Search results for "Spot"
showing 10 items of 371 documents
Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial
2000
The ELISPOT assay is increasingly being used for the monitoring of the induction of antigen-reactive T cells in cancer vaccination trials. In order to evaluate the reliability of T cell frequency analysis with the ELISPOT assay, a comparative study was performed in four European laboratories. Six samples from healthy subjects were analyzed for the frequency of influenza-reactive CD8+ T cells in peripheral blood mononuclear cells (PBMC) by IFNgamma-ELISPOT assay. In addition, one laboratory determined cytotoxic T cell precursor (CTL) frequencies in these samples by limiting dilution chromium-release assay (LDA), and three laboratories performed a variant of the LDA, the multiple microculture…
Identification of T cell epitopes by the use of rapidly generated mRNA fragments.
2004
Abstract Although the number of defined T cell epitopes of clinically relevant antigens is constantly increasing, there is still an enormous need to identify further peptides, processed from new antigens or presented by rare HLA molecules, respectively. Here we introduce a novel two-step approach for the rapid identification of T cell epitopes. It was established in the CMV infection model. From the peripheral blood of healthy donors sharing HLA-A1 according to HLA serotyping we isolated CD8 + T lymphocytes and generated dendritic cells (DCs). DCs were electroporated with CMV pp65 mRNA and tested for recognition by autologous CD8 + T lymphocytes in IFN-γ ELISPOT assays. In all 10 CMV-seropo…
The use of clonal mRNA as an antigenic format for the detection of antigen-specific T lymphocytes in IFN-gamma ELISPOT assays.
2003
Abstract Most assay systems for the quantification of antigen-specific T-cell responses in infectious, malignant and autoimmune disease depend on the peptide antigen format and are therefore restricted to known epitopes and their presenting HLA molecules. Here we tested in ELISPOT assays the application of in vitro-transcribed clonal mRNA as an alternative antigen format covering all potential epitopes of a given antigen. As model antigens, we chose pp65 of human cytomegalovirus (HCMV) and human tyrosinase (hTyr). Antigen-presenting cells (APC) were K562 cells stably transfected with single HLA class I alleles and autologous dendritic cells (DC). As effectors, we applied in vitro-generated …
Editorial: Activation, functions, and generation of immunological memory in γδ T lymphocytes: lessons from nonhuman primates
2014
T cells constitute an unconventional lymphocyte population with distinct functions complementary to those of CD4 and CD8 T cells. As such, they have both adaptive features, such as expression of the TCR, and innate-like functions reminiscent of NK cells, with whom they share extensive repertoires of activating and inhibitory receptors [1, 2]. Although most antigens recognized by murine T cells remain obscure, advances have been made in identifying ligands for human T cells. The majority of circulating human T lymphocytes expresses a TCR formed by the preferentially-paired V 9 and V 2 chains (here and thereafter, called V 9V 2 T cells). Instead of binding peptides associated with molecules b…
Distribution of graminoids in open habitats in Tajikistan and Kyrgyzstan
2022
Aims: Landscapes of Middle Asia are exposed to human influence due to long-lasting pastoral tradition, and now are largely dominated by non-forest vegetation. Graminoids perform key ecosystem functions, and constitute an important feed source for livestock. We studied the distribution patterns of graminoids cover under climatic and grazing pressure gradients in different open vegetation types. Study area: Tajikistan, Kyrgyzstan. Methods: 1,525 vegetation plots representing five open vegetation types (mires, salt marshes, tall-forb communities, pseudosteppes and steppes) were extracted from the Vegetation of Middle Asia Database. We assessed the relative cover of graminoid species in each ve…
A tooth whitening and chemical abrasive protocol for the treatment of developmental enamel defects
2018
Abstract This clinical report describes a chemical abrasive protocol and whitening techniques to quantify modifications to the color and volume produced when eliminating white spots associated with developmental defects in enamel. Chemical (oxidative-erosive) abrasive treatment (whitening + 6.6% hydrochloric acid) resolves white spots up to a depth of 0.2 mm. At greater depths, infiltration with 16% hydrochloric acid followed by composite resin infiltration is recommended.
What is a tree in the Mediterranean Basin hotspot? A critical analysis
2019
Background: Tree species represent 20% of the vascular plant species worldwide and they play a crucial role in the global functioning of the biosphere. The Mediterranean Basin is one of the 36 world biodiversity hotspots, and it is estimated that forests covered 82% of the landscape before the first human impacts, thousands of years ago. However, the spatial distribution of the Mediterranean biodiversity is still imperfectly known, and a focus on tree species constitutes a key issue for understanding forest functioning and develop conservation strategies. Methods: We provide the first comprehensive checklist of all native tree taxa (species and subspecies) present in the Mediterranean-Europ…
Expression of phase-specific haemolymph polypeptides in a laboratory strain and field catches of Schistocerca gregaria.
2003
Uvarov's theory of locust phase polymorphism implies a differential phase-specific gene expression. It was the goal of the present investigation to provide data on the molecular level for this concept. We used the technique of 2D gel electrophoresis to generate haemolymph polypeptide maps from mature locust males. Under the given conditions we identified 238 polypeptide spots by their molecular weight and isoelectrical point. Isolated and crowded males from the Mainz strain, all originating from crowded ancesters, differed in 20 spots: three were solitary-specific and 17 crowded-specific. Field catches of solitary and gregarious S. gregaria in Mauritania revealed that those males showed the…
Le corps du délit: spot publicitaire et mythe technologique
1991
Ce texte passe en revue l'imagerie publicitaire lorsqu'elle a affaire avec la technologie moderne. La limpidité des formes et les métaphores astrales suggèrent un space incontaminé proche au mythe.
Uzņēmuma vērtības vadīšanas pilnveidošana multimediju straumēšanas nozarē.
2019
Ar uzņēmuma finansiālajiem rādītājiem ir iespējams noteikt cik efektīvi uzņēmums nodarbojas ar saimniecisko darbību. Vadībai pieņemot tālākās attīstības stratēģijas ir nepieciešams ņemt tos vērā un lēmumu balstīt uz tiem. Vērtības rādītājs ir lielums, kas aptver daudzus finansiālos faktorus vienkopus. Pētījums ir veikts, lai izprastu vērtības nozīmību strauji augošajā multimediju straumēšanas nozarē, kur ir raksturīgas augstas uzņēmumu tirgus vērtības pret uzņēmumu aktīvu daudzumu un ienākumiem. Pētījumā tiek veikta uzņēmumu vērtību detalizācija, lai izprastu tiešo vērtības nozīmi un tās ietekmējošos faktorus. Pētījuma rezultātā tiek iegūtas uzņēmumu vērtības, kas tiek aprēķinātas pēc vairā…