing 10 items of 187 documents
Efficient selection of silenced primary cells by flow cytometry
2007
Background: RNA interference has emerged as a new and potent tool to knockdown the expression of target genes and to investigate their functions. For short time experiments with mammalian cell lines, RNA interference is typically induced by transfecting small interfering RNAs (siRNAs). Primary cells constitute important experimental systems in many studies because of their similarity to their in vivo counterparts; however, transfection of these cells has been found to be difficult. As a consequence, RNA interference of primary cells may result in mixed phenotypes because of the simultaneous presence in the same preparation of transfected and nontransfected cells. This may be particularly in…
Suffix array and Lyndon factorization of a text
2014
Abstract The main goal of this paper is to highlight the relationship between the suffix array of a text and its Lyndon factorization. It is proved in [15] that one can obtain the Lyndon factorization of a text from its suffix array. Conversely, here we show a new method for constructing the suffix array of a text that takes advantage of its Lyndon factorization. The surprising consequence of our results is that, in order to construct the suffix array, the local suffixes inside each Lyndon factor can be separately processed, allowing different implementative scenarios, such as online, external and internal memory, or parallel implementations. Based on our results, the algorithm that we prop…
Self-assembly of metallosupramolecular rhombi from chiral concave 9,9'-spirobifluorene-derived bis(pyridine) ligands.
2014
Two new 9,9’-spirobifluorene-based bis(4-pyridines) were synthesised in enantiopure and one also in racemic form. These ligands act as concave templates and form metallosupramolecular [(dppp)2M2L2] rhombi with cis-protected [(dppp)Pd]2+ and [(dppp)Pt]2+ ions. The self-assembly process of the racemic ligand preferably occurs in a narcissistic self-recognising manner. Hence, a mixture of all three possible stereoisomers [(dppp)2M2{(R)-L}2](OTf)4, [(dppp)2M2{(S)-L}2](OTf)4, and [(dppp)2M2{(R)-L}{(S)-L}](OTf)4 was obtained in an approximate 1.5:1.5:1 ratio which corresponds to an amplification of the homochiral assemblies by a factor of approximately three as evidenced by NMR spectroscopy and m…
Solvent-free ball-milling subcomponent synthesis of metallosupramolecular complexes.
2015
Subcomponent self-assembly from components A, B, C, D, and Fe(2+) under solvent-free conditions by self-sorting leads to the construction of three structurally different metallosupramolecular iron(II) complexes. Under carefully selected ball-milling conditions, tetranuclear [Fe4 (AD2 )6 ](4-) 22-component cage 1, dinuclear [Fe2 (BD2 )3 ](2-) 11-component helicate 2, and 5-component mononuclear [Fe(CD3 )](2+) complex 3 were prepared simultaneously in a one-pot reaction from 38 components. Through subcomponent substitution reaction by adding subcomponent B, the [Fe4 (AD2 )6 ](4-) cage converts quantitatively to the [Fe2 (BD2 )3 ](2-) helicate, which, in turn, upon addition of subcomponent C, …
Dificultades para Codificar, Relacionar y Categorizar Problemas Verbales Algebraicos: dos estudios con estudiantes de secundaria y profesores en form…
2014
En resolución de problemas verbales por transferencia, la activación de problemas ya conocidos que sirvan de guía, depende de las analogías percibidas entre éstos y el problema a resolver. Se desarrollan dos estudios relacionados para analizar en qué características se basan los estudiantes para codificar problemas y detectar sus analogías, en tareas de categorización (sorting). Se utilizaron técnicas cuantitativas y cualitativas combinadas. Primero se analizó cómo los estudiantes de secundaria son influidos por diferentes variables características de problemas de ciencias. Una gran proporción de sujetos no fue capaz de percibir las analogías y diferencias adecuadas entre problemas. El segu…
