Search results for "Spinal"

showing 10 items of 906 documents

Chronic otitis caused by heterotopic brain tissue in pterygopalatine fossa

2007

Summary Heterotopic brain tissue is a rare is congenital anomaly, it may present at any age but it is frequently in infancy. This anomaly can occur most frequently in nasal region, although rests elsewhere in the digestive tract, in facial tissue or in lungs have been reported. Heterotopic brain tissue has been defined as a mass composed of mature brain tissue, outside the cranial cavity or spinal canal. We present a 9 years old girl with history of left chronic otitis and nasal obstruction caused by heterotopic brain tissue in pterygopalatine fossa.

Heterotopic brain tissuebusiness.industryChronic otitisChronic otitisBrain tissueAnatomymedicine.diseaseConductive hearing lossConductive hearing lossParapharyngeal spacemedicine.anatomical_structureOtorhinolaryngologyNasal regionPediatrics Perinatology and Child HealthParapharyngeal spacemedicineCranial cavitySpinal canalbusinessGlue earPterygopalatine fossaInternational Journal of Pediatric Otorhinolaryngology Extra
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Carrier screening for spinal muscular atrophy in Italian population

2014

Spinal muscular atrophy (SMA) is an autosomal-recessive neuromuscular disorder characterized by motor neuron degeneration in the anterior horn of the spinal cord and brain stem, resulting in progressive muscle weakness and atrophy. The responsible survival motor neuron gene (SMN1; HGNC: 11117) is localized in 5q11.2-13.3. Screening for carriers of SMA is necessary for effective clinical/prenatal diagnosis and genetic counselling. In this study, the copy number of SMN1 gene was determined from a southern Italian population to estimate carrier frequency. This is the first report addressing the estimation of SMA carrier frequency in an Italian population. Our results show that the SMA carrier …

HeterozygoteGenetic counselingGene DosagePhysiologycarrier screeningPrenatal diagnosisSMN1BiologyCarrier testingMuscular Atrophy SpinalAtrophyGene FrequencySettore BIO/13 - Biologia ApplicataPrevalenceGeneticsmedicineHumansGenetic Testingspinal muscular atrophysurvival motor neuron gene (SMN1); spinal muscular atrophy; carrier screening; MLPAExonsSpinal muscular atrophyMotor neuronSMA*medicine.diseaseSurvival of Motor Neuron 1 ProteinMLPAmedicine.anatomical_structureItalysurvival motor neuron gene (SMN1)Journal of Genetics
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High Rate of Studies with Level 1 and 2 Evidence among the 100 Most Cited Articles in Lumbar Spinal Stenosis.

2021

Abstract Background To date, no study has used bibliometric analysis to review the most influential articles in lumbar spinal stenosis. The objective of this study was to identify and analyze the characteristics and the level of evidence of the 100 most cited articles on lumbar spinal stenosis Methods The Thomson Reuters Web of Science was accessed to find the 100 most cited articles on lumbar spinal stenosis. For each article, we recorded the number and density of citations, authors, country, journals and years, department, level of evidence, type of study, and if it was part of any multicenter studies. Results Until January 2017, the 100 most cited articles accumulated 11,136 citations (a…

High ratemedicine.medical_specialtyBibliometric analysisbusiness.industryCitation indexMEDLINELumbar spinal stenosisEvidence-based medicinemedicine.diseaseSpine03 medical and health sciences0302 clinical medicineSpinal Stenosis030202 anesthesiologyBibliometricsOrthopedic surgeryPhysical therapymedicineRelated researchHumansSurgeryNeurology (clinical)business030217 neurology & neurosurgeryJournal of neurological surgery. Part A, Central European neurosurgery
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Skoliose und Hüftbeugekontraktur bei Duchennescher Muskel-dystrophie

2000

UNLABELLED Spinal deformity is common in muscular dystrophy and usually occurs after loss of walking ability. Unlike in idiopathic and other scoliosis forms, there seems to be no side preference of the convexity. Aim of the study was to analyse, if there is any relation between incidence and extent of walking ability, lower limb contractures and development of scoliosis. METHODS In a retrospective study, 45 patients with Duchenne muscular dystrophy who underwent surgery were analysed, concerning walking ability, contractures of lower extremities and scoliosis. RESULTS 1: No scoliosis was observed in ambulatory patients. 2: 96% of the wheelchair bound patients suffered from scoliosis. 3: 96%…

Hip surgerymedicine.medical_specialtybusiness.industryDuchenne muscular dystrophymedicine.medical_treatmentScoliosismedicine.diseaseSurgerybody regionsSpinal fusionHip ContracturemedicineOrthopedics and Sports MedicineSurgeryContracturemedicine.symptomMuscular dystrophybusinessMuscle contractureZeitschrift für Orthopädie und ihre Grenzgebiete
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Effects of Anandamide and Noxious Heat on Intracellular Calcium Concentration in Nociceptive DRG Neurons of Rats

