Search results for "Surfactant"
showing 10 items of 397 documents
Some observations on the fluorometric determination of metallic elements in micellar media
1989
Abstract Experimental evidence is given allowing us to interpret why the detection limits obtained in micellar media are higher than those expected according to fluorescence enhancements for the niobium-lumogallion-tartrate system in the presence of different nonionic surfactants. The residual fluorescence of the surfactant solution and the competitive interaction with micelles between complexes and free ligands were studied. Near the critical micelle concentration of surfactants, remarkable changes in the emission spectra were observed in the case of both complex and free ligand solutions, which indicate the existence of interactions between the chemical system and surfactant monomers and …
Performance of short-chain alcohols versus acetonitrile in the surfactant-mediated reversed-phase liquid chromatographic separation of β-blockers
2010
Organic solvents are traditionally added to micellar mobile phases to achieve adequate retention times and peak profiles, in a chromatographic mode which has been called micellar liquid chromatography (MLC). The organic solvent content is limited to preserve the formation of micelles. However, at increasing organic solvent contents, the transition to a situation where micelles do not exist is gradual. Also, there is no reason to neglect the potentiality of mobile phases containing only surfactant monomers instead of micelles (high submicellar chromatography, HSC). This is demonstrated here for the analysis of β-blockers. The performance of four organic solvents (methanol, ethanol, 1-propano…
Comparison of surfactant-mediated liquid chromatographic modes with sodium dodecyl sulphate for the analysis of basic drugs
2020
In reversed-phase liquid chromatography (RPLC), basic drugs are positively charged at the usual working pH range and interact with free anionic silanols present in conventional silica-based stationary phases. This translates into stronger retention and tailed and broadened peaks. This problem can be resolved by the addition of reagents to the mobile phase that are adsorbed on the stationary phase, avoiding the access of solutes to silanols. Among these additives, surfactants under micellar conditions have provided good silanol suppressing potency through the technique known as micellar liquid chromatography (MLC). The most common example of this is anionic sodium dodecyl sulphate (SDS). Whe…
Implementation of gradients of organic solvent in micellar liquid chromatography using DryLab®: Separation of basic compounds in urine samples
2014
In micellar liquid chromatography (MLC), chromatographic peaks are more evenly distributed compared to conventional reversed-phase liquid chromatography (RPLC). This is the reason that most procedures are implemented using isocratic elution. However, gradient elution may be still useful in MLC to analyse mixtures of compounds within a wide range of polarities, decreasing the analysis time. Also, it benefits the determination of moderately to low polar compounds in physiological fluids performing direct injection: an initial micellar eluent with a low organic solvent content, or a pure micellar (without surfactant) solution, will provide better protection of the column against the proteins i…
Retention mechanisms for basic drugs in the submicellar and micellar reversed-phase liquid chromatographic modes.
2008
The reversed-phase liquid chromatographic (RPLC) behavior (retention, elution strength, selectivity, efficiency, and peak asymmetry) for a group of basic drugs (beta-blockers), with mobile phases containing the anionic surfactant sodium dodecyl sulfate (SDS) and acetonitrile, revealed different separation environments, depending on the concentrations of both modifiers: hydro-organic, submicellar at low surfactant concentration and high concentration of organic solvent, micellar, and submicellar at high concentration of both surfactant and organic solvent. In the surfactant-mediated modes, the anionic surfactant layer adsorbed on the stationary phase interacts strongly with the positively ch…
HYDRO-ORGANIC AND MICELLAR-ORGANIC REVERSED-PHASE LIQUID CHROMATOGRAPHIC PROCEDURES FOR THE EVALUATION OF SULPHONAMIDES IN PHARMACEUTICALS
2001
Two reversed-phase liquid chromatographic procedures were developed for the analysis of ten pharmaceutical formulations used in medicine and veterinary, which contained one of the following sulphonamides: sulphacetamide, sulphadiazine, sulphaguanidine, sulphamethazine, sulphamethizole, sulphamethoxazole, or sulphathiazole. In both chromatographic modes, the same C18 column was utilized and the mobile phase contained a low amount of acetonitrile and citric buffer at pH 3.0. In one of the procedures, the surfactant sodium dodecyl sul phate was added to the mobile phase. The presence of micelles allowed a decrease in the amount of organic solvent from 11% to 6%, and facilitated sample dissolut…
Volumes and compressibilities of pentanol in aqueous dodecyltrimethylammonium bromide solutions at 15, 25 and 35°C
1990
Ultrasonic velocities and densities of the water-dodecyltrimethylammonium bromide (DTAB)-pentanol (PentOH) ternary system were measured at 15, 25 and 35°C as a function of the surfactant and alcohol concentrations. The apparent molar volumes and isentropic compressibilities of PentOH were calculated. The standard partial molar volumes increase with surfactant concentration continuously whereas the standard partial molar isentropic compressibilities show sharp changes in slope at about 0.25 mol-kg−1 DTAB, which can be ascribed to a micellar structural transition. The volume data for alcohol in micellar solutions were treated by a model reported for the distribution of polar additives between…
Influence of the addition of modifiers on solute-micelle interaction in hybrid micellar liquid chromatography
1998
In reversed-phase micellar liquid chromatography (MLC) organic modifiers are usually added to the mobile phase to modify the eluent strength and to increase the efficiency of the chromatographic peaks. The effect of the modifiers methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, acetonitrile and tetrahydrofuran on the critical micelle concentration (cmc) of the anionic surfactant sodium dodecylsulphate (SDS) has been studied to enable understanding of the interactions between solute, micelles and bulk liquid in such hybrid eluents. Methanol, acetonitrile and tetrahydrofuran increased thecmc, whereas the other alcohols reduced its value. For butanol and pentanol, which partition into the…
Chromatographic Efficiency in Micellar Liquid Chromatography: Should it Be Still a Topic of Concern?
2013
Micellar liquid chromatography (MLC) was first proposed as an attractive alternative to avoid the use of organic solvents. It was soon apparent that pure micellar solutions yield poor efficiencies. This problem was remediated by the addition of a small amount of an organic solvent. However, the general opinion of the poor peak shape has prevailed as a handicap for MLC, in spite of the fact that the hybrid mode often offers similar or even improved efficiencies (for basic compounds) relative to that attained in the hydro-organic mode. Only the efficiencies for apolar non-ionizable compounds are still clearly inferior. This work describes the type of interactions and polarity changes with org…
Comparison of the performance of non-ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs
2011
Surfactants added to the mobile phases in reversed-phase liquid chromatography (RPLC) give rise to a modified stationary phase, due to the adsorption of surfactant monomers. Depending on the surfactant nature (ionic or non-ionic), the coated stationary phase can exhibit a positive net charge, or just change its polarity remaining neutral. Also, micelles in the mobile phase introduce new sites for solute interaction. This affects the chromatographic behavior, especially in the case of basic compounds. Two surfactants of different nature, the non-ionic Brij-35 and the anionic sodium dodecyl sulfate (SDS) added to water or aqueous-organic mixtures, are here compared in the separation of basic …