Search results for "Stability"
showing 10 items of 3085 documents
Active queue management stability in multiple bottleneck networks
2004
In this paper, we show that the active queue management (AQM) controllers, usually configured on a single bottleneck basis, may not prevent instability in the presence of multiple bottlenecks. We justify this result through a multiple bottleneck model.
Bistable spin-crossover nanoparticles showing magnetic thermal hysteresis near room temperature
2007
We have demonstrated that the reverse micelle technique can be applied to polymeric spin-crossover systems, such as [Fe(Htrz)2(trz)](BF4), to control the growth of the crystallites. Small nanoparticles of diameters around 10 nm and narrow size distribution were obtained. It is easy to envision that, by modifying the synthetic procedure, the size and critical temperatures of these nanoparticles can be tuned. On one hand, different ratios of solvent, water, and surfactants will lead to different micelle sizes, which will affect the particle size and, maybe, the magnetic properties. On the other hand, the critical temperatures can be lowered towards room temperature by changing the composition…
A new type of railway monoblock sleeper
2012
A railway sleeper is the structural element which transversally connects the two rails by means of fastening components, thus ensuring its gauge. Its additional function is to firmly secure the track to the ballast and to distribute the loads transferred from railway carriages to the ballast. One of the most important parameters in the characterisation of performances given by railway sleepers is represented by their lateral resistance to the sliding: the higher its value increases, the better the track stability becomes, thus reducing the frequency of maintenance interventions necessary to keep the superstructure efficient. This article focuses on the performance profile of an innovative r…
Magnetic octupole moment of Yb-173 using collinear laser spectroscopy
2021
The hyperfine constants of the $4{f}^{14}6s6p^{3}P_{2}^{o}$ state in neutral Yb have been measured using three different dipole transitions. This state was recently shown to have a comparatively large hyperfine magnetic octupole splitting, and thus a puzzlingly large magnetic octupole moment. The measurement is performed using collinear laser spectroscopy on a fast atomic beam, which provides a straightforward route to probing long-lived metastable atomic states with high resolution. From the combined analysis of all three lines we find no significant evidence for a nonzero octupole moment in $^{173}\mathrm{Yb}$.
Determinants of intracellular RNA pharmacokinetics: Implications for RNA-based immunotherapeutics
2011
RNAs with optimized properties are increasingly investigated as a tool to deliver the genetic information of complete antigens into professional antigen-presenting dendritic cells for HLA haplotype-independent antigen-specific vaccination against cancer. As the dose of the antigen and duration of its presentation are critical factors for generating strong and sustained antigen-specific immune responses, improvement of the immunobioavailability of RNA-based vaccines has been a recurrent subject of research. Substantial increase of the amount of antigen produced from RNA can be achieved by optimizing RNA stability and translational efficiency. Both features are determined by cis-acting elemen…
Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids
2005
Dietary vegetable oils and fish oils rich in PUFA (polyunsaturated fatty acids) exert hypocholesterolaemic and hypotriglyceridaemic effects in rodents. The plasma cholesterol-lowering properties of PUFA are due partly to a diminution of cholesterol synthesis and of the activity of the rate-limiting enzyme HMG-CoA reductase (3-hydroxy-3-methylglutaryl-CoA reductase). To better understand the mechanisms involved, we examined how tuna fish oil and individual n−3 and n−6 PUFA affect the expression of hepatic FPP synthase (farnesyl diphosphate synthase), a SREBP (sterol regulatory element-binding protein) target enzyme that is subject to negative-feedback regulation by sterols, in co-ordination …
mRNA as a versatile tool for exogenous protein expression.
2012
Several viral and non-viral vectors have been developed for exogenous protein expression in specific cells. Conventionally, this purpose is achieved through the use of recombinant DNA. But mainly due to the risks associated with permanent genetic alteration of cells, safety and ethical concerns have been raised for the use of DNA-based vectors in human clinical therapy. In the last years, synthetic messenger RNA has emerged as powerful tool to deliver genetic information. RNA vectors exhibit several advantages compared to DNA and are particularly interesting for applications that require transient gene expression. RNA stability and translation efficiency can be increased by cis-acting struc…
Synthetic mRNAs with Superior Translation and Stability Properties
2012
The translational efficiency and stability of synthetic mRNA in both cultured cells and whole animals can be improved by incorporation of modified cap structures at the 5'-end. mRNAs are synthesized in vitro by a phage RNA polymerase transcribing a plasmid containing the mRNA sequence in the presence of all four NTPs plus a cap dinucleotide. Modifications in the cap dinucleotide at the 2'- or 3'-positions of m(7)Guo, or modifications in the polyphosphate chain, can improve both translational efficiency and stability of the mRNA, thereby increasing the amount and duration of protein expression. In the context of RNA-based immunotherapy, the latter is especially important for antigen producti…
Pre-Analytical Determination of the Effect of Extended Warm or Cold Ischaemia on RNA Stability in the Human Ileum Mucosa
2015
The use of banked human tissue, obtained with informed consent after elective surgical procedures, represents a powerful model for understanding underlying mechanisms of diseases or therapeutic interventions and for establishing prognostic markers. However, donated tissues typically have varying times of warm ischaemia in situ due to blood arrest or cold ischaemia due to procurement and transportation. Hence, before using these tissues, it is important to carry out pre-analytical studies to ensure that they are representative of the in vivo state. In particular, tissues of the gastrointestinal tract have been thought to have low RNA stability. Therefore, this study aimed to determine if ext…
Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune r…
2010
Vaccination with in vitro transcribed RNA coding for tumor antigens is considered a promising approach for cancer immunotherapy and has already entered human clinical testing. One of the basic objectives for development of RNA as a drug is the optimization of immunobioavailability of the encoded antigen in vivo. By analyzing the effect of different synthetic 5' mRNA cap analogs on the kinetics of the encoded protein, we found that m(2)(7,2'-O)Gpp(S)pG (beta-S-ARCA) phosphorothioate caps, in particular the D1 diastereoisomer, profoundly enhance RNA stability and translational efficiency in immature but not mature dendritic cells. Moreover, in vivo delivery of the antigen as beta-S-ARCA(D1)-c…