2007

As an endogenous agonist at the cannabinoid receptor CB1 and the capsaicin-receptor TRPV1, anandamide may exert both anti- and pronociceptive actions. Therefore we studied the effects of anandamide and other activators of both receptors on changes in free cytosolic calcium ([Ca2+]i) in acutely dissociated small dorsal root ganglion neurons (diameter: ≤30 μm). Anandamide (10 μM) increased [Ca2+]iin 76% of the neurons. The EC50was 7.41 μM, the Hill slope was 2.15 ± 0.43 (mean ± SE). This increase was blocked by the competitive TRPV1-antagonist capsazepine (10 μM) and in Ca2+-free extracellular solution. Neither exclusion of voltage-gated sodium channels nor additional blockade of voltage-gate…

Hot TemperatureCannabinoid receptorPolyunsaturated AlkamidesPhysiologyTRPV1TRPV Cation ChannelsArachidonic AcidsPharmacologyCalcium in biologyRats Sprague-Dawleychemistry.chemical_compoundGanglia SpinalPhysical StimulationAnimalsDrug InteractionsDronabinolEgtazic AcidChelating AgentsNeuronsCalcium metabolismAnalysis of VarianceDose-Response Relationship DrugChemistryGeneral NeuroscienceExtracellular FluidAnandamideCalcium Channel BlockersEndocannabinoid systemRatsNociceptionCalciumCapsaicinEndogenous agonistEndocannabinoidsJournal of Neurophysiology
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Coexpression of heat-evoked and capsaicin-evoked inward currents in acutely dissociated rat dorsal root ganglion neurons.

1997

Noxious heat is able to activate heat-sensitive nociceptors in the skin very rapidly, but little is known about the mechanisms by which heat is transduced. We used the whole-cell patch-clamp technique to study the effects of noxious heat and capsaicin on freshly dissociated rat dorsal root ganglion neurons in vitro. Using temperatures between 41 degrees C and 53 degrees C, 8 of 19 small neurons (phior = 30 microm) exhibited a heat-evoked inward current. All heat-sensitive neurons tested were also capsaicin-sensitive. Moreover, the heat response tended to be enhanced after capsaicin (360 +/- 150 pA versus 125 +/- 45 pA, P0.1, n = 7). Two of five heat-insensitive neurons were excited by capsa…

Hot TemperaturePatch-Clamp TechniquesCentral nervous systemRats Sprague-Dawley03 medical and health scienceschemistry.chemical_compound0302 clinical medicineDorsal root ganglionGanglia SpinalmedicineAnimalsPatch clamp030304 developmental biologyCell SizeNeurons0303 health sciencesGeneral NeuroscienceReproducibility of ResultsSpinal cordSensory neuronRatsmedicine.anatomical_structureNociceptionnervous systemchemistryCapsaicinNociceptorBiophysicsCapsaicinNeuroscience030217 neurology & neurosurgeryNeuroscience letters
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DNA Methylation and Non-Coding RNAs during Tissue-Injury Associated Pain.

2022

While about half of the population experience persistent pain associated with tissue damages during their lifetime, current symptom-based approaches often fail to reduce such pain to a satisfactory level. To provide better patient care, mechanism-based analgesic approaches must be developed, which necessitates a comprehensive understanding of the nociceptive mechanism leading to tissue injury-associated persistent pain. Epigenetic events leading the altered transcription in the nervous system are pivotal in the maintenance of pain in tissue injury. However, the mechanisms through which those events contribute to the persistence of pain are not fully understood. This review provides a summar…

INFLAMMATORY PAINRNA UntranslatedChemistry MultidisciplinaryAdaptation BiologicalReviewUP-REGULATIONEpigenesis GeneticCpG islandsTranscripció genèticalncRNANeurociènciesnociceptionBiology (General)SpectroscopyGENE-EXPRESSIONGeneral MedicineComputer Science ApplicationsChemistryPhysical SciencesDisease SusceptibilityChronic PainLife Sciences & BiomedicineepigeneticALLEVIATES NEUROPATHIC PAINBiochemistry & Molecular Biologydorsal root ganglionQH301-705.50699 Other Biological SciencesCatalysisCONTRIBUTESInorganic ChemistryDiagnosis DifferentialCENTRAL SENSITIZATION0399 Other Chemical SciencesHumansPhysical and Theoretical ChemistryQD1-999Molecular Biologyspinal dorsal hornmiRNACHRONIC CONSTRICTION INJURYneuropathic pain0604 GeneticsScience & TechnologyChemical PhysicsNERVE INJURYMICRORNAGene Expression ProfilingOrganic ChemistryDNA MethylationCPG-BINDING PROTEIN-2gene transcriptionGene Expression RegulationsiRNARNAWounds and InjuriesBiomarkersInternational journal of molecular sciences
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Post-translational modifications in the survival motor neuron protein

