Search results for "Sperm"
showing 10 items of 658 documents
The role of Doppler ultrasonography in determining the proper surgical approach to the management of varicocele in children and adolescents
2006
In a study from Italy, colour-Doppler ultrasonography was a reliable diagnostic tool in the preoperative assessment of patients with varicocele. The authors also found that it helped to distinguish those who could be treated laparoscopically from those who should be treated by microsurgical subinguinal ligature. OBJECTIVE To investigate whether colour Doppler ultrasonography (CDUS) is a reliable diagnostic tool for selecting patients with varicocele to undergo either laparoscopy or open microsurgical subinguinal ligation. PATIENTS AND METHODS In a 3-year period, 42 boys affected by left varicocele were evaluated before surgery by inguinal and scrotal CDUS. Using this method it was possible …
Safety and efficacy of intensity-modulated radiotherapy in the management of spermatic cord sarcoma.
2017
International audience; Purpose. - Spermatic cord sarcoma is a rare disease, which management remains controversial due to the lack of guidelines. The standard therapeutic approach is surgical: wide soft-tissue resection with radical inguinal orchidectomy, The diagnosis is made during the analysis of the specimen. The high rate of local recurrence indicates adjuvant radiotherapy of the tumor bed. The aim of this series is to determine the efficacy and safety of postoperative intensity-modulated radiotherapy for spermatic cord sarcomas. Patients and methods. - Our series included five consecutive cases of spermatic cord sarcoma treated between 2011 and 2014. The indications for radiotherapy …
Differential expression of estrogen receptors (ER?/ER?) in testis of mature and immature pigs
2004
High affinity estrogen receptors (ERs) mediate estrogen action in male reproductive tissues. The objective of the present study was the immunolocalization of estrogen receptor alpha and estrogen receptor beta in immature and mature testes of pig, a species in which the role of estrogens on gonadal function is scarcely known. Testes from 3 and 18 month-old pigs were investigated. Immunohistochemistry was performed on paraffin embedded-tissues using both mouse anti-human monoclonal IgG ERalpha and IgG ERbeta 1 isoform. Western blot analysis demonstrated antibody specificity. ERalpha staining was not observed in immature testes, but it was detected in spermatogonia, spermatocytes and in the mo…
Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination
2021
The aim of the study was to compare the morphometric features of sperm head sizeand shape from the Pietrain line and the Duroc×Pietrain boar crossbred terminal lines, and toevaluate their relationship with reproductive success after artificial insemination of sows producedfrom crossbreeding the York, Landrace and Pietrain breeds. Semen samples were collected from 11sexually mature boars. Only ejaculates with greater than 70% motility rate and <15% of abnormalsperm were used for artificial inseminations (AI) and included in the study. Samples were analyzedusing an ISAS®v1 computer-assisted sperm analysis system for eight morphometric parameters ofhead shape and size (CASA-Morph). Sub-populat…
Relationship between Fertility Traits and Kinematics in Clusters of Boar Ejaculates
2021
The aim was to determine the relationship between kinematic parameters of boar spermatozoa and fertility rates of sow, as well as to assess the effect of sperm clusters on the fertility capacity of the ejaculate. Semen samples were collected from 11 sexually mature boars. Samples were analyzed by an ISAS®v1 CASA-Mot system for eight kinematic parameters. Ejaculate clusters were characterized using multivariate procedures, such as principal factors (PFs) analysis and clustering methods (the k-means model). Four different ejaculate clusters were identified from two kinematic PFs which involved linear trajectory and velocity. There were differences (p 0.05) between dam lines and ejaculate clus…
Aminopropyltransferases involved in polyamine biosynthesis localize preferentially in the nucleus of plant cells
2012
Plant aminopropyltransferases consist of a group of enzymes that transfer aminopropyl groups derived from decarboxylated S-adenosyl-methionine (dcAdoMet or dcSAM) to propylamine acceptors to produce polyamines, ubiquitous metabolites with positive charge at physiological pH. Spermidine synthase (SPDS) uses putrescine as amino acceptor to form spermidine, whereas spermine synthase (SPMS) and thermospermine synthase (TSPMS) use spermidine as acceptor to synthesize the isomers spermine and thermospermine respectively. In previous work it was shown that both SPDS1 and SPDS2 can physically interact with SPMS although no data concerning the subcellular localization was reported. Here we study the…
Spectral and microscopic study of self-assembly of novel cationic spermine amides of betulinic acid
2016
Supramolecular characteristics of two spermine amides of betulinic acid (1 and 2) were studied by measuring and evaluating their UV-VIS-NIR spectra in aqueous acetonitrile and DOSY-NMR spectra in tetradeuteromethanol, accompanied by atomic force microscopy (AFM) images, scanning electron microscopy (SEM) micrographs, and transmission electron microscopy (TEM) micrographs. Fibrous supramolecular self-assembly of 1 and 2 was observed by AFM images, as well as by the SEM and TEM micrographs. Bathochromic shifts of the absorbance maximum at 870nm to 1015-970nm in the UV-VIS-NIR spectra were observed with increasing water content in the acetonitrile/water systems, indicating formation of fibrous…
Treatment with Calcium Ionophore Improves The Results in Patients with Previous Unsuccessful Attempts at The Fertilization: A Cohort Study
2021
Background: The objective of this study is to evaluate artificial oocyte activation (AOA) with calcium ionophore (CaI) in a subsequent attempt at fertilisation in patients after extremely low or failed fertilisation. We assessed improvements in fertilisation, implantation and pregnancy rates as well as cancellation rates in these patients. Finally, was evaluated the result testing in addition to delivery rate and obstetric outcomes in children born after AOA. Materials and Methods: This was a retrospective observational study conducted in an IVF laboratory of an IVI clinic (IVIRMA Valencia, Spain). One group (509 mature oocytes from 66 patients) received a first intracytoplasmic sperm injec…
Influencia de los distintos tipos y parámetros del ejercicio físico sobre la calidad seminal: una revisión sistemática de la literatura
2019
Es de sobra conocida la importancia positiva de la actividad fisica correctamente prescrita en terminos de intensidad y volumen sobre diferentes alteraciones metabolicas, osteomusculares, cognitivas, etc. La infertilidad es una patologia cuya epidemiologia esta al alza por los cambios en los habitos de nuestra vida cotidiana, como la dieta inadecuada o la radiacion de las nuevas generaciones de aparatos electricos. No existen demasiadas evidencias divulgativas sobre sus causas y efectos, asi como tampoco del papel del ejercicio en la reversion de sus sintomas. Con la finalidad de obtener y sintetizar la informacion mas relevante sobre la influencia de los diferentes tipos y parametros del e…
No evidence for prezygotic postcopulatory avoidance of kin despite high inbreeding depression.
2018
11 pages; International audience; Offspring resulting from mating among close relatives can suffer from impaired fitness through the expression of recessive alleles with deleterious effects. Postcopulatory sperm selection (a prezygotic mechanism of cryptic female choice) has been suggested to be an effective way to avoid inbreeding. To investigate whether postcopulatory female choice allows the avoidance of fertilization by close kin, we performed artificial inseminations in a promiscuous bird, the houbara bustard (Chlamydotis undulata undulata). Females were inseminated with a mix of sperm from triads of males, each constituted of a male genetically unrelated to the female, a first cousin …