Search results for "Supplement"

showing 10 items of 499 documents

Human Intrinsic Factor Expression for Bioavailable Vitamin B12 Enrichment in Microalgae

2018

Dietary supplements and functional foods are becoming increasingly popular complements to regular diets. A recurring ingredient is the essential cofactor vitamin B12(B12). Microalgae are making their way into the dietary supplement and functional food market but do not produce B12, and their B12 content is very variable. In this study, the suitability of using the human B12-binding protein intrinsic factor (IF) to enrich bioavailable B12 using microalgae was tested. The IF protein was successfully expressed from the nuclear genome of the model microalga Chlamydomonas reinhardtii and the addition of an N-terminal ARS2 signal peptide resulted in efficient IF secretion to the medium. Co-abunda…

0301 basic medicineSignal peptide<i>Chlamydomonas</i>; vitamin B<sub>12</sub>; cobalamin; intrinsic factor; microalgae; nuclear transformation; recombinant protein; dietary supplements; functional foodsChlamydomonaChlamydomonas reinhardtiiArticleGeneral Biochemistry Genetics and Molecular Biologydietary supplements03 medical and health sciencesIngredientnuclear transformationFunctional foodpolycyclic compoundsVitamin B12Food sciencecobalaminlcsh:QH301-705.5functional foodsIntrinsic factorGeneral Immunology and MicrobiologybiologymicroalgaeChlamydomonasChlamydomonasnutritional and metabolic diseasesvitamin B12biology.organism_classificationBioavailability030104 developmental biologylcsh:Biology (General)dietary supplementintrinsic factorGeneral Agricultural and Biological Sciencesrecombinant proteinBiology; Volume 7; Issue 1; Pages: 19
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REGGAE : a novel approach for the identification of key transcriptional regulators

2019

Abstract Motivation Transcriptional regulators play a major role in most biological processes. Alterations in their activities are associated with a variety of diseases and in particular with tumor development and progression. Hence, it is important to assess the effects of deregulated regulators on pathological processes. Results Here, we present REGulator-Gene Association Enrichment (REGGAE), a novel method for the identification of key transcriptional regulators that have a significant effect on the expression of a given set of genes, e.g. genes that are differentially expressed between two sample groups. REGGAE uses a Kolmogorov–Smirnov-like test statistic that implicitly combines assoc…

0301 basic medicineStatistics and ProbabilityTranscription Genetic610Computational biologyBiologyBiochemistry03 medical and health sciencesNeoplasmsHumansTwo sampleMolecular BiologyGeneProbabilitySupplementary dataRegulation of gene expressionSystems Biology500Original PapersComputer Science Applications004Computational Mathematics030104 developmental biologyComputational Theory and MathematicsGene Expression RegulationKey (cryptography)Identification (biology)FemaleSoftware
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Taste perception and its effects on oral nutritional supplements in younger life phases.

2018

Purpose of review The current review summarizes the importance of taste perception with regard to acceptance of oral nutritional supplements (ONS) in young children. We also shed light on how basic tastes may influence the orosensory detection of ONS in the light of genetic variations, encoding for different taste modalities, particularly for sweet and bitter (and fat), in children. Recent findings Single nucleotide polymorphism (SNP) of bitter and sweet taste receptor genes, that is, respectively, TAS2R38 and T1R2/T1R3, may influence orosensory perception of ‘bitter-made-sweet’ ONS. The SNP of fat taste receptor gene, that is, CD36, might communicate with bitter taste perception. The emerg…

0301 basic medicineTastePediatric ObesityAdolescentGenotypemedia_common.quotation_subjectPopulationMedicine (miscellaneous)PhysiologyPolymorphism Single NucleotideReceptors G-Protein-Coupled03 medical and health sciencesFood Preferencesstomatognathic systemTaste receptorPerceptionmedicineHumanseducationChildmedia_commoneducation.field_of_study030109 nutrition & dieteticsNutrition and Dieteticsbusiness.industryInfant Newbornfood and beveragesInfantTaste Perceptionmedicine.diseaseObesityDietary FatsSodium salt030104 developmental biologyTAS2R38Child PreschoolTasteDietary SupplementsTaste aversionNutrition Therapybusinesspsychological phenomena and processesCurrent opinion in clinical nutrition and metabolic care
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Taste Perception of Nutrients Found in Nutritional Supplements: A Review