2004

Spinal muscular atrophy (SMA) is an autosomal recessive disease characterized by a progressive loss of the spinal motoneurons. The SMA-determining gene has been termed survival motor neuron (SMN) and is deleted or mutated in over 98% of patients. The encoded gene product is a protein expressed as different isoforms. In particular, we showed that the rat SMN cDNA produces two isoforms with Mr of 32 and 35 kDa, both localized in nuclear coiled bodies, but the 32 kDa form is also cytoplasmic, whereas the 35 kDa form is also microsomal. To determine the molecular relationship between these two isoforms and potential post-translational modifications, we performed transfection experiments with a …

INVOLVEMENTFORMSPRODUCTBiochemistryMiceChlorocebus aethiopsProtein IsoformsPhosphorylationCyclic AMP Response Element-Binding ProteinSMN PROTEINCells CulturedMotor NeuronsSPINAL MUSCULAR-ATROPHYRNA-Binding ProteinsSMN Complex Proteins3T3 CellsTransfectionmedicine.anatomical_structureSpinal CordCOS CellsSUBCELLULAR-LOCALIZATIONEXPRESSIONGene isoformRecombinant Fusion ProteinsBiophysicsNerve Tissue ProteinsBiologyMuscular Atrophy SpinalGene productSMN Complex ProteinsComplementary DNAmedicineAnimalsHumansMolecular BiologyCell BiologySpinal muscular atrophyMotor neuronmedicine.diseaseSurvival of Motor Neuron 1 ProteinMolecular biologyRatsnervous system diseasesMolecular WeightSEVERITYnervous systemBODIESProtein Processing Post-TranslationalDETERMINING GENEImmunostainingBiochemical and Biophysical Research Communications
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Clinical Use of κ Free Light Chains Index as a Screening Test for Multiple Sclerosis.

2020

Abstract Objective To assess the usefulness of the κ free light chain index (κFLCi) as a screening test to identify patients with suspected MS. Methods The study included 56 patients with a request to test for oligoclonal bands (OCBs). OCBs were detected by isoelectric focusing, followed by immunofixation. Cerebrospinal fluid (CSF) and serum κFLC were measured by a turbidimetric assay. Also, the κFLC index (κFLCi) was calculated. Results CSF κFLC levels and κFLCi were significantly higher in patients with multiple sclerosis (MS) than in patients with other neurological diseases (NDs; P < .001 and P < .001, respectively). At the cutoff value of 2.9, the κFLCi detected MS with s…

ImmunofixationAdultMale030213 general clinical medicinemedicine.medical_specialtyMultiple SclerosisScreening testClinical BiochemistryκFLCCSFfree light chainImmunoglobulin light chainGastroenterologySensitivity and Specificity03 medical and health sciences0302 clinical medicineCerebrospinal fluidInternal medicinemedicineHumansMass ScreeningAgedOCBbiologybusiness.industryIsoelectric focusingMultiple sclerosisBiochemistry (medical)Middle AgedκFLC indexmedicine.diseaseFree Light Chainmultiple sclerosibiology.proteinbiomarkerBiomarker (medicine)FemaleImmunoglobulin Light Chainsbusiness030217 neurology & neurosurgeryBiomarkersLaboratory medicine
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Increased cerebrospinal fluid albumin and immunoglobulin A fractions forecast cortical atrophy and longitudinal functional deterioration in relapsing…

2017

Background: Currently, no unequivocal predictors of disease evolution exist in patients with multiple sclerosis (MS). Cortical atrophy measurements are, however, closely associated with cumulative disability. Objective: Here, we aim to forecast longitudinal magnetic resonance imaging (MRI)-driven cortical atrophy and clinical disability from cerebrospinal fluid (CSF) markers. Methods: We analyzed CSF fractions of albumin and immunoglobulins (Ig) A, G, and M and their CSF to serum quotients. Results: Widespread atrophy was highly associated with increased baseline CSF concentrations and quotients of albumin and IgA. Patients with increased CSFIgA and CSFIgM showed higher functional disabilit…

Immunoglobulin AAdultMalePathologymedicine.medical_specialty03 medical and health sciencesYoung Adult0302 clinical medicineCerebrospinal fluidMultiple Sclerosis Relapsing-RemittingAlbuminsmedicineHumansIn patient030212 general & internal medicineLongitudinal StudiesCortical atrophyCerebral Cortexbiologybusiness.industryMultiple sclerosisAlbuminmedicine.diseasePrognosisImmunoglobulin ADisease evolutionNeurologyRelapsing remittingbiology.proteinFemaleNeurology (clinical)Atrophybusiness030217 neurology & neurosurgeryBiomarkersMultiple sclerosis (Houndmills, Basingstoke, England)
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