Protein Kinase C-dependent Ubiquitination and Clathrin-mediated Endocytosis of the Cationic Amino Acid Transporter CAT-1*
2011
Cationic amino acid transporter 1 (CAT-1) is responsible for the bulk of the uptake of cationic amino acids in most mammalian cells. Activation of protein kinase C (PKC) leads to down-regulation of the cell surface CAT-1. To examine the mechanisms of PKC-induced down-regulation of CAT-1, a functional mutant of CAT-1 (CAT-1-HA-GFP) was generated in which a hemagglutinin antigen (HA) epitope tag was introduced into the second extracellular loop and GFP was attached to the carboxyl terminus. CAT-1-HA-GFP was stably expressed in porcine aorthic endothelial and human epithelial kidney (HEK) 293 cells. Using the HA antibody internalization assay we have demonstrated that PKC-dependent endocytosis…
New Representations for Multidimensional Functions Based on Kolmogorov Superposition Theorem. Applications on Image Processing
2012
Mastering the sorting of the data in signal (nD) can lead to multiple applications like new compression, transmission, watermarking, encryption methods and even new processing methods for image. Some authors in the past decades have proposed to use these approaches for image compression, indexing, median filtering, mathematical morphology, encryption. A mathematical rigorous way for doing such a study has been introduced by Andrei Nikolaievitch Kolmogorov (1903-1987) in 1957 and recent results have provided constructive ways and practical algorithms for implementing the Kolmogorov theorem. We propose in this paper to present those algorithms and some preliminary results obtained by our team…
Sorting and recycling of waste in Latvia in the context of circular economy goals
2020
Nepаreizа sаdzīves аtkritumu iznīcināšаnа ir rаdījusi lielu kаitējumu videi, kаs ir viens no gаlvenаjiem cēloņiem аugstаjаm piesārņojumа līmenim Eiropā. Līdz аr to Eiropаs Komisijа ir izstrādājusi аprites ekonomikаs plānu, kurā ir detаlizēti norādītаs аtkritumu аpsаimniekošаnаs vаdlīnijаs pārejаi uz аprites ekonomiku. Mаģistrа dаrbа mērķis ir novērtēt аtkritumu аpsаimniekošаnаs jomаs аttīstībаs progresu un tendences nākotnē un noteikt, vаi Lаtvijа spēs sаsniegt Eiropаs Komisijаs izvirzītos аprites ekonomikаs pаketes аtkritumu pārstrādes un аpglаbāšаnаs mērķus. Аr iedzīvotāju аptаuju аnаlīzi un lаikа rindu prognozēšаnu tikа konstаtēts, kа Lаtvijā neizdosies sаsniegt аprites ekonomikаs izvirz…
One-sensor electronic olfactometer for rapid sorting of fresh fruit juices
2001
Abstract A low-cost electronic olfactometer was developed, based on virtual sensors array (an array of signals from a single metal oxide sensor) and on our proprietary semi-automated sampling system. This concept enables us to drastically improve the sensitivity and selectivity of the entire olfactometer to make it suited to food products analysis. Each step of the prototype was checked separately in earlier work, and finally the system performance was evaluated first on pure chemicals, and then at-line for grape juice during grape-harvesting in Champagne. It is shown that over a 3-week vintage period, the drift of the unique-sensor was quite low (absolute baseline value: avg., 2152; std., …
Signal sequences modulate the immunogenic performance of human hepatitis C virus E2 gene
2005
Abstract Envelope protein E2 of human hepatitis C virus (HCV) is an attractive component of a prototype HCV vaccine. Delivered by DNA immunogens, E2 evokes specific immune response of Th1-type, failing to induce either considerable antibody production, or T-helper cell proliferation. We aimed at modulating the immunogenic performance of E2 gene by changing the mode of protein expression in eukaryotic cells. Plasmids were constructed encoding full-length E2 and nonstructural protein 1 (p7) fused to either 13 or 38 C-terminal amino acids (aa) of HCV E1 that contain second hydrophobic segment of E1 stop-transfer signal, or a complete E1 stop-transfer signal with duplicated second hydrophobic s…