2019

open access review pii: nu11092050.; International audience; Nutritional supplements are prescribed when one's nutritional status is not conducive to good health. These foodstuffs constitute concentrated sources of nutrients such as vitamins, minerals, amino acids, and fatty acids. For nutritional supplements to be effective, patients must consume the amount that has been prescribed for the recommended period of time. Therefore, special attention must be given to the sensory attributes of these products. Indeed, the presence of active compounds can cause an off-taste or aftertaste. These negative sensations can lead to a reduction in the consumption of nutritional supplements and reduce the…

0301 basic medicineTastenutritional supplements;active compounds;taste;taste receptors;bittermedia_common.quotation_subjectnutritional supplementsgoûtlcsh:TX341-641Reviewcomposé actiftaste03 medical and health sciences0404 agricultural biotechnologyNutrientPerceptionFood and NutritionHumansactive compoundsMedicineFood scienceamertumerécepteur du goûtAftertastemedia_common2. Zero hungerNutrition and Dieteticsbusiness.industryTaste PerceptionNutritional statusNutrients04 agricultural and veterinary sciencesbitter040401 food science3. Good health030104 developmental biologycomplement nutritionnelAlimentation et NutritionDietary Supplementstaste receptorsbusinesslcsh:Nutrition. Foods and food supply[SDV.AEN]Life Sciences [q-bio]/Food and NutritionFood ScienceNutrients
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Quality and antioxidant response of gilthead seabream (Sparus aurata L.) to dietary supplements of fenugreek (Trigonella foenum graecum) alone or com…

2017

The present study was conducted to determine the potential effect of the dietary intake of fenugreek (Trigonella foenum graecum) seeds alone or in combination with Bacillus licheniformis, Lactobacillus plantarum or B. subtilis on gilthead seabream quality and antioxidant response after 2 and 3 weeks of experimental feeding. The results showed that the supplements did not affect the percentage of the fatty acid profiles of muscle, demonstrating that all the additives tested can be administrated without any negative effect on biochemical composition and quality of gilthead seabream. The quantification of thiobarbituric acid reactive substances in muscle demonstrated the significant beneficial…

0301 basic medicineTrigonellaFenugreek seedThiobarbituric acidAquatic ScienceProbioticTeleostsGilthead seabream (Sparus aurata L.)law.inventionSuperoxide dismutase03 medical and health sciencesIngredientchemistry.chemical_compoundProbioticRandom AllocationAdjuvants ImmunologicSettore AGR/20 - ZoocolturelawEnvironmental ChemistryAnimalsBacillus licheniformisFood scienceSettore BIO/06 - Anatomia Comparata E Citologiachemistry.chemical_classificationbiologyPlant ExtractsAntioxidant statuProbioticsFatty acid04 agricultural and veterinary sciencesGeneral Medicinebiology.organism_classificationAnimal FeedImmunity InnateSea BreamDietAntioxidant-related gene expression030104 developmental biologyTrigonellachemistryBiochemistryCatalaseDietary Supplements040102 fisheriesbiology.protein0401 agriculture forestry and fisheriesLactobacillus plantarumBacillus subtilisLactobacillus plantarumFishshellfish immunology
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Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subse…

2018

Despite transferrin being the main circulating carrier of iron in body fluids, and iron overload conditions being known to worsen stroke outcome through reactive oxygen species (ROS)-induced damage, the contribution of blood transferrin saturation (TSAT) to stroke brain damage is unknown. The objective of this study was to obtain evidence on whether TSAT determines the impact of experimental ischemic stroke on brain damage and whether iron-free transferrin (apotransferrin, ATf)-induced reduction of TSAT is neuroprotective. We found that experimental ischemic stroke promoted an early extravasation of circulating iron-loaded transferrin (holotransferrin, HTf) to the ischemic brain parenchyma.…

0301 basic medicineU-PAGE urea-polyacrylamide gel electrophoresisMaleClinical BiochemistryExperimental strokeBiochemistryBrain IschemiaBrain ischemia0302 clinical medicineADC apparent diffusion coefficientApotransferrinDWI diffusion-weighted imagingTANDEM-1 Thrombolysis and Deferoxamine in Middle Cerebral Artery Occlusion clinical trialrHTf rat HTfrATf rat ATflcsh:QH301-705.5chemistry.chemical_classificationNeuronslcsh:R5-920ChemistryTransferrinExtravasationNS21 a medium supplement to grow neuronspDAPK-1 phosphorylated anti-death-associated protein kinase 1NeuroprotectionStrokeWB Western blotFemalemedicine.symptomlcsh:Medicine (General)Research PaperhHTf human HTfPC12 cell line derived from a pheochromocytoma of the rat adrenal medullamedicine.medical_specialtyIron OverloadBBB blood-brain barrierNMDAR N-methyl-D-aspartate receptorDCF dihydrofluoresceinIronWGA wheat germ agglutininHTf holotransferrinTransferrin receptorBrain damageTfR transferrin receptorDeferoxamineNeuroprotectionPI propidium iodide03 medical and health sciencesBrain damageCM conditioned mediumROS reactive oxygen speciesInternal medicine4-HNE 4-hydroxynonenalTf transferrinReceptors TransferrinmedicineFeRhoNoxTM-1 probe to detect Fe2+AnimalsHumansATf apotransferrinCM-H2DCFDA 5-chloromethyl-27-dichlorodihydrofluorescein diacetateMCAO middle cerebral artery occlusionDMT-1 divalent metal transporterB-27 a medium supplement to grow neuronsReactive oxygen speciesNMDA N-methyl-D-aspartateTSAT blood transferrin saturationTransferrin saturationBlood transferrin saturation (TSAT)Organic ChemistryNIR near infraredReactive oxygen species (ROS)medicine.diseasepMCAO permanent middle cerebral artery occlusionRatsPWI perfusion-weighted imaging030104 developmental biologyEndocrinologylcsh:Biology (General)TransferrinDAPK-1 anti-death-associated protein kinaseOGD oxygen/glucose deprivationTTC 235-triphenyl-tetrazolium chlorideLipid PeroxidationMCA middle cerebral arteryApoproteinsReactive Oxygen SpeciesMRI magnetic resonance imagingtMCAO transient middle cerebral artery occlusion030217 neurology & neurosurgeryhATf human ATf
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Relationship between adipic acid concentration and the core symptoms of autism spectrum disorders.

2016

Dicarboxylic acids are an important source of information about metabolism and potential physiopathological alterations in children with autism spectrum disorders (ASDs). We measured the concentration between dicarboxylic adipic and suberic acids in children with an ASD and typically-developing (TD) children and analyzed any relationships between the severity of the core symptoms of ASDs and other clinical features (drugs, supplements, drugs, or diet). The core symptoms of autism were evaluated using the DSM-IV criteria, and adipic acid and suberic acid were measured in urine samples. Overall, no increase in the concentration of adipic acid in children with ASDs compared to TD children, how…

0301 basic medicineVitamin bMalePediatricsmedicine.medical_specialtyAdolescentAutism Spectrum DisorderAdipatesUrinebehavioral disciplines and activitiesSeverity of Illness Index03 medical and health scienceschemistry.chemical_compound0302 clinical medicineInternal medicinemental disordersmedicineHumansDicarboxylic AcidsChildBiological PsychiatryAdipic acidbusiness.industrySignificant differenceMetabolismmedicine.diseaseDiagnostic and Statistical Manual of Mental DisordersPsychiatry and Mental health030104 developmental biologyEndocrinologychemistryCase-Control StudiesChild PreschoolDietary SupplementsVitamin B ComplexAutismFemaleCore symptomsCaprylatesbusinessSuberic acid030217 neurology & neurosurgeryPsychiatry research
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Vitamin D and Cardiovascular Health

2021

The principal source of vitamin D in humans is its biosynthesis in the skin through a chemical reaction dependent on sun exposure. In lesser amounts, the vitamin can be obtained from the diet, mostly from fatty fish, fish liver oil and mushrooms. Individuals with vitamin D deficiency, defined as a serum level of 25 hydroxyvitamin D < 20 ng/dl, should be supplemented. Vitamin D deficiency is a prevalent global problem caused mainly by low exposure to sunlight. The main role of 1,25 dihydroxyvitamin D is the maintenance of calcium and phosphorus homeostasis. However, vitamin D receptors are found in most human cells and tissues, indicating many extra-skeletal effects of the vitamin, particula…

0301 basic medicineVitaminmedicine.medical_specialtyVitamina DEnfermedad cardiovascularchemistry.chemical_element030209 endocrinology & metabolismCalciumCritical Care and Intensive Care Medicinemedicine.disease_causevitamin D deficiencyNitric oxide03 medical and health scienceschemistry.chemical_compound0302 clinical medicineImmune systemInternal medicineHipertensiónmedicineVitamin D and neurologyHumansSupplementsVitamin D030109 nutrition & dieteticsNutrition and DieteticsEnfermedad coronariabusiness.industryEndothelial functionVitamin D Deficiencymedicine.diseaseCardiovascular diseaseCoronary heart diseaseEndocrinologyDietéticachemistryCardiovascular DiseasesNutriciónHypertensionNarrative ReviewbusinessHomeostasisOxidative stress
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Supplementary medication in multiple sclerosis: Real-world experience and potential interference with neurofilament light chain measurement

2020

Background As vitamins and dietary supplements are obtainable without prescription, treating physicians often ignore their intake by patients with multiple sclerosis (MS) and may therefore miss potential adverse effects and interactions. Objective We aimed to assess the spectrum and intake frequency of supplementary medication in a cohort of MS patients and to analyse the effect of biotin intake on measurement of serum neurofilament light chain (sNfL), an emerging marker of disease activity. Methods MS patients visiting our neurology outpatient clinic completed a questionnaire on their past or present use of vitamins or dietary supplements. In addition, the impact of two different doses of …

0301 basic medicinebusiness.industryMultiple sclerosisNeurofilament lightvitamin DvitaminsInterference (genetic)medicine.diseaseBioinformaticsOriginal Research PaperMultiple sclerosisdietary supplements03 medical and health sciencesCellular and Molecular Neuroscience030104 developmental biology0302 clinical medicinebiotinmedicineVitamin D and neurologyNeurology (clinical)Medical prescriptionbusiness030217 neurology & neurosurgeryMultiple Sclerosis Journal - Experimental, Translational and Clinical
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Melatonin reduces inflammatory response in human intestinal epithelial cells stimulated by interleukin‐1β

2019

Melatonin is the main secretory product of the pineal gland, and it is involved in the regulation of periodic events. A melatonin production independent of the photoperiod is typical of the gut. However, the local physiological role of melatonin at the intestinal tract is poorly characterized. In this study, we evaluated the anti-inflammatory activities of melatonin in an in vitro model of inflamed intestinal epithelium. To this purpose, we assessed different parameters usually associated with intestinal inflammation using IL-1 beta-stimulated Caco-2 cells. Differentiated monolayers of Caco-2 cells were preincubated with melatonin (1 nmol/L-50 mu mol/L) and then exposed to IL-1 beta. After …

0301 basic medicineendocrine systemmedicine.medical_specialtyantioxidantDNA damageInterleukin-1betainflammatory bowel diseasesdietary supplementsMelatonin03 medical and health sciencesPineal gland0302 clinical medicineEndocrinologyCell surface receptorSettore BIO/10 - BiochimicaInternal medicinemedicineHumansMelatoninInflammationN-acetyl-5-methoxy-tryptamineInterleukin-6Chemistryantioxidants; dietary supplements; DNA damage; DNA methylation; inflammatory bowel diseases; N-acetyl-5-methoxy-tryptamine; NF-κB activationInterleukin-8AntagonistCell DifferentiationEpithelial CellsDNA MethylationSettore CHIM/08 - Chimica FarmaceuticaIntestinal epitheliumIntestinesSettore BIO/18 - Geneticaantioxidants030104 developmental biologyEndocrinologymedicine.anatomical_structureNF-κB activationCyclooxygenase 2dietary supplementParacellular transportDNA damageCaco-2 CellsLuzindolehormones hormone substitutes and hormone antagonists030217 neurology & neurosurgerySignal Transductionmedicine.drugJournal of Pineal Research
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