Search results for "Spiramycin"

Determination of macrolide and lincosamide antibiotics by pressurised liquid extraction and liquid chromatography-tandem mass spectrometry in meat an…

2010

Abstract A total of 155 samples, including meat and milk, were analyzed using a method developed for determining five macrolide antibiotics: tilmicosin (TILM), tylosin (TS), spiramycin (SPM), erythromycin (ERY), and tulathromycin (TULA) and two lincosamide antibiotics: pirlimycin (PIRL) and lincomycin (LM). The method was performed by pressurised liquid extraction (PLE) coupled to LC–MS/MS using electrospray ionization in positive ion mode. 2.5 mL of milk or 2.5 g of meat were dispersed in ethylenediaminetetraacetic acid sodium salt 2-hydrate (EDTA) and sea sand. The PLE conditions as: solvent, temperature, pressure, static time, and cell size were optimized. The following analytical parame…

Physiology and genetics of antibiotic production and resistance.

1993

Actinomycetes have the genetic capability to synthesize many different biologically active secondary metabolites and of these compounds, antibiotics predominate in therapeutic and commercial importance. Intensive research often centres on the use of molecular techniques to investigate the physiology and genetics of antibiotic biosynthesis with a view to improving production. The isolation of clones of Streptomyces hygroscopicus, the producer of geldanamycin, which synthesizes geldanamycin in S. lividans, is reported. Molecular approaches using genes for elongation factors (tuf) were used in attempts to increase the fermentation yield of kirromycin, whilst probes for aphD and sph, genes for …

INTESTINAL INFECTION BY CRYPTOSPORIDIUM IN THREE CHILDREN WITH ABDOMINAL PAIN AND CHRONIC DIARRHOEA

1985

Children infection by Cryptosporidium sp. have been communicated since 1976 usually associated to acute diarrohoeal disease. This protozoa has been isolated recently in SIDA patients with serious illness. Three new cases, the first of spanish pediatric literature, are described in this paper. Children were males; two of them twins. Age ranged 24 to 27 m. All lived in Valencia, and went to infant's school with high stand of living and domestic animal relationship. The most frequent symptom was well located colic abdominal pain with day predominance and 2 months of evolution in the twins. The third case began with abdominal pain and discomfort. Three months before had been treated with Metron…

Genetic manipulation of spiramycin-producing strain Streptomyces ambofaciens ATCC 23877 by φC31 Att/Int system-based vectors results in a significant…

The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and many other biologically active secondary metabolites. The genetic manipulation of Streptomycetes is often labor and time intensive due to their large genome and complex development. Much progress has been made to develop gene transfer methods useful to construct antibiotic-producing strains with improved properties. The φC31 Att/Int system is an integration system that has been widely used to produce stable recombinants in Actinomycetes and its integration site is an attB site located in a pirin-like gene (pirA) of the bacterial chromosome. In this study we demonstrate that the integration of φC31 DNA in…