Search results for "Stability."
showing 10 items of 3015 documents
Fifteen years’ experience treating cells with inorganic arsenic, a molecule able to induce genetic / genomic instability and epigenetic changes even …
2016
Treating V79-Cl3 cells with 10 µM sodium arsenite (SA) for 24h we observed severe alterations in spindle morphology and aneuploidy; treating rat astrocytes with SA we detected HSP70 induction and DNA damage. We assumed that SA induced in dividing cells early genetic instability. Subsequently, we in vitro stabilized those V79-Cl3 cells dividing at the end of SA-treatment and maintained for long without SA (ASO cells). In ASO cells, we observed chromosomal rearrangements, increased spontaneous mutations, genome-wide DNA hypomethylation (GWDH), similarly to exposed cells. We inferred that a short-term SA exposure has long-term effects and that GWDH enhances the genetic instability. Consequentl…
Persistent dysregulation of DNA methylation in cells with arsenic-induced genomic instability
2009
The mechanisms by which arsenic-induced genomic instability is initiated and maintained are poorly understood. In previous studies long-term progression of chromosomal instability was typified by increasing aneuploidy in Chinese hamster V79 and human keratinocyte cells treated with arsenite for a 24 hr exposure period followed by growth in arsenic-free medium for 40-120 cell generations. In the current study the role of progressive DNA methylation changes was evaluated in long-term cell cultures after brief arsenite treatments as above. We have found altered genomic methylation patterns in cells that were briefly exposed to arsenic with evidence for widespread dysregulation of CpG methylati…
Identification of molecular markers involved in cellular response to aneuploidy in normal and tumor cells
2020
Long-term exposure to submicromolar arsenite induces bypass of the spindle assembly checkpoint in mammalian cells
2008
Mitosis is regulated by checkpoints that delay mitotic progression when chromosome segregation errors occur. Inaccuracy in checkpoint processes can lead to chromosome instability both in number and structure (CIN). Arsenic is reported to induce CIN by perturbing mitotic spindles and checkpoints, however, its carcinogenic mechanisms are poorly understood. We previously studied the long-term progression of chromosomal instability in V79 cells treated acutely with arsenite (10 M, 24 hr) followed by growth in arsenic-free medium for 120 cell generations, and found time-dependent increase of aneuploid cells. Here, we treated V79-derived G12 cells with sub-lethal doses (0.1-1.0 µM) of arsenite f…
DNA DEMETHYLATION CAUSED BY 5-AZA-2’-DEOXYCYTIDINE TREATMENT AFFECTS GENOME STABILITY BY INDUCING MITOTIC ALTERATIONS AND ANEUPLOIDY IN HCT-116 TUMOR…
2014
FANCD2 promotes mitotic rescue from transcription-mediated replication stress in SETX-deficient cancer cells
2022
AbstractReplication stress (RS) is a leading cause of genome instability and cancer development. A substantial source of endogenous RS originates from the encounter between the transcription and replication machineries operating on the same DNA template. This occurs predominantly under specific contexts, such as oncogene activation, metabolic stress, or a deficiency in proteins that specifically act to prevent or resolve those transcription-replication conflicts (TRCs). One such protein is Senataxin (SETX), an RNA:DNA helicase involved in resolution of TRCs and R-loops. Here we identify a synthetic lethal interaction between SETX and proteins of the Fanconi anemia (FA) pathway. Depletion of…
Role of the colored noise in a FitzHugh-Nagumo system driven by a periodic signal
2007
During these last years the interest in neuronal dynamics increased. The study of this kind of system has been carried out by using the FitzHugh-Nagumo (FHN) model that is a simplified modification of the Hodgkin-Huxley model. Many interesting phenomena can be observed in the presence of fluctuations: modification of detection threshold by manipulation of noisy parameters (FHN model), noise-induced activation and coeherence resonance for suitable noise amplitude (absence of periodic signal), resonant activation for high periodic signals and noise reduction, intrinsic stochastic resonance (ISR) in Hodgkin-Huxley neuron and the enhancement of a weak signal by tuning the subthreshold intrinsic…
Analyzing the role of soil water retention curve in slope stability for differently structured soils
2016
We analyzed the mechanical and hydrological effects of soil water retention curve (SWRC) models on the slope stability analyses of soils with unimodal and bimodal behavior. We used three different SWRC models, two bimodal models (Ross&Smettem and Dexter) vs. the traditional van Genuchten model, to evaluate the Factor of Safety (FS) of a simple designed hillslope, based on the Bishop approach for unsaturated soils. Two formulations for term were also considered. Results indicate that in cases of clayey soils, changes in FS obtained with different SWRC models especially at soil moisture values within the residual zone. In sandy soils the choice of formulations can be more important…
BIOLOGICAL STABILITY ANALYSIS OF THE OFMSW MATRIX TREATED IN A COMPOSTING PLANT
2011
Composting, mechanical-biological treatment (MBT), biodrying and biostabilization processes of pre-selected organic fraction from municipal solid wastes (OFMSW) have recently become important in municipal solid waste management. Through these treatment processes the potential impacts of the produced wastes and the volume to treat in the landfill are strongly reduced. However, the residual organic matter contained in the treated fraction may provide negative effects when e.g. the treated OFMSW is used as compost. The biological stability degree of the treated matrix provides usefull informations for the final use of it. Indeed, knowing the degree of biological stability of the compost makes …
ANALISI DEL PROCESSO DI ROTTURA PER SOLLEVAMENTO DEL FONDO DI SCAVI CONTROVENTATI
2012
Nella presente nota si riportano i principali risultati di un’analisi sui meccanismi di rottura per sollevamento del fondo di scavi sostenuti da diaframmi. È stata studiata l’influenza su tali meccanismi di fattori quali: lo stato tensionale iniziale; il rapporto tra larghezza e altezza dello scavo; la profondità d’infissione dei diaframmi al di sotto del fondo dello scavo; la dissipazione delle sovrappressioni interstiziali. I risultati ottenuti indicano che i metodi correntemente utilizzati per la valutazione delle condizioni di stabilità del fondo scavo, basati sull’analisi in termini di tensioni totali e sull’impiego della resistenza non drenata su possono portare a sopravvalutare le co…