Search results for "Spot"
showing 10 items of 371 documents
Friction Stir Spot Welding of AA6082-T6: influence of the most relevant process parameters and comparison with classic mechanical fastening techniques
2007
The results of an experimental study on friction stir spot welding (FSSW) of AA6082-T6 are reported. In particular, process mechanics is highlighted and joint strength is considered in relation to varying the most relevant process parameters. Furthermore, the results obtained are compared with those derived from the application of traditional mechanical fastening techniques such as clinching and riveting. In this way the effectiveness of FSSW is highlighted.
On the Presence of Pipistrelle Bats (PipistrellusandHypsugo; Chiroptera: Vespertilionidae) in Sardinia
2011
Islands are biodiversity hotspots, often containing numerous endemic species. This makes them also hotspots for conservation. Within the Mediterranean region, Sardinia is known for its comparatively high degree of endemism, including cryptic diversity. In this paper we aim to elucidate the variability of pipistrelles (Pipistrellus and Hypsugo) on Sardinia. More specifically, we ask which species occur on Sardinia and we describe the geographic affiliations of these evolutionary lineages. We sequenced ca. 560 bp of the 16S rRNA gene from 36 pipistrelle specimens representing 17 localities from all major parts of Sardinia. For comparison we added samples from the entire Mediterranean region a…
A novel NF1 mutation in a pediatric patient with renal artery aneurysm
2022
Abstract Background Neurofibromatosis type 1 (NF1) is a neurocutaneous syndrome, due to heterozygous pathogenic variants in NF1 gene. The main clinical manifestations are multiple café au lait spots, axillary and inguinal freckling, cutaneous and plexiform neurofibromas, optic glioma, Lisch nodules and osseous lesions, such as sphenoid and tibial dysplasia. Vasculopathy is another feature of NF1; it consists of stenosis, aneurysms, and arteriovenous malformations, frequently involving renal arteries. Case presentation We report on a 9-year-old girl with a novel mutation in NF1 gene and renal artery aneurysm, treated by coil embolization and complicated with hypertension. Conclusion Vasculop…
Application of TLC and GC-MS to the detection of capsaicin from hot peppers(Capsicum annuum)
2004
Capsaicin was extracted from red hot pepper (Capsicum annuum) fruits and from commercial red hot pepper powder and separated on silica gel 60 plates with concentration zone (Merck); toluene-acetone-chloroform, 45 + 30 + 25 ( v/v ), was used as mobile phase. Detection was performed by exposure to iodine vapor. The spots assumed to arise from capsaicin were removed from the plate and the components were extracted with chloroform. The extracts were analyzed by gas chromatography combined with mass spectrometry.
Analysis of nucleophosmin–anaplastic lymphoma kinase (NPM‐ALK)‐reactive CD8+ T cell responses in children with NPM‐ALK+ anaplastic large cell lymphoma
2016
Summary Cellular immune responses against the oncoantigen anaplastic lymphoma kinase (ALK) in patients with ALK-positive anaplastic large cell lymphoma (ALCL) have been detected using peptide-based approaches in individuals preselected for human leucocyte antigen (HLA)-A*02:01. In this study, we aimed to evaluate nucleophosmin (NPM)-ALK-specific CD8+ T cell responses in ALCL patients ensuring endogenous peptide processing of ALK antigens and avoiding HLA preselection. We also examined the HLA class I restriction of ALK-specific CD8+ T cells. Autologous dendritic cells (DCs) transfected with in-vitro-transcribed RNA (IVT-RNA) encoding NPM–ALK were used as antigen-presenting cells for T cell …
Reproductive site selection: evidence of an oviposition cue in a highly adaptive dipteran, Drosophila suzukii (Diptera: Drosophilidae)
2020
Abstract Drosophila suzukii (Matsumura) is a vinegar fly species that originates from Eastern Asia and has spread throughout Europe and the Americas since its initial detection in United States in 2008. Its relatively large, sclerotized, and serrated ovipositor enables the ability to penetrate ripening fruits, providing a protected environment for its egg and larval stages. Because the mechanism of oviposition site selection of D. suzukii is a matter of hypothesis, the aim of the present study was to elucidate behavioral and chemical aspects of short-range ovipositional site selection within the context of D. suzukii reproductive biology. The preference of D. suzukii to lay eggs on artifici…
A New Statistical Reconstruction Method for the Computed Tomography Using an X-Ray Tube with Flying Focal Spot
2021
Abstract This paper presents a new image reconstruction method for spiral cone- beam tomography scanners in which an X-ray tube with a flying focal spot is used. The method is based on principles related to the statistical model-based iterative reconstruction (MBIR) methodology. The proposed approach is a continuous-to-continuous data model approach, and the forward model is formulated as a shift-invariant system. This allows for avoiding a nutating reconstruction-based approach, e.g. the advanced single slice rebinning methodology (ASSR) that is usually applied in computed tomography (CT) scanners with X-ray tubes with a flying focal spot. In turn, the proposed approach allows for signific…
Hot spots and gluon field fluctuations as causes of eccentricity in small systems
2021
We calculate eccentricities in high energy proton-nucleus collisions, by calculating correlation functions of the energy density field of the Glasma immediately after the collision event at proper time tau = 0. We separately consider the effects of color charge and geometrical hot spot fluctuations, analytically performing the averages over both in a dilute-dense limit. We show that geometric fluctuations of hot spots inside the proton are the dominant source of eccentricity whereas color charge fluctuations only give a negligible correction. The size and number of hot spots are the most important parameters characterizing the eccentricities.
In Vitro Stimulation and Expansion of Human Tumour-Reactive CD8+ Cytotoxic T Lymphocytes by Anti-CD3/CD28/CD137 Magnetic Beads
2011
Adoptive immunotherapy with tumour-reactive CD8(+) cytotoxic T lymphocytes (CTLs) requires efficient in vitro approaches allowing the expansion of CTLs to large numbers prior infusion. Here, we investigated the antigen-independent activation and the expansion of human T cells in peripheral blood mononuclear cells (PBMCs) and in tumour-reactive CTLs using Dynabeads coated with monoclonal antibodies to CD3 and to the costimulatory molecules CD28 and CD137 (4-1BB). T cells in PBMCs showed an increased expansion rate of 15- to 17-fold during a 2-week culture period using antibody-conjugated beads with interleukin-2 (IL-2) added versus IL-2 alone. No significant difference between CD3/CD28 beads…
Trust, mistrust and distrust as blind spots of Social Licence to Operate: illustration via three forerunner countries in nuclear waste management
2021
The notion of social licence to operate (SLO) has become a widely applied concept for companies in mining and resource extraction industries to manage their social and community relations, in the face of local criticism and opposition. SLO literature and practice have highlighted earning the trust of the local community as a key requirement for an SLO. This article addresses three weaknesses in how the current SLO literature addresses trust. The arguments are illustrated via examples from nuclear waste management in Finland, France and Sweden–three forerunners in implementing high-level nuclear waste repository projects. Nuclear waste management constitutes a relevant case for analysis, as …