Search results for "Stem Cell"

Allogeneic stem cell transplantation for renal cell carcinoma

2011

Allogeneic hematopoietic stem cell transplantation from a compatible donor has been utilized as adoptive immunotherapy in metastatic, cytokine-refractory renal cell carcinoma (RCC). Since the year 2000, several investigators have established that RCC is susceptible to a graft-versus-tumor effect: they reported that patients with renal cancer may have partial or complete disease responses, in the 20-40% range, after allogeneic transplantation following a reduced-intensity regimen. However, transplant-related mortality is still high in the 10-20% range, and responses are rarely durable. Experimental evidence suggests that donor-derived T cells and natural killer cells are the main mediators o…

Detection of Cancer Stem Cells Using AC133 Antibody

2012

The cancer stem cell (CSC) model postulates that tumors are formed and maintained by a small population of undifferentiated cells, characterized by unique self-renewal properties and malignant potential. As CSCs survive chemotherapy inducing tumor recurrence, the specific targeting and eradication of these cells represents an important therapeutic challenge in cancer cure. Recent findings on CD133, a pentaspan transmembrane glycoprotein, discuss its application in the identification and isolation of SCs from cancerous tissues. However, the appropriateness of this protein constitutes an ongoing discussion. The expression pattern is debated and several issues highlight the need for additional…

Perinatal stem cells patents and applications: Regenerative medicine, tissue repair, immune modulation

2013

Recent patents and advances in hepatocyte-like cells differentiation by mesenchymal stem cells

2013

Chronic liver diseases constitute one of the main causes of death in western countries. Orthotopic liver transplantation still remains the final therapeutic approach to these diseases, but alternative therapeutic strategies are actively researched. Hepatocyte transplantation is considered a promising approach, even if this technique presents many limitations. These factors boosted the research for alternative cell sources to derive functional hepatocytes. In the last years, research on basic biology and differentiative ability of adult, embryonic and perinatal stem cells has constantly increased. The term "perinatal" indicates stem cell populations derived from foetal sources such as placen…

Towards an ideal source of mesenchymal stem cell isolation for possible therapeutic application in regenerative medicine.

2014

Background. The possibility of obtaining mesenchymal stem cells (MSCs) from fetal tissue such as amniotic fluid, chorionic villi and placenta is well-known and a comparison between MSCs originating in different sources such as fetal tissue and those from bone marrow in terms of yield and function is a topical issue. The mesenchymal stem cells isolated from bone marrow are well-characterized. Unfortunately the low quantitative yield during isolation is a major problem. For this reason, other tissue sources for MSCs are of paramount importance. Conclusion. In this review, starting from a description of the molecular and cellular biology of MSCs, we describe alternative sources of isolation ot…

Molecular characterization of cancer stem cells in anaplastic thyroid carcinoma

2009

Successful subretinal delivery and monitoring of MicroBeads in mice

2013

BACKGROUND: To monitor viability of implanted genetically engineered and microencapsulated human stem cells (MicroBeads) in the mouse eye, and to study the impact of the beads and/or xenogenic cells on retinal integrity. METHODOLOGY/PRINCIPAL FINDINGS: MicroBeads were implanted into the subretinal space of SV126 wild type mice using an ab externo approach. Viability of microencapsulated cells was monitored by noninvasive retinal imaging (Spectralis™ HRA+OCT). Retinal integrity was also assessed with retinal imaging and upon the end of the study by light and electron microscopy. The implanted GFP-marked cells encapsulated in subretinal MicroBeads remained viable over a period of up to 4 mont…

Higher oxidative stress in human dental pulp stem cells cultured at 21% O2 compared to 5% O2

2012

Stimulation of Wound Revascularization by Adenosine Receptor Activation

2010

Adenosine is an endogenous mediator implicated in wound healing. The exact mechanisms and receptors involved are still under evaluation. We have observed that topical application of a selective adenosine A2A receptor agonist promotes wound healing in experimental animals, both healthy and with impaired healing. Histological analysis revealed that adenosine promoted granulation tissue formation, with increased cellularity, matrix deposition, and vessel density. Adenosine may exert effects on many different cell types that finally result in promotion of angiogenesis and, furthermore, homing of endothelial precursor cells into sites of injury. We will review the effect of adenosine receptor ac…

Priming with proangiogenic growth factors and endothelial progenitor cells improves revascularization in linear diabetic wounds

2014

In the present study, we investigated whether proangiogenic growth factors and endothelial progenitor cells (EPCs) induce favourable effects on cutaneous incisional wound healing in diabetic mice. The proangiogenic effects of human EPCs were initially analyzed using a HUVEC in vitro angiogenesis assay and an in vivo Matrigel assay in nude mice (n=12). For the diabetic wound model, 48 Balb/c mice with streptozotocin (STZ)-induced diabetes were divided randomly into 4 groups (12 mice in each group). Subsequently, 3, 5 and 7 days before a 15-mm full-thickness incisional skin wound was set, group 1 was pre-treated subcutaneously with a mixture of vascular endothelial growth factor (VEGF)/